Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels

Abstract

The immune and antioxidant genetic factors that could converse with mastitis susceptibility in dromedary camels were looked at in this research. Of 120 female dromedary camels (60 healthy, and 60 with mastitis) were utilised. Each camel’s jugular vein was pierced to obtain five millilitres of blood. The blood was placed within tubes containing sodium fluoride or EDTA anticoagulants to obtain whole blood and extract DNA and RNA. The immunological (OTUD3, TLR2, TLR4, STAB2, MBL2, TRAPPC9, and C4A) and antioxidant (CAT, SOD3, PRDX6, OXSR1, NDUFS6, SERP2, and ST1P1) genes’ nucleotide sequence polymorphisms between healthy and mastitis affected she-camels were discovered using PCR-DNA sequencing. Fisher’s exact test revealed that camel groups with and without mastitis had noticeably different odds of all major nucleotide alterations propagating (p < 0.01). Mastitic camels were significantly more likely to express the OTUD3, TLR2, TLR4, STAB2, MBL2, TRAPPC9, C4A, OXSR1, SERP2, and ST1P1 genes (p < 0.05). However, CAT, SOD3, PRDX6, and NDUFS6 genes elicited a different pattern. The results may be used to develop management strategies and support the significance of nucleotide differences and gene expression patterns in these markers as indicators of the incidence of mastitis.

Gene Expression of Oxidative/Antioxidative Markers, VDR, CAMK4 and Ceruloplasmin in Baladi Sheep a with Minerals Deficiency

“…Oxidative stress-responsive kinase 1 (OXSR1) gene encodes OSR1, which controls downstream kinases in response to environmental stressors [54]. OXSR1 gene was up-regulated in endometritis Holstein cattle [55] and mastitic dromedary camels [56]. The observed alterations in the expression pattern of antioxidant and OXSR1 expression may be explained by a rise in oxidative damage resulting from a decrease in the levels of Ca and Cu, which in turn causes a decrease in SOD, CAT, and GPX [57]; [58].…”

New Insights into Polymorphisms in Candidate Genes Associated with Incidence of Postparturient Endometritis in Ossimi Sheep (Ovis aries)

“…In order to fully eradicate diseased animals or utilize disease-resistant livestock, it is imperative to comprehend the underlying mutations and interactions that lead to genetic resistance [32]. Sequencing of the amplified PCR products revealed variations in the immunological, metabolic and antioxidant genes in both endometritis and healthy Ossimi ewes.…”