Combining the targeted and untargeted screening of environmental contaminants reveals associations between PFAS exposure and vitamin D metabolism in human plasma

Carlsson, Henrik; Sreenivasan, Akshai P; Erngren, Ida; Larsson, Anders; Kultima, Kim

doi:10.1039/d3em00060e

Cited by 2 publications

(2 citation statements)

References 67 publications

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“…It can be seen in Figure 1 that none of the PFAAs correlated with either 25(OH)D 3 or 1,25(OH) 2 D 3 : PFOS ( Figure 1 A,B), PFOA ( Figure 1 C,D), PFHxS ( Figure 1 E,F), and PFNA ( Figure 1 G,H). When PFOS, PFOA, PFNA, and PFHxS were correlated as a combined group for vitamin sufficiency (>20 ng/mL) and vitamin D deficiency (<20 ng/mL) [ 41 ], still no correlation with PFOS, PFOA, PFHxS, or PFNA was found. It can be seen in Figure 2 that none of the PFAAs correlated with either sufficient or deficient 25(OH)D 3 : PFOS ( Figure 2 A,B), PFOA ( Figure 2 C,D), PFHxS ( Figure 2 E,F), and PFNA ( Figure 2 G,H).…”

Section: Resultsmentioning

confidence: 99%

“…Recent reports indicate that PFAA exposure is associated with cardiovascular disease, diabetes, and vitamin D deficiency, but studies in humans have provided contradictory results that require further evaluation of the biological toxicity of PFAAs [ 39 ]. Further studies have reported that, in pregnant African American women, PFAA exposure may affect vitamin D levels [ 40 ] and that perfluorohexane sulfonate (PFHxS) correlates strongly with vitamin D 3 metabolites [ 41 ]. In silico, PFOA bound competitively to the Vitamin D Receptor (VDR), and, indeed, mixtures of PFAAs may also activate the VDR, which has a critical role in the regulation of genes important for the maintenance of calcium homeostasis and immune and endocrine functions [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

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Association of Vitamin D with Perfluorinated Alkyl Acids in Women with and without Non-Obese Polycystic Ovary Syndrome

Butler,

Sathyapalan,

Das

et al. 2024

Biomedicines

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Background. Perfluorinated alkyl acids (PFAAs) are persistent organic pollutants affected by BMI and ethnicity, with contradictory reports of association with vitamin D deficiency. Methods. Twenty-nine Caucasian women with non-obese polycystic ovary syndrome (PCOS) and age- and BMI-matched Caucasian control women (n = 30) were recruited. Paired serum samples were analyzed for PFAAs (n = 13) using high-performance liquid chromatography–tandem mass spectrometry. Tandem mass spectrometry determined levels of 25(OH)D3 and the active 1,25(OH)2D3. Results. Women with and without PCOS did not differ in age, weight, insulin resistance, or systemic inflammation (C-reactive protein did not differ), but the free androgen index was increased. Four PFAAs were detected in all serum samples: perfluorooctane sulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorohexane sulfonic acid (PFHxS). Serum PFOS was higher in PCOS versus controls (geometric mean [GM] 3.9 vs. 3.1 ng/mL, p < 0.05). Linear regression modeling showed that elevated PFHxS had higher odds of a lower 25(OH)D3 (OR: 2.919, 95% CI 0.82–5.75, p = 0.04). Vitamin D did not differ between cohorts and did not correlate with any PFAAs, either alone or when the groups were combined. When vitamin D was stratified into sufficiency (>20 ng/mL) and deficiency (<20 ng/mL), no correlation with any PFAAs was seen. Conclusions. While the analyses and findings here are exploratory in light of relatively small recruitment numbers, when age, BMI, and insulin resistance are accounted for, the PFAAs do not appear to be related to 25(OH)D3 or the active 1,25(OH)2D3 in this Caucasian population, nor do they appear to be associated with vitamin D deficiency, suggesting that future studies must account for these factors in the analysis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association of Vitamin D with Perfluorinated Alkyl Acids in Women with and without Non-Obese Polycystic Ovary Syndrome

Butler,

Sathyapalan,

Das

et al. 2024

Biomedicines

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Microbially Produced Bile Acids are Associated with High Levels of IgG Autoantibodies and Worse Mental Well-being in Fibromyalgia Subjects

Jakobsson,

Carlsson,

Erngren

et al. 2024

Preprint

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Fibromyalgia (FM) is a disease primarily associated with chronic widespread pain, but other common symptoms are anxiety and depression. We previously proposed that autoimmunity contributes to FM based on findings of increased immunoglobulin G binding to satellite glial cells (anti-SGC IgG) in FM subjects compared to healthy controls (HC). Emerging research suggests that an altered gut microbiota composition is connected to psychological symptoms in FM rather than pain. Gut microbiota can produce or alter bile acids (BAs) and short-chain fatty acids (SCFAs), which have immune and inflammatory functions. Here, we investigate alterations in BA and SCFA concentrations in FM subjects compared to healthy controls (HC) and potential associations with FM symptoms and anti-SGC IgG levels. Bile acids and SCFAs were quantified using liquid chromatography coupled with high-resolution mass spectrometry and anti-SGC IgG levels were assessed with immunocytochemistry. The correlations between FM symptoms, anti-SGC IgG levels, and serum concentrations of 24 BAs and 11 SCFAs in 35 FM subjects and 32 matched HC were examined. Fibromyalgia subjects had significantly higher levels of microbially produced BAs than HC. Strikingly, 11 out of 24 BAs were significantly elevated in FM subjects with high, compared to those with low, anti-SGC IgG levels. Concentrations of specific BAs were associated with increased disease severity and worse mental well-being. These results revealed increased levels of secondary BAs in FM subjects compared to HC. The strong association between BAs, anti-SGC IgG levels, and mental well-being may help elucidate the importance of BAs in the psychological symptoms of FM.

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Combining the targeted and untargeted screening of environmental contaminants reveals associations between PFAS exposure and vitamin D metabolism in human plasma

Abstract: We have developed, validated, and applied a method for targeted and untargeted screening of environmental contaminants in human plasma using liquid chromatography high-resolution mass spectrometry (LC-HRMS). The method was optimized...

Cited by 2 publications

References 67 publications

Association of Vitamin D with Perfluorinated Alkyl Acids in Women with and without Non-Obese Polycystic Ovary Syndrome

Association of Vitamin D with Perfluorinated Alkyl Acids in Women with and without Non-Obese Polycystic Ovary Syndrome

Microbially Produced Bile Acids are Associated with High Levels of IgG Autoantibodies and Worse Mental Well-being in Fibromyalgia Subjects

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