Combining Physio-Biochemical Characterization and Transcriptome Analysis Reveal the Responses to Varying Degrees of Drought Stress in Brassica napus L.

Fang, Shuai; Zhao, Peimin; Tan, Zengdong; Peng, Yan; Xu, Lintang; Jin, Yutong; Wei, Fang; Guo, Liang; Yao, Xuan

doi:10.3390/ijms23158555

Cited by 9 publications

(5 citation statements)

References 61 publications

(62 reference statements)

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“…Rapeseed is one of the most important seed oil‐bearing crops, but drought stress severely influences its growth and yield (Fang et al, 2022). Drought stress can induce ABA and proline accumulation (Cheng et al, 2022; Ren et al, 2018), and thereby induce a series of responses, such as stomatal movement (Liu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

RING‐type E3 ligase BnaJUL1 ubiquitinates and degrades BnaTBCC1 to regulate drought tolerance in Brassica napus L

Hu,

Luo,

Zhou

et al. 2023

Plant Cell & Environment

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Drought stress poses a persistent threat to field crops and significantly limits global agricultural productivity. Plants employ ubiquitin‐dependent degradation as a crucial post‐translational regulatory mechanism to swiftly adapt to changing environmental conditions. JUL1 is a RING‐type E3 ligase related to drought stress in Arabidopsis. In this study, we explored the function of BnaJUL1 (a homologous gene of JUL1 in Brassica napus) and discovered a novel gene BnaTBCC1 participating in drought tolerance. First, we utilised BnaJUL1‐cri materials through the clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated protein 9 system. Second, we confirmed that BnaJUL1 regulated drought tolerance through the drought tolerance assay and transcriptome analysis. Then, we identified a series of proteins interacting with BnaJUL1 through yeast library screening, including BnaTBCC1 (a tubulin binding cofactor C domain‐containing protein); whose homologous gene TBCC1 knockdown mutants (tbcc1‐1) exhibited ABA‐sensitive germination in Arabidopsis, we then confirmed the involvement of BnaTBCC1 in drought tolerance in both Arabidopsis and Brassica. Finally, we established that BnaJUL1 could ubiquitinate and degrade BnaTBCC1 to regulate drought tolerance. Consequently, our study unveils BnaJUL1 as a novel regulator that ubiquitinates and degrades BnaTBCC1 to modulate drought tolerance and provided desirable germplasm for further breeding of drought tolerance in rapeseed.

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Section: Discussionmentioning

confidence: 99%

RING‐type E3 ligase BnaJUL1 ubiquitinates and degrades BnaTBCC1 to regulate drought tolerance in Brassica napus L

Hu,

Luo,

Zhou

et al. 2023

Plant Cell & Environment

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“…Transcriptomic analysis of another representative of Brassicaceae-rapeseed (Brassica napus) revealed that most significantly enriched GO terms of the upregulated DEGs were related with the response to water deprivation, ABA signaling, osmotic stress, and other abiotic stimuli and lipid metabolism as well as cutin, suberin, and wax biogenesis, fatty acid degradation and secondary compound metabolism. On the contrary, downregulated DEGs were connected mainly with photosynthetic activities, porphyrin metabolism, carbon, and nitrogen metabolism [110].…”

Section: Water Deficiency (Drought)mentioning

confidence: 94%

Plant Transcriptomic Responses in a Diverse Organ and Stress Conditions

Rurek,

Smolibowski

2024

Preprint

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Plant transcriptomes are an extremely dynamic entities shaped spatially and temporally by many intracellular and environmental cues. In this review, we first summarize the complexity and diversity of plant genomes and transcriptomes as a start point for the multitude of transcriptomic responses. Numerous alterations within various tissue and organ-specific transcriptomes as well as the most relevant transcriptomic responses associated with plant acclimation to selected abiotic and biotic stress conditions, from the current studies employing high-throughput transcriptomic analysis are widely discussed. Understanding changes within plant transcriptomes, revealed by in silico functional analysis, allows for the characterization of stress-affected genes and stress acclimatory mechanisms, as well as allows to perform plant metabolic engineering. The latter allow cultivars to produce more secondary metabolites in the future, which are often desirable substances in the biomedical industry. Accordingly, in this review special attention was also paid to characterize the potential of transcriptomic analyses of medicinal species, particularly to search for new cultivars. Extensive characterization of transcriptomic responses in stress would also result in the development of new cultivars that display physiological and molecular mechanisms that allow them to cope with adverse environmental conditions more adequately.

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“…There were 795 genes up‐regulated and 1,050 genes down‐regulated under severe drought stress. Among them, overexpression of calcineurin B‐like protein (CBL)‐interacting protein kinase ( BnA01.CIPK6 ) was found to improve drought tolerance (Fang et al, 2022). With the rise of phenomics, drought‐related phenotypes can be accessed more precisely in the future to aid in the identification of drought resistance genes in B. napus .…”

Section: Abiotic Stressmentioning

confidence: 99%

Functional genomics of Brassica napus: Progress, challenges, and perspectives

Tan,

Han,

Dai

et al. 2024

JIPB

Self Cite

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Brassica napus, commonly known as rapeseed or canola, is a major oil crop contributing over 13% to the stable supply of edible vegetable oil worldwide. Identification and understanding the gene functions in the B. napus genome is crucial for genomic breeding. A group of genes controlling agronomic traits have been successfully cloned through functional genomics studies in B. napus. In this review, we present an overview of the progress made in the functional genomics of B. napus, including the availability of germplasm resources, omics databases and cloned functional genes. Based on the current progress, we also highlight the main challenges and perspectives in this field. The advances in the functional genomics of B. napus contribute to a better understanding of the genetic basis underlying the complex agronomic traits in B. napus and will expedite the breeding of high quality, high resistance and high yield in B. napus varieties.

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Combining Physio-Biochemical Characterization and Transcriptome Analysis Reveal the Responses to Varying Degrees of Drought Stress in Brassica napus L.

Cited by 9 publications

References 61 publications

RING‐type E3 ligase BnaJUL1 ubiquitinates and degrades BnaTBCC1 to regulate drought tolerance in Brassica napus L

RING‐type E3 ligase BnaJUL1 ubiquitinates and degrades BnaTBCC1 to regulate drought tolerance in Brassica napus L

Plant Transcriptomic Responses in a Diverse Organ and Stress Conditions

Functional genomics of Brassica napus: Progress, challenges, and perspectives

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