Combining Prebiotic and Mixture of Freeze Dried Probiotics to Develop Synbiotics that Induce Apoptosis in Human Colon Tumor Cells

Abstract

IN THE PRESENT study, we first aimed to select some potential anti-cancer probiotics in vitro. Using the inulin fermentation product by the selected five probiotic bacteria (Lactobacillus paraplantarum strain S143, L. pentosus strain P2-3, L. plantarum strain J12, L.gasseri strain F. and L.reuteri strain IRT), Caco-2 cells were exposed to 50% filtrate supernatant of each probiotic bacteria after 24 h of incubation. Results showed that inulin fermentation product of the selected probiotics cause apoptosis in the Caco-2 cell line. Also, the viability of the freezedried powders of the five probiotics using skimmed milk as cryoprotectant was followed up for 12 weeks of storage at refrigerated conditions and also at room temperature. Results proved that lyophilized powders showed slight decrease in their viable count during storage both at room and refrigerated temperatures. This would ensure a good cell concentration for at least 3 months to be administered as a probiotic and would guarantee the long-term delivery of sufficient active, viable, and functional cultures.

Bio-protective Effects of Lactobacillus Plantarum subsp.plantarum Against Aflatoxin B1 Genotoxicity on Human Blood Lymphocytes: A Native Probiotic Strain Isolated FromIranian Camel Milk

“…Combined treatment of AFB1 with two final doses of viable bacteria 10 9 and 10 11 CFU/mL did not lead to a significant difference regarding the inhibition or reduction of lymphocyte damage (Figure 4D). As the presence of a suitable amount, at least 108-10 9 CFU of viable cells, in the probiotic formulation is a vital criterion for health claims [ 35 ]. Consequently, based on the results of the present study, it seems that the sufficient dose for prophylactic potential versus AFB1 genotoxicity is 10 9 CFU, and the increase of bacterial cell to 10 11 CFU/mL amount or more probably has therapeutic effects.…”

The potential of Lactobacillus buchneri isolated from spontaneous rabbit meat fermented - bekasam against Salmonella typhimurium by in vivo evaluation

“…The feed was offered orally according to treatments for seven days using a method by Mohamed et al (2010) and Giles-Gómez et al (2016). On the 8 th days, the mice were infected with S. typhimurium (0.2 mL containing 1×10 7 CFU/mL dissolved in physiological NaCl).…”