Combining cell sorting with gas chromatography to determine phytoplankton group-specific intracellular dimethylsulphoniopropionate

Archer, Stephen D.; Tarran, Glen A.; Stephens, John A.; Butcher, Lindsay J.; Kimmance, Susan A.

doi:10.3354/ame01464

Cited by 22 publications

(17 citation statements)

References 38 publications

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“…These studies used indirect measurements of DMSP through derivatization to DMS and gas chromatography (GC) detection. We only included measurements from monoculture studies as in situ estimates of species specific DMSP concentrations (e.g., Archer et al ) could be biased by the uptake of dissolved DMSP which can vary significantly by species (Vila‐Costa et al ; Lavoie et al ).…”

Section: Methodsmentioning

confidence: 99%

The role of differential DMSP production and community composition in predicting variability of global surface DMSP concentrations

McParland

Levine

2018

Limnology & Oceanography

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Dimethylsulfoniopropionate (DMSP) is an important labile component of the marine dissolved organic matter pool that is produced by the majority of eukaryotic marine phytoplankton and by many prokaryotes. Despite decades of research, the contribution of different environmental drivers of DMSP production to regional and seasonal variability remains unknown. A synthesis of the current state‐of‐knowledge suggested that approximately half of confirmed DMSP producers are low producers (intracellular DMSP < 50 mM). Low DMSP producers (LoDPs; e.g., diatoms) were shown to strongly regulate intracellular DMSP concentrations (~ 16‐fold change) as a predictable function of nutrient stress. By comparison, high DMSP producers (HiDPs; e.g., coccolithophores) showed very little response (~ 1.5‐fold change). To assess the importance of differential DMSP production by low and high producers, DMSP concentrations were predicted for two time‐series sites (a high‐ and low‐productivity site) and for the global ocean by explicitly incorporating both community composition and mechanistic nutrient stress. Despite large, predictable intracellular DMSP changes, low producers contributed less than 5% to global DMSP. This indicates that, while variations in DMSP production by low producers could be important for predicting microbial interactions and low producer physiology, it is not necessary for predicting global DMSP concentrations. Our analysis suggests that community composition, particularly HiDP biomass, is the dominant driver of variability in in situ DMSP concentrations, even in low‐productivity regions where high producers are typically the subdominant group. Accurate predictions of in situ DMSP concentrations require improved representation of subdominant community dynamics in ecosystem models and remote‐sensing algorithms.

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Section: Methodsmentioning

confidence: 99%

The role of differential DMSP production and community composition in predicting variability of global surface DMSP concentrations

McParland

Levine

2018

Limnology & Oceanography

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“…Samples were analysed at high flow rate (∼ 70 µL min −1 ), and specific phytoplankton groups were discriminated in bivariate scatter plots by differences in side scatter and red-orange fluorescence (Tarran et al, 2001). In order to identify the phytoplankton responsible for DMSP production in the mesocosms, fluorescence activated cell sorting was used to determine group-specific intracellular DMSP content (Archer et al, 2011). This was carried out using water sampled from two contrasting mesocosms on two separate occasions towards the end of the experiment (t18 and t21).…”

