Combustion and emissions characterization of terpenes with a view to their biological production in cyanobacteria

Hellier, Paul; Al‐Haj, Lamya; Talibi, Midhat; Purton, Saul; Ladommatos, Nicos

doi:10.1016/j.fuel.2013.04.042

Cited by 48 publications

(49 citation statements)

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“…Other uses for terpenoid compounds have been found in pharmaceutical, cosmetics, and polymer industries (Beglinger, 1958;Bohlmann and Keeling, 2008;Rodrigues-Corrêa et al, 2012). Monoterpenes and sesquiterpenes have also been investigated as potential sources of renewable fuel (Monteiro and Veloso, 2004;Harvey et al, 2009;Peralta-Yahya et al, 2011;Renninger et al, 2011;Meylemans et al, 2012;Rodrigues-Corrêa et al, 2012;Hellier et al, 2013;Vallinayagam et al, 2014). Efforts have been made to understand the genetic, ecological, and physicochemical processes behind the production and accumulation of terpenoids in pine and other feedstocks (Nerg et al, 1994;Manninen et al, 2002;Martin et al, 2002;Bojovic et al, 2005;Schmidt et al, 2011;Achotegui-Castells et al, 2013;Susaeta et al, 2014).…”

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Determination of Terpenoid Content in Pine by Organic Solvent Extraction and Fast-GC Analysis

et al. 2016

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Terpenoids, naturally occurring compounds derived from isoprene units present in pine oleoresin, are a valuable source of chemicals used in solvents, fragrances, flavors, and have shown potential use as a biofuel. This paper describes a method to extract and analyze the terpenoids present in loblolly pine saplings and pine lighter wood. Various extraction solvents were tested over different times and temperatures. Samples were analyzed by pyrolysis-molecular beam mass spectrometry before and after extractions to monitor the extraction efficiency. The pyrolysis studies indicated that the optimal extraction method used a 1:1 hexane/acetone solvent system at 22°C for 1 h. Extracts from the hexane/acetone experiments were analyzed using a low thermal mass modular accelerated column heater for fast-GC/FID analysis. The most abundant terpenoids from the pine samples were quantified, using standard curves, and included the monoterpenes, α-and β-pinene, camphene, and δ-carene. Sesquiterpenes analyzed included caryophyllene, humulene, and α-bisabolene. Diterpenoid resin acids were quantified in derivatized extractions, including pimaric, isopimaric, levopimaric, palustric, dehydroabietic, abietic, and neoabietic acids.Keywords: fast-gc, pyrolysis-molecular beam mass spectrometry, cell wall chemistry, renewable materials, biofuels, bioproducts, biomaterials inTrODUcTiOn Renewable chemicals, including fuels, solvents, fragrances, flavors, and pharmaceutical compounds, can be generated or extracted from renewable biomass sources. Many types of compounds can be generated from different components of the biomass, e.g., ethanol from the carbohydrate fraction or biodiesel from the lipid components in biomass. Other biomass components, including lignin and oleoresin excretions from conifers, have also been used as a source of renewable chemicals. For example, pine oleoresin is used to generate turpentine, a solvent and source of synthetic platform chemicals such as α-pinene (Palmer, 1943;Beglinger, 1958). Terpenoids, naturally occurring organic compounds derived from isoprene units, are the primary constituents of pine oleoresin (Palmer, 1943;Bohlmann and Keeling, 2008;Rodrigues-Corrêa et al., 2012). Monoterpenes (C10), sesquiterpenes (C15), and diterpenoid resin acids (C20), the main terpenoids found in pine oleoresin, are a Abbreviations: BSTFA, N,O-Bis(trimethylsilyl)trifluoroacetamide; GC/FID, gas chromatography/flame ionization detector; LTM MACH, low thermal mass modular accelerated column heater; PLW, pine lighter wood; PS, pine sapling; py-MBMS, pyrolysis-molecular beam mass spectrometry. valuable source of chemicals with many industrial applications (Beglinger, 1958;Martin et al., 2002;Monteiro and Veloso, 2004;Bohlmann and Keeling, 2008;Harvey et al., 2009; RodriguesCorrêa et al., 2012). Turpentine is composed of monoterpenes and is used in the flavor and fragrance industry (Bohlmann and Keeling, 2008;Rodrigues-Corrêa et al., 2012). Other uses for terpenoid compounds have been found in pharmaceutical, cosmetics, and po...

