Combining quantitative trait loci and heterogeneous microarray data analyses reveals putative candidate pathways affecting mastitis in cattle

Lewandowska-Sabat, Anna; Günther, Juliane; Seyfert, Hans‐Martin; Olsaker, I.

doi:10.1111/j.1365-2052.2012.02342.x

Cited by 14 publications

(14 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A subset of the differentially regulated genes (List eQG) with genomic positions linked to quantitative trait loci (QTL) affecting susceptibility to mastitis in Norwegian Red cattle (NRF) was identified previously [18]. Twenty-five of the List eQG genes were mapped to 5 different KEGG pathways ( p ≤ 0.05), mainly signaling in immune response and cancer (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…A) The list of differentially regulated on microarray (n = 418) and Ensembl reannotated genes was used as an input for functional annotation clustering; B) Subset of these genes (n = 28; List eQG from [18]) was used as an input. Asterisk (*) denotes the significant KEGG pathways after the Benjamini–Hochberg multiple testing correction ( p ≤ 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…A) The list of differentially regulated on microarray (n = 418) and Ensembl reannotated genes was used as an input for IPA; B) Subset of these genes (n = 28; List eQG from [18]) was used as an input.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

The early phase transcriptome of bovine monocyte-derived macrophages infected with Staphylococcus aureus in vitro

et al. 2013

View full text Add to dashboard Cite

BackgroundIn the mammary gland, local recruitment and action of macrophages is a key immunological defence mechanism against infection. Macrophages are members of the innate immune system, serve as the first line of the defence against invading pathogens and are critical effectors and regulators of inflammation. We have examined the early phase response of bovine macrophages to infection with live Staphylococcus aureus. Genome-wide transcript profiling of blood monocyte-derived macrophages from six Norwegian Red heifers infected with live S. aureus for 2 and 6 hours in vitro was performed.ResultsAbout 420 of the 17 000 genes on the ARK-Genomics bovine cDNA array were differentially regulated at 6 hours post infection. Approximately 70% of the responding genes had a known identity (Entrez Gene ID) and were used in the identification of overrepresented pathways and biological functions in the dataset.Analysis of a subset of differentially regulated genes (List eQG) obtained by comparison with data from genome-wide association mapping in Norwegian Red cattle identified anti-inflammatory cytokines interleukin 4 and interleukin 13 as putative expression quantitative trait loci, suggesting that S. aureus infection triggers alternative activation of macrophages. Moreover, several classical activation pathways were found, mainly cellular immune response and cytokine signaling pathways, i.e. triggering receptor expressed on myeloid cells 1 (TREM1) and nucleotide-binding and oligomerization domain-like receptor (NLR) pathways. Tumor necrosis factor receptor superfamily member 5 (CD40 ligand) was identified as an upstream regulator which points toward CD40 likely acting as a co-stimulatory receptor during Toll-like receptor 2(TLR2)-mediated inflammatory response of bovine macrophages to S. aureus infection. Furthermore, peptidoglycan was identified as an upstream regulator in the List eQG, which indicates that this bacterial cell-wall component might be pivotal in macrophage intracellular bacterial recognition during early inflammation.ConclusionsHere we have shown that in vitro infection of bovine macrophages with live S. aureus induced both alternative and classical activation pathways. Alternative activation of macrophages may be a mechanism contributing to intracellular persistence of S. aureus in the course of inflammation such as during mastitis in dairy cattle.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The early phase transcriptome of bovine monocyte-derived macrophages infected with Staphylococcus aureus in vitro

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In cattle, various GWAS have been performed to evaluate genetic differences for a variety of traits like production, reproduction and conformation [13] and for susceptibility or resistance to certain disease such as Johne’s disease [14, 15], bovine tuberculosis [16], mastitis or somatic cell score [17–19]. These studies have been useful for identifying SNP markers and genes associated with a particular disease [20, 21].…”

Section: Introductionmentioning

confidence: 99%

A genome-wide association study of immune response traits in Canadian Holstein cattle

et al. 2014

View full text Add to dashboard Cite

BackgroundBreeding for enhanced immune response (IR) has been suggested as a tool to improve inherent animal health. Dairy cows with superior antibody-mediated (AMIR) and cell-mediated immune responses (CMIR) have been demonstrated to have a lower occurrence of many diseases including mastitis. Adaptive immune response traits are heritable, and it is, therefore, possible to breed for improved IR, decreasing the occurrence of disease. The objective of this study was to perform genome-wide association studies to determine differences in genetic profiles among Holstein cows classified as High or Low for AMIR and CMIR. From a total of 680 cows with immune response phenotypes, 163 cows for AMIR (81 High and 82 Low) and 140 for CMIR (75 High and 65 Low) were selectively genotyped using the Illumina Bovine SNP50 BeadChip. Results were validated using an unrelated population of 164 Holstein bulls IR phenotyped for AMIR and 146 for CMIR.ResultsA generalized quasi likelihood score method was used to determine single nucleotide polymorphisms (SNP) and chromosomal regions associated with immune response. After applying a 5% chromosomal false discovery rate, 186 SNPs were significantly associated with AMIR. The majority (93%) of significant markers were on chromosome 23, with a similar peak found in the bull population. For CMIR, 21 SNP markers remained significant. Candidate genes within 250,000 base pairs of significant SNPs were identified to determine biological pathways associated with AMIR and CMIR. Various pathways were identified, including the antigen processing and presentation pathway, important in host defense. Candidate genes included those within the bovine Major Histocompatability Complex such as BoLA-DQ, BoLA-DR and the non-classical BoLA-NC1 for AMIR and BoLA-DQ for CMIR, the complement system including C2 and C4 for AMIR and C1q for CMIR, and cytokines including IL-17A, IL17F for AMIR and IL-17RA for CMIR and tumor necrosis factor for both AMIR and CMIR. Additional genes associated with CMIR included galectins 1, 2 and 3, BCL2 and β-defensin.ConclusionsThe significant genetic variation associated with AMIR and CMIR in this study may imply feasibility to include immune response in genomic breeding indices as an approach to improve inherent animal health.

show abstract

“…Several candidate pathways were also found in the study on integrated genomic data from genome-wide association mapping in cattle, and transcriptomic data from microarray studies on mastitis pathogens. Of great interest are and signaling pathways [3].…”

Section: Introductionmentioning

confidence: 99%

Sequencing and phylogenetic analysis of partial CXCR2 gene of Murrah buffalo

Wani¹,

Sangwan²,

Dar³

et al. 2014

Vet World

View full text Add to dashboard Cite

Combining quantitative trait loci and heterogeneous microarray data analyses reveals putative candidate pathways affecting mastitis in cattle

Cited by 14 publications

References 33 publications

The early phase transcriptome of bovine monocyte-derived macrophages infected with Staphylococcus aureus in vitro

The early phase transcriptome of bovine monocyte-derived macrophages infected with Staphylococcus aureus in vitro

A genome-wide association study of immune response traits in Canadian Holstein cattle

Sequencing and phylogenetic analysis of partial CXCR2 gene of Murrah buffalo

Contact Info

Product

Resources

About