2021
DOI: 10.1016/j.ab.2021.114139
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Combining two optimized and affordable methods to assign chemoreceptors to a specific signal

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Cited by 15 publications
(22 citation statements)
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“…We have previously shown that the chemoreceptor SO_1056, which belongs to the 40H family, is involved in the attraction of S. oneidensis towards chromate [8]. The LBD of SO_1056 contains a double Cache domain (dCache_1).…”
Section: Resultsmentioning
confidence: 99%
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“…We have previously shown that the chemoreceptor SO_1056, which belongs to the 40H family, is involved in the attraction of S. oneidensis towards chromate [8]. The LBD of SO_1056 contains a double Cache domain (dCache_1).…”
Section: Resultsmentioning
confidence: 99%
“…The recombinant MBP-tagged protein (MBP-LBD1056) was produced from E. coli C600 strain containing the pMAL-LBD-1056 plasmid as previously described [8]. Briefly, the strain was grown aerobically in LB medium at 37 °C until an OD 600 of 0.5 was reached.…”
Section: Expression and Purification Of Mbp-lbd1056 Chimeric Proteinmentioning
confidence: 99%
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“…Thermo shift assay was carried out as described previously [ 44 , 45 ]. In brief, each purified protein was mixed with or without 500 μM formic acid in the presence of SYPRO Orange (Thermo Fisher Scientific, Waltham, MA, USA), which was buffered by 50 mM Tris-HCl (pH 7.4).…”
Section: Methodsmentioning
confidence: 99%