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Determination of Terpenoid Content in Pine by Organic Solvent Extraction and Fast-GC Analysis

et al. 2016

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“…Sesquiterpenes have been proposed as diesel fuels, and their estimated cetane value, cloud point, and energy density are also in the range of those of diesel fuels (2,7,15,75). Recently, metabolic engineering approaches have been utilized in cyanobacteria to exploit their ability to produce isoprenoids from H 2 O and CO 2 (simplest carbon source) (14,22,58,59). The advantages associated with cyanobacteria, such as fast growth compared to that of plants and yeast, high photosynthetic rate, genetic tractability, and sequenced genomes bring them to the forefront of biofuel research.…”

Section: Figmentioning

confidence: 99%

“…Their tremendous structural and functional diversity (7,19,20) provides an alluring motivation to explore biological macromolecules as next-generation biofuels for sustainable economic and industrial growth. Their low hygroscopy, high energy density, and good fluidity at low temperatures place them at the forefront of potentially good diesel and gasoline fuel alternatives (21)(22)(23). The present minireview focuses on the engineering of homologous and heterologous metabolic pathways/genes within the prokaryotic hosts for the production of isoprenoid-based advanced biofuels.…”

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Isoprenoid-Based Biofuels: Homologous Expression and Heterologous Expression in Prokaryotes

Phulara

Chaturvedi

Gupta

2016

Appl Environ Microbiol

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bEnthusiasm for mining advanced biofuels from microbial hosts has increased remarkably in recent years. Isoprenoids are one of the highly diverse groups of secondary metabolites and are foreseen as an alternative to petroleum-based fuels. Most of the prokaryotes synthesize their isoprenoid backbone via the deoxyxylulose-5-phosphate pathway from glyceraldehyde-3-phosphate and pyruvate, whereas eukaryotes synthesize isoprenoids via the mevalonate pathway from acetyl coenzyme A (acetyl-CoA). Microorganisms do not accumulate isoprenoids in large quantities naturally, which restricts their application for fuel purposes. Various metabolic engineering efforts have been utilized to overcome the limitations associated with their natural and nonnatural production. The introduction of heterologous pathways/genes and overexpression of endogenous/homologous genes have shown a remarkable increase in isoprenoid yield and substrate utilization in microbial hosts. Such modifications in the hosts' genomes have enabled researchers to develop commercially competent microbial strains for isoprenoid-based biofuel production utilizing a vast array of substrates. The present minireview briefly discusses the recent advancement in metabolic engineering efforts in prokaryotic hosts for the production of isoprenoid-based biofuels, with an emphasis on endogenous, homologous, and heterologous expression strategies.

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“…This was established by determining the concentration of linalool that reduced absorbance (of chlorophyll α) by 50% compared to the control culture. Hellier et al [32] determined that 0.0002 g/L linalool was toxic to Synechocystis sp. PCC 6803 in 24 h, by noting that this titer caused cells to bleach.…”

Section: Mutant Library Creation Via Directed Evolutionmentioning

confidence: 99%

Increasing the tolerance of filamentous cyanobacteria to next-generation biofuels via directed evolution

et al. 2016

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Combustion and emissions characterization of terpenes with a view to their biological production in cyanobacteria

Cited by 48 publications

References 48 publications

Determination of Terpenoid Content in Pine by Organic Solvent Extraction and Fast-GC Analysis

Determination of Terpenoid Content in Pine by Organic Solvent Extraction and Fast-GC Analysis

Isoprenoid-Based Biofuels: Homologous Expression and Heterologous Expression in Prokaryotes

Increasing the tolerance of filamentous cyanobacteria to next-generation biofuels via directed evolution

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