Coming of Age: Cryo-Electron Tomography as a Versatile Tool to Generate High-Resolution Structures at Cellular/Biological Interfaces

Wang, Zuoneng; Zhang, Qingyang; Mim, Carsten

doi:10.3390/ijms22126177

Cited by 8 publications

(6 citation statements)

References 101 publications

(134 reference statements)

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“…The crosstalk between different types of transporters unfortunately remains understudied. With the advancement in membrane protein reconstitution [ 166 , 167 ], protein structural analysis of cellular events at near atomic resolution by cryo-electron tomography [ 168 ], and biophysical methodology of multi-subunit macromolecules, the existing data of cell biology, biochemistry, and structural biology on P4-ATPases and ABC lipid transporters should promote interdisciplinary biochemical, biophysical and computational approaches to elucidate the cross-talk among different lipid transporters in regulating membranes and membrane-mediated cellular signaling.…”

Section: Discussionmentioning

confidence: 99%

Lipid Transporters Beam Signals from Cell Membranes

et al. 2021

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Lipid composition in cellular membranes plays an important role in maintaining the structural integrity of cells and in regulating cellular signaling that controls functions of both membrane-anchored and cytoplasmic proteins. ATP-dependent ABC and P4-ATPase lipid transporters, two integral membrane proteins, are known to contribute to lipid translocation across the lipid bilayers on the cellular membranes. In this review, we will highlight current knowledge about the role of cholesterol and phospholipids of cellular membranes in regulating cell signaling and how lipid transporters participate this process.

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Section: Discussionmentioning

confidence: 99%

Lipid Transporters Beam Signals from Cell Membranes

et al. 2021

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“…Electroporation uses short and high-voltage electrical pulses to temporarily create small holes in membranes, increasing their permeability and allowing hydrophilic cargoes to diffuse into EVs [67]. This technique is one of the most commonly used for EV loading.…”

Section: Physical Methodsmentioning

confidence: 99%

BP-EVs: A Novel Source of EVs in the Nanocarrier Field

Lorca,

Fernández-Rhodes,

Antonio Sánchez Milán

et al. 2024

Extracellular Vesicles - Applications and Therapeutic Potential

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Extracellular vesicles (EVs) represent a complex mechanism of molecular exchange that has garnered significant attention in recent times. Nonetheless, identifying sustainable sources of biologically safe EVs remains challenging. This chapter delves into the utilization of fermented food industry by-products as a circular and secure reservoir of biocompatible EVs, dubbed as BP-EVs. BP-EVs demonstrate excellent oral bioavailability and biodistribution, with negligible cytotoxicity, and a preferential targeting capacity toward the central nervous system, liver, and skeletal tissues. The ease of editing BP-EVs is also depicted using the most common EV editing methods in this chapter. Globally, these groundbreaking findings are poised to unlock significant avenues for leveraging BP-EVs as an optimal source of biocompatible nanovesicles across a wide array of applications within the bioeconomy and biomedical fields. These applications primarily target molecule delivery into the central nervous system and skeletal tissue but are not limited to these two organism systems.

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“…It is particularly well suited to investigate protein‐membrane systems. [66] CryoET has recently been used to image the impact of Aβ on lipid vesicles. [23] Different Aβ assembly forms have been incubated with large unilamellar lipid vesicles (LUVs) containing; PC, Cholesterol and GM1 (68 : 30 : 2 by weight).…”

Section: Visualising Aβ—membrane Interactionsmentioning

confidence: 99%

Imaging Amyloid‐β Membrane Interactions: Ion‐Channel Pores and Lipid‐Bilayer Permeability in Alzheimer's Disease

Viles

2023

Angew Chem Int Ed

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The accumulation of the amyloid‐β peptides (Aβ) is central to the development of Alzheimer's disease. The mechanism by which Aβ triggers a cascade of events that leads to dementia is a topic of intense investigation. Aβ self‐associates into a series of complex assemblies with different structural and biophysical properties. It is the interaction of these oligomeric, protofibril and fibrillar assemblies with lipid membranes, or with membrane receptors, that results in membrane permeability and loss of cellular homeostasis, a key event in Alzheimer's disease pathology. Aβ can have an array of impacts on lipid membranes, reports have included: a carpeting effect; a detergent effect; and Aβ ion‐channel pore formation. Recent advances imaging these interactions are providing a clearer picture of Aβ induced membrane disruption. Understanding the relationship between different Aβ structures and membrane permeability will inform therapeutics targeting Aβ cytotoxicity.

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Coming of Age: Cryo-Electron Tomography as a Versatile Tool to Generate High-Resolution Structures at Cellular/Biological Interfaces

Cited by 8 publications

References 101 publications

Lipid Transporters Beam Signals from Cell Membranes

Lipid Transporters Beam Signals from Cell Membranes

BP-EVs: A Novel Source of EVs in the Nanocarrier Field

Imaging Amyloid‐β Membrane Interactions: Ion‐Channel Pores and Lipid‐Bilayer Permeability in Alzheimer's Disease

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