Combining passive samplers and biomonitors to evaluate endocrine disrupting compounds in a wastewater treatment plant by LC/MS/MS and bioassay analyses

h i g h l i g h t sWe evaluate effect of E1 on the early life stage of Japanese medaka. EI affected hatchability, time to hatching and the growth, deteriorated development. E1 changed ERa and Vtg-I gene mRNA levels of both sexes at low concentrations. E1 has unrecoverable impacts on medaka, even if only early life stages were exposed. a r t i c l e i n f o b s t r a c tThe fertilized eggs of Japanese medaka (Oryzias latipes) were exposed to estrone (E1) at 5-5000 ng L À1 for 15 d, and the hatched fry were exposed continuously to the same concentrations for the additional 15 d. Adverse effects on hatchability, time to hatching, and gross abnormalities occurred at 50 ng L À1 or above.Then the fry were divided into a continual exposure group, and a water recovery group. When the fry were exposed to E1 for another 60 d, there was a decrease in the hepatosomatic index (HSI) of males and the influence disappeared in the water recovery group. The gonadosonatic index (GSI) of females at 500 ng L À1decreased significantly in another 60 d exposure. While the fry were maintained in dechlorinated tap water for 60 d, a significant decrease in female GSI was observed at 50 ng L À1 or above. An increased GSI was found in males in both continual exposure and water recovery groups at all E1 treatments. Quantitative RT-PCR showed that vitellogenin-I (Vtg-I) gene expressions in the female liver were significantly down-regulated at 50 ng L À1 in the continual exposure group, and at 500 ng L À1 in the water recovery group, while male Vtg-I genes were significantly up-regulated for all E1 treatments. In addition, all E1 treatments caused sex reversal of males. These results suggest that E1 at 5 ng L À1 or above have unrecoverable impacts on the gonadal growth and development of medaka, even if only early life stages were exposed to E1.

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“…E1 has a lipophilicity log k ow estimated to be 3.1 (Liscio et al, 2009) which can partition into lipid compartments (e.g. membranes).…”

Section: Discussionmentioning

confidence: 99%

Effects of estrone on the early life stages and expression of vitellogenin and estrogen receptor genes of Japanese medaka (Oryzias latipes)

et al. 2013

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“…Arrainek, ordea, konposatu horiek metatzeko gaitasuna dute, eta hori dela bide, kontzentrazio-maila altuagoetan aurki daitezke. Beraz, ikertzaile askok arrainak eta muskuiluak erabili dituzte biomonitorizazio-tresnatzat [69,70].…”

Section: Konposatu Kimikoen Metaketaunclassified

Konposatu disruptore endokrinoen azterketa kimika analitikoaren ikuspuntutik

Ros¹,

Vallejo²,

Olivares³

et al. 2018

EKAIA

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Laburpena: Ingurumeneko uretan kutsatzaile organiko desberdinak aurkitzen dira, nahiz eta kontzentrazio baxuetan izan (ng/L mailan). Araztegien irteera korronteak izan ohi dira konposatu horien iturririk nagusietako bat, araztegiek erabiltzen dituzten mekanismoak ez baitira gai kutsatzaileen arazketa osoa burutzeko. Horien artean, konposatu disruptore endokrino (EDC) izenez ezagutzen diren konposatuak aurki daitezke, eta horiek ingurumenean bizi diren espezieetan aldaketa endokrinoak sor ditzakete, nahiz eta EDCak kontzentrazio baxuetan egon. Kalte horien artean, malformazioak, populazioen aldaketak edo intersexualitatea dira nabarmenenak. Hau guztia ikusirik, kimika analitikoaren ikuspuntutik oso garrantzitsua da metodo analitiko sentikorrak garatzea konposatuak ur-eta biota-laginetatik erauzi eta garbitzeko, bai eta konposatuek biotaren atal desberdinetan metatzeko duten joera aztertzeko ere.Hitz gakoak: konposatu disruptore endokrinoak, erauzketa-metodoak, biokontzentrazioa, ur-laginak, biota.Abstract: There are several organic contaminants present in the aquatic environment, even they are find at very low concentrations (ng/L level). The effluents of the wastewater treatment plants (WWTP) can be one of the main sources into the environment, since the mechanism used by the WWTP are not fully efective for the complete removal of the contaminants. Among them, some of them are classified as endocrine disrupting compounds (EDCs) and they can provoke endocrine alterations in the species habiting in the water even they are found at low concentrations. Malformations, a decrease on the polulation or intersexuality are the most significant damages. Thus, the development of highly sensitive analytical methods for the extraction and clean-up of the water and biota samples is vital from an analytical point of view together with the accumulation capacity of the compounds in the different biota compartments.

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“…In risk assessment, by comparing EEQs obtained from both analytical determination and bioassay, the major and minor contributors of a complex sample could be identified. When this was applied to environmental waters, some work reported that the predicted overall EEQs were similar to observed ones (Beck et al, 2006;Jiang et al, 2012;Liscio et al, 2009), but some found that the predictions were higher or lower (Cargouët et al, 2004;Furuichi et al, 2004). These contradictory conclusions may result from the contribution of non-targets chemicals such as unknown estrogen agonist, antagonist and humics, or the multiple involved modes of action (MoAs) and the resulting interaction of mixture components (Villeneuve et al, 2000).…”

Section: Introductionmentioning

confidence: 98%

Combined effects of estrogenic chemicals with the same mode of action using an estrogen receptor binding bioassay

Yang

et al. 2014

Environmental Toxicology and Pharmacology

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Combining passive samplers and biomonitors to evaluate endocrine disrupting compounds in a wastewater treatment plant by LC/MS/MS and bioassay analyses

Cited by 63 publications

References 26 publications

Effects of estrone on the early life stages and expression of vitellogenin and estrogen receptor genes of Japanese medaka (Oryzias latipes)

Effects of estrone on the early life stages and expression of vitellogenin and estrogen receptor genes of Japanese medaka (Oryzias latipes)

Konposatu disruptore endokrinoen azterketa kimika analitikoaren ikuspuntutik

Combined effects of estrogenic chemicals with the same mode of action using an estrogen receptor binding bioassay

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