Combining asymmetrical flow field-flow fractionation with on- and off-line fluorescence detection to examine biodegradation of riverine dissolved and particulate organic matter

Lee, Sang Tak; Yang, Beomjoo; Kim, Jin Yong; Park, Ji‐Hyung; Moon, Myeong Hee

doi:10.1016/j.chroma.2015.07.074

Cited by 9 publications

(3 citation statements)

References 47 publications

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“…So far, a variety of methods such as adsorption, [3][4][5][6][7] biodegradation, 8 oxidation, 9 and membrane ltration 10 have been employed to remove different organic micropollutants from wastewater. Amongst these techniques, adsorption using solid adsorbents is an effective method for the removal of organic micropollutants from water.…”

Section: Introductionmentioning

confidence: 99%

Preparation of cationic polymeric nanoparticles as an effective adsorbent for removing diclofenac sodium from water

et al. 2017

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Section: Introductionmentioning

confidence: 99%

Preparation of cationic polymeric nanoparticles as an effective adsorbent for removing diclofenac sodium from water

et al. 2017

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“…3c and d, the half bandwidth for membrane with 0.3 kDa of MWCO was smaller than that of 1 kDa of MWCO both for UV and fluorescent fractograms, indicating that 0.3 kDa of MWCO reserved more small molecules. Since aquatic DOMs contained usually a broad MW distribution and abundant small molecules (Lee et al 2015; Kozyatnyk et al 2016), membranes with 0.3 kDa of MWCO could obtain more information on the MW distribution.…”

Section: Assessment and Discussionmentioning

confidence: 99%

Characterization and modification of the molecular weight distribution within dissolved organic matter using flow field‐flow fractionation

Pan

2020

Limnology & Ocean Methods

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Dissolved organic matter (DOM) exists ubiquitously in the aquatic environment with varying chemical compositions and a wide molecular weight (MW) distribution, which, as a result, exhibits differing impacts on the behavior and fate of contaminants. The current methods for the MW characterization of DOM usually possess some weaknesses and therefore, some novel techniques are needed. In this study, the feasibility and sensitivity of flow field-flow fractionation (FlFFF) system were studied for the MW characterization of DOMs from various origins. The systematic evaluation of FlFFF indicated that a mobile phase consisting of 10 mM NaCl and 5 mM H 3 BO 3 and with a pH of 8 was optimal, and the fractionation program with a cross-flow rate of 2 mL/min and a focusing time of 2 min resulted in the most efficient MW fractionation. Compared to natural waters, the standard DOM materials possessed a high proportion of humic-like substances and narrow MW distribution. The quantification analysis showed that chromophoric organic ligands were mainly distributed in the high (i.e., 100 kDa-0.45 μm) and low (i.e., 0.3-1 kDa) MW fractions for natural waters, but in the medium (i.e., 1-100 kDa) and low MW fractions for the standard DOM materials. However, the fluorescent humic-like substances were predominantly distributed within the medium and low MW fractions, irrespective of DOM types. This study showed the feasibility and sensibility of FlFFF in fractionating DOM samples, and can be used to quantify the different MW distribution of chromophoric and fluorescent components in standard and natural DOM.

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“…Fluorescence detection (FLD) can be a more sensitive and selective alternative to UV-Vis detection for fluorescent or fluorescently-labeled compounds [42][43][44]. While AF4 has been coupled with FLD to characterize macromolecules [45] such as humic substances [46][47][48][49], proteins [42,43,50], and biopolymers [51], it has not yet been used to quantify drug loading inside the polymeric NPs. Iavicoli et al evaluated the binding of fluorophore-tagged peptides to liposomes by coupling AF4 with either UV-Vis or FLD, but FLD was only used for qualitative confirmation of binding since UV-Vis detection was reported to provide more reproducible and linear quantification of the peptide [52].…”

Section: Introductionmentioning

confidence: 99%

Asymmetric Flow Field-Flow Fractionation (AF4) with Fluorescence Detection for Direct, Real-Time, Size-Resolved Measurements of Drug Release from Polymeric Nanoparticles

Shakiba¹,

Astete²,

Cueto³

et al. 2020

Preprint

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Polymeric nanoparticles (NPs) are typically designed to enhance the efficiency of drug delivery by controlling the drug release rate. Hence, it is critical to obtain an accurate drug release profile. This study presents the first application of asymmetric flow field-flow fractionation (AF4) with fluorescence detection (FLD) to characterize release profiles of fluorescent drugs from polymeric NPs, specifically poly lactic-co-glycolic acid NPs loaded with enrofloxacin (PLGA-Enro NPs). In contrast to traditional release measurements requiring separation of entrapped and dissolved drugs (typically by dialysis) prior to quantification, AF4-FLD provides in situ purification of the NPs from unincorporated drugs, along with direct measurement of the entrapped drug. Size distributions and shape factors are simultaneously obtained by online dynamic and multi-angle light scattering detectors. The AF4-FLD and dialysis approaches were compared to evaluate drug release from PLGA-Enro NPs containing a high proportion (≈ 88%) of unincorporated (burst release) drug at three different temperatures spanning the glass transition temperature (30 °C for PLGA-Enro NPs). The AF4-FLD analysis was able to identify size-dependent release rates across the entire continuous NP size distribution, with smaller NPs showing faster release. The AF4-FLD method also clearly captured the expected temperature dependence of the drug release (from almost no release at 20 °C to rapid release at 37 °C). In contrast, dialysis was not able to distinguish these differences in the extent or rate of release of the entrapped drug because of interferences from the burst release background, as well as the dialysis lag time. A mechanistic diffusion model that integrates data from both AF4-FLD and dialysis further supported the advantages of AF4-FLD to capture the true release rate of entrapped drug and avoid artifacts observed in dialysis. Overall, this study demonstrates the novel application and unique advantages of AF4-FLD methods to obtain direct, size-resolved release profiles of fluorescent drugs from polymeric NPs.

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Combining asymmetrical flow field-flow fractionation with on- and off-line fluorescence detection to examine biodegradation of riverine dissolved and particulate organic matter

Cited by 9 publications

References 47 publications

Preparation of cationic polymeric nanoparticles as an effective adsorbent for removing diclofenac sodium from water

Preparation of cationic polymeric nanoparticles as an effective adsorbent for removing diclofenac sodium from water

Characterization and modification of the molecular weight distribution within dissolved organic matter using flow field‐flow fractionation

Asymmetric Flow Field-Flow Fractionation (AF4) with Fluorescence Detection for Direct, Real-Time, Size-Resolved Measurements of Drug Release from Polymeric Nanoparticles

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