Combining multiple imaging techniques at the TwinMic X-ray microscopy beamline

Gianoncelli, Alessandra; Kourousias, George; Altissimo, Matteo; Bedolla, Diana E.; Merolle, Lucia; Stolfa, Andrea; Shin, Hyun‐Joon

doi:10.1063/1.4961136

Cited by 17 publications

(9 citation statements)

References 14 publications

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“…The flattened sections were freeze-dried and mounted onto gold folding grids for analysis with the TwinMic spectromicroscope. 22 The brightfield and differential phase contrast images were acquired at 1.95 keV in scanning transmission mode 23 with a spot size of 1.2 µm in diameter. Images were supplemented with LEXRF microscopy emission maps of C, O, O/C ratio, Al, Si, and other trace elements (e.g.…”

Section: Methodsmentioning

confidence: 99%

Treatment of osteoporosis with a modified zeolite shows beneficial effects in an osteoporotic rat model and a human clinical trial

Pavelić

Micek

Bobinac

et al. 2020

Exp Biol Med (Maywood)

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The severity of osteoporosis in humans manifests in its high incidence and by its complications that diminish quality of life. A societal consequence of osteoporosis is the substantial burden that it inflicts upon patients and their families. Several bone-modifying drugs have been prescribed to patients with osteoporosis. However, evidence for their anti-fracture efficacy remains inconclusive. To the contrary, long-term use of anti-osteoporotic drugs such as bisphosphonates and Denosumab, an RANKL inhibitor, have resulted in adverse events. We now present an alternative and adjuvant approach for treatment of osteoporosis. The data derive from in vivo studies in an ovariectomized rat model and from a randomized double blind, placebo-controlled human clinical study. Both studies involved treatment with Panaceo Micro Activation (PMA)-zeolite-clinoptilolite, a defined cation exchange clinoptilolite, which clearly improved all bone histomorphometric parameters examined from ovariectomized animals, indicative for increased bone formation. Moreover, intervention with PMA-zeolite-clinoptilolite for one year proved safe in humans. Furthermore, patients treated with PMA-zeolite-clinoptilolite showed an increase in bone mineral density, an elevated level of markers indicative of bone formation, a significant reduction in pain, and significantly improved quality of life compared with patients in the control (placebo) group. These encouraging positive effects of PMA-zeolite-clinoptilolite on bone integrity and on osteoporosis warrant further evaluation of treatment with PMA-zeolite-clinoptilolite as a new alternative adjuvant therapy for osteoporosis.

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Section: Methodsmentioning

confidence: 99%

Treatment of osteoporosis with a modified zeolite shows beneficial effects in an osteoporotic rat model and a human clinical trial

Pavelić

Micek

Bobinac

et al. 2020

Exp Biol Med (Maywood)

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“…Most of the experiments were performed at the TwinMic beamline 46 of the ELETTRA synchrotron facility (ELETTRA, Trieste, Italy, www.elettra.trieste.it/twinmic ) using the scanning X-ray microscopy (SXM) mode. In the SXM configuration the sample is raster-scanned with respect to a microprobe generated by Zone plate focusing optics.…”

Section: Methodsmentioning

confidence: 99%

Iron-related toxicity of single-walled carbon nanotubes and crocidolite fibres in human mesothelial cells investigated by Synchrotron XRF microscopy

Cammisuli

Giordani

Gianoncelli

et al. 2018

Sci Rep

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Carbon nanotubes (CNTs) are promising products in industry and medicine, but there are several human health concerns since their fibrous structure resembles asbestos. The presence of transition metals, mainly iron, in the fibres seems also implicated in the pathogenetic mechanisms. To unravel the role of iron at mesothelial level, we compared the chemical changes induced in MeT-5A cells by the exposure to asbestos (crocidolite) or CNTs at different content of iron impurities (raw-SWCNTs, purified- and highly purified-SWCNTs). We applied synchrotron-based X-Ray Fluorescence (XRF) microscopy and soft X-ray imaging (absorption and phase contrast images) to monitor chemical and morphological changes of the exposed cells. In parallel, we performed a ferritin assay. X-ray microscopy imaging and XRF well localize the crocidolite fibres interacting with cells, as well as the damage-related morphological changes. Differently, CNTs presence could be only partially evinced by low energy XRF through carbon distribution and sometimes iron co-localisation. Compared to controls, the cells treated with raw-SWCNTs and crocidolite fibres showed a severe alteration of iron distribution and content, with concomitant stimulation of ferritin production. Interestingly, highly purified nanotubes did not altered iron metabolism. The data provide new insights for possible CNTs effects at mesothelial/pleural level in humans.

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“…The testing of DANTE has been carried out at TwinMic soft X‐ray microscopy synchrotron beamline (Elettra–Sincrotrone Trieste, Trieste, Italy). TwinMic was operated in Scanning mode, where a 600‐μm diameter and 50‐nm outermost zone width Zone Plate was used to focus a monochromatic 1 keV photon beam on the sample plane. The Zone Plate imaged a 75μm circular secondary source into a roughly 850‐nm diameter spot size at the sample position.…”

Section: Methodsmentioning

confidence: 99%

“…In this paper, we discuss the performances of the DANTE digital pulse processor (DPP), which was tested at 1 keV at the TwinMic soft X‐ray microscopy beamline (Elettra Sincrotrone Trieste, Trieste, Italy). The current photon flux and the available solid angle coverage of the TwinMic XRF system do not allow for fast chemical imaging .…”

Section: Introductionmentioning

confidence: 99%

Towards on‐the‐fly X‐ray fluorescence mapping in the soft X‐ray regime

Bombelli¹,

Manotti²,

Altissimo

et al. 2018

X-Ray Spectrometry

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The possibility of fast X‐ray fluorescence (XRF) imaging using on‐the‐fly acquisition is very attractive due to its high acquisition speed. The underlying assumption is that there must be a meaningful flux of XRF emitted photons collected by the detectors. On‐the‐fly XRF has already been demonstrated at mid‐to‐high X‐ray energies, both for laboratory and synchrotron‐based instruments. In this manuscript, we report on the capability of DANTE digital pulse processor (DPP) in the soft X‐ray regime. DANTE is a novel compact and low‐power DPP with high energy resolution and high count rate. This work presents preliminary measurements performed at the TwinMic beamline at Elettra synchrotron (Trieste, Italy) addressing the feasibility of on‐the‐fly low energy XRF imaging.

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Combining multiple imaging techniques at the TwinMic X-ray microscopy beamline

Cited by 17 publications

References 14 publications

Treatment of osteoporosis with a modified zeolite shows beneficial effects in an osteoporotic rat model and a human clinical trial

Treatment of osteoporosis with a modified zeolite shows beneficial effects in an osteoporotic rat model and a human clinical trial

Iron-related toxicity of single-walled carbon nanotubes and crocidolite fibres in human mesothelial cells investigated by Synchrotron XRF microscopy

Towards on‐the‐fly X‐ray fluorescence mapping in the soft X‐ray regime

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