Cometabolic degradation of dichloroethenes by Comamonas testosteroni RF2

Zálešák, Michal; Růžička, Jan; Vícha, Robert; Dvořáčková, Marie

doi:10.1016/j.chemosphere.2017.07.156

Cited by 23 publications

(17 citation statements)

References 24 publications

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“…), dichloroethene (Zalesak et al . ), 3,4‐dichloroaniline (Dejonghe et al . ), and dimethyl phthalate (Li et al .…”

Section: Resultsmentioning

confidence: 99%

“…Comamonas testosteroni is a gram-negative bacterium present in various environments such as soil, water, plants and animals. Comamonas testosteroni strains have been investigated for their ability to degrade PAHs (Pannu et al 2003), propyzamide (Zhao et al 2015), polychlorinated biphenyl (Dercova et al 1993), dichloroethene (Zalesak et al 2017), 3,4-dichloroaniline (Dejonghe et al 2003), and dimethyl phthalate (Li et al 2017), and to reduce selenium oxyanion. Little is known about C. thiooxydans.…”

Section: Isolation Of Comamonas Sp 3ah48mentioning

confidence: 99%

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Comamonas sp. 3ah48 is a dibenz[ a,h ]anthracene‐degrading bacterium that is tolerant to heavy metals

Okai

Ohki

Yamamoto

et al. 2019

Lett Appl Microbiol

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Industrialization often causes polycyclic aromatic hydrocarbon (PAH) and heavy metal contamination of soil and water. In this study, we isolated a bacterium from bottom mud water around a park of Kawasaki Port, Japan, that degrades the 5‐ring PAH dibenz[a,h]anthracene (DBA). The strain, Comamonas sp. 3ah48, degraded 29% of DBA (30 μg ml−1) in 7 days, and the degradation level increased drastically, to 59%, by the addition of glutamate to the medium. The strain also degraded 40, 14, 15 and 19% of pyrene (Pyr), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF) and benzo[g,h,i]perylene (BghiP) respectively. Benzo[a]pyrene (BaP) was degraded only when glutamate was added to the medium. Strain 3ah48 retained its degradation levels in the presence of 2 mmol l−1 Co2+, Zn2+ or Cr2+, at almost the same level as that without metal, and increased the DBA degradation level to 57% in the presence of 2 mmol l−1 Cu2+, suggesting the possibility of the presence of laccase. Significance and Impact of the Study Sixteen polycyclic aromatic hydrocarbons (PAHs) are listed as priority pollutants by the United States Environmental Protection Agency (USEPA). Information about the biodegradation of one of those PAHs, dibenz[a,h]anthracene (DBA), is limited. The present study focuses on DBA degradation by Comamonas sp. 3ah48 strain isolated around Kawasaki Port, Japan. Comamonas sp. 3ah48, cultured with the addition of glutamate to the medium, was found to increase the degradation level of DBA and to degrade DBA even in the presence of high concentrations of heavy metals.

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“…), dichloroethene (Zalesak et al . ), 3,4‐dichloroaniline (Dejonghe et al . ), and dimethyl phthalate (Li et al .…”

Section: Resultsmentioning

confidence: 99%

Section: Isolation Of Comamonas Sp 3ah48mentioning

confidence: 99%

Comamonas sp. 3ah48 is a dibenz[ a,h ]anthracene‐degrading bacterium that is tolerant to heavy metals

Okai

Ohki

Yamamoto

et al. 2019

Lett Appl Microbiol

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show abstract

“…In this study, we selected C. testosteroni as an inoculant which was originally existed in the soil and inoculated in PAHs contaminated soil. Previous studies were reported that C. testosteroni could effectively degrade PAHs (Zalesak et al, 2017). We found the relative abundance of C. testosteroni was high in CT.…”

Section: Identifying Functional Microorganisms For Pahs Degradationmentioning

confidence: 46%

“…Comamonas testosteroni (C. testosteroni) is a gram-negative bacterium belonging to the class of βproteobacteria, which is widely found in soil, activated sludge and seabed sediments. C. testosteroni can degrade many sterols and PAHs, and even use these complex organic compounds as the sole carbon source (Zalesak et al, 2017). Moreover, C. testosteroni can degrade heavy metal pollutants in the environment (Zheng et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Interaction with Endogenous Microorganisms, Comamonas testosteroni Enhanced the Degradation of Polycyclic Aromatic Hydrocarbon in Soil

Sun

Cui

et al. 2021

Preprint

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This study was to explore the functional role of Comamonas testosteroni (Ct) on soil indigenous microorganisms, and analyze the effect of Ct on PAHs degradation in PAH-contaminated soil. Results showed that inoculation of Ct could degrade naphthalene (Nap), phenanthrene (Phe), and benzo [α] pyrene (BaP) significantly. The degradation rate of Nap, Phe and BaP was 81.18%, 63.38% and 37.98% on day 25, respectively, suggesting that the low molecular weight of Nap and Phe were easier to be degraded by microorganisms than BaP. Network analysis showed that inoculation of Ct significantly increased the bacteria closely related to PAHs. Structural equation models confirmed Steroidobacter as functional bacteria could affect the degradation of Nap and BaP. Inoculated Ct could effectively enhance the synergy among indigenous bacteria to degrade PAHs. This would be helpful to understand the function of inoculated strains in PAH-contaminated soil and identify functional microorganisms of PAHs remediation.

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“…Second, it does not assimilate sugars and, thus, does not compete for carbohydrates with other environmental microbes. Third, they assimilate xeno compounds like hormone pollutant steroids , and aromatic compounds such as 3, 4-dichloroaniline, dichloroethenes and vanillate . Fourth, C. testosteroni is able to aerobically reduce heavy metals .…”

mentioning

confidence: 99%

Developing a Synthetic Biology Toolkit for Comamonas testosteroni, an Emerging Cellular Chassis for Bioremediation

Tang

Liu

2018

ACS Synth. Biol.

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Synthetic biology is rapidly evolving into a new phase that emphasizes real-world applications such as environmental remediation. Recently, Comamonas testosteroni has become a promising chassis for bioremediation due to its natural pollutant-degrading capacity; however, its application is hindered by the lack of fundamental gene expression tools. Here, we present a synthetic biology toolkit that enables rapid creation of functional gene circuits in C. testosteroni. We first built a shuttle system that allows efficient circuit construction in E. coli and necessary phenotypic testing in C. testosteroni. Then, we tested a set of wildtype inducible promoters, and further used a hybrid strategy to create engineered promoters to expand expression strength and dynamics. Additionally, we tested the T7 RNA Polymerase-P promoter system and reduced its leaky expression through promoter mutation for gene expression. By coupling random library construction with FACS screening, we further developed a synthetic T7 promoter library to confer a wider range of expression strength and dynamic characteristics. This study provides a set of valuable tools to engineer gene circuits in C. testosteroni, facilitating the establishment of the organism as a useful microbial chassis for bioremediation purposes.

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Cometabolic degradation of dichloroethenes by Comamonas testosteroni RF2

Cited by 23 publications

References 24 publications

Comamonas sp. 3ah48 is a dibenz[ a,h ]anthracene‐degrading bacterium that is tolerant to heavy metals

Comamonas sp. 3ah48 is a dibenz[ a,h ]anthracene‐degrading bacterium that is tolerant to heavy metals

Interaction with Endogenous Microorganisms, Comamonas testosteroni Enhanced the Degradation of Polycyclic Aromatic Hydrocarbon in Soil

Developing a Synthetic Biology Toolkit for Comamonas testosteroni, an Emerging Cellular Chassis for Bioremediation

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