Section: Flow Cytometry and Group-specific Dmsp Cell Contentmentioning

confidence: 99%

Contrasting responses of DMS and DMSP to ocean acidification in Arctic waters

et al. 2013

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Abstract. Increasing atmospheric CO2 is decreasing ocean pH most rapidly in colder regions such as the Arctic. As a component of the EPOCA (European Project on Ocean Acidification) pelagic mesocosm experiment off Spitzbergen in 2010, we examined the consequences of decreased pH and increased pCO2 on the concentrations of dimethylsulphide (DMS). DMS is an important reactant and contributor to aerosol formation and growth in the Arctic troposphere. In the nine mesocosms with initial pHT 8.3 to 7.5, equivalent to pCO2 of 180 to 1420 μatm, highly significant but inverse responses to acidity (hydrogen ion concentration [H&plus;]) occurred following nutrient addition. Compared to ambient [H&plus;], average concentrations of DMS during the mid-phase of the 30 d experiment, when the influence of altered acidity was unambiguous, were reduced by approximately 60% at the highest [H&plus;] and by 35% at [H&plus;] equivalent to 750 μatm pCO2, as projected for 2100. In contrast, concentrations of dimethylsulphoniopropionate (DMSP), the precursor of DMS, were elevated by approximately 50% at the highest [H&plus;] and by 30% at [H&plus;] corresponding to 750 μatm pCO2. Measurements of the specific rate of synthesis of DMSP by phytoplankton indicate increased production at high [H&plus;], in parallel to rates of inorganic carbon fixation. The elevated DMSP production at high [H&plus;] was largely a consequence of increased dinoflagellate biomass and in particular, the increased abundance of the species Heterocapsa rotundata. We discuss both phytoplankton and bacterial processes that may explain the reduced ratios of DMS:DMSPt (total dimethylsulphoniopropionate) at higher [H&plus;]. The experimental design of eight treatment levels provides comparatively robust empirical relationships of DMS and DMSP concentration, DMSP production and dinoflagellate biomass versus [H&plus;] in Arctic waters.

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“…Previous work has used sorting flow cytometry to elucidate cellular DMSP production in groups of phytoplankton functional types (PFTs) in natural samples from the Mauritanian Upwelling in the eastern Atlantic Ocean [22]. However, the approach of Archer et al [22] did not elucidate the contribution that individual species made in situ to the cellular DMSP production within each PFT class.…”

Section: Introductionmentioning

confidence: 97%

“…However, the approach of Archer et al [22] did not elucidate the contribution that individual species made in situ to the cellular DMSP production within each PFT class. Relationships of DMSP per cell in cytometrically-sorted PFT classes ignore taxon-specific cellular quotas of DMSP production, making it difficult to extrapolate any of the size class relationships to other regions of the world's oceans where species composition may differ within each PFT.…”

Section: Introductionmentioning

confidence: 98%

“…A number of environmental conditions have also been shown to change intraspecific cellular DMSP concentrations, including temperature, salinity, light, and in some taxa nutrient limitation [8,17À21]. The differences in phytoplankton DMSP production under changing environmental conditions have been well established in the laboratory, but taxon-specific field measurements are rare [22]. This limitation has the potential to introduce bias into models when extrapolating laboratory-based cellular quotas to the environment due to, among other things, the nutrient enriched conditions of culture media and the age of the cultures.…”

Section: Introductionmentioning

confidence: 99%

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In situdetermination of cellular DMSP and pigment quotas in aProrocentrum minimumbloom near the Falkland Islands

Cyronak

O’Reilly

Lee

et al. 2014

Advances in Oceanography and Limnology

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Marine phytoplankton play critical roles in the biogeochemistry of open and coastal oceans. However, the impact that individual species have on an ecosystem-wide scale can strongly depend on the production of cellular compounds, especially those that are climatically active such as dimethylsulfide (DMS). Herein, we use sorting flow cytometry to separate a distinct phytoplankton population from four samples taken along the Patagonian shelf near the Falkland Islands. Morphological, genetic, and biochemical analyses demonstrated that three of the sorted samples were dominated by a bloom of the dinoflagellate Prorocentrum minimum. Cellular quotas of the DMSprecursor dimethylsulfoniopropionate (DMSP) ranged from 1.23À4.11 pg cell ¡1 in the same population at different sampling stations. Causes of this variability may be due to different growth stages of the P. minimum bloom or changes in other environmental variables. Overall, in situ intracellular DMSP concentrations were lower than what would be expected based on previous, culture-based measurements. We demonstrate the difficulties inherent in sorting individual phytoplankton species from natural samples in order to determine in situ species-specific cellular quotas of important biogeochemical compounds.

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Combining cell sorting with gas chromatography to determine phytoplankton group-specific intracellular dimethylsulphoniopropionate

Cited by 22 publications

References 38 publications

The role of differential DMSP production and community composition in predicting variability of global surface DMSP concentrations

The role of differential DMSP production and community composition in predicting variability of global surface DMSP concentrations

Contrasting responses of DMS and DMSP to ocean acidification in Arctic waters

In situdetermination of cellular DMSP and pigment quotas in aProrocentrum minimumbloom near the Falkland Islands

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