2016
DOI: 10.1038/nrg.2016.49
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Coming of age: ten years of next-generation sequencing technologies

Abstract: Since the completion of the human genome project in 2003, extraordinary progress has been made in genome sequencing technologies, which has led to a decreased cost per megabase and an increase in the number and diversity of sequenced genomes. An astonishing complexity of genome architecture has been revealed, bringing these sequencing technologies to even greater advancements. Some approaches maximize the number of bases sequenced in the least amount of time, generating a wealth of data that can be used to und… Show more

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Cited by 3,492 publications
(2,654 citation statements)
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References 130 publications
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“…In addition, it is possible with this information to estimate effective population size ( N e ; e.g., Li & Durbin, 2011) or effective number of breeders ( N b ) using LD‐based methods, as comparisons can be restricted to pairs of loci on different scaffolds, which should reduce or eliminate LD due to physical linkage. Depending on the genome size and complexity, an investment of $10k to $20k could achieve a useful reference genome with an N50 of ~100 kbp, which can be sufficient to improve data analysis as mentioned above (see Goodwin, McPherson, & McCombie, 2016) for costs per Gb for various sequencing platforms). Furthermore, the reference assembly could likely be provided by a commercial company (e.g., DoveTail, https://dovetailgenomics.com/) for this price, as long as the genome is not too large (≫3 GB) or complex (e.g., duplicated, numerous repeats), and if the initial DNA is of high molecular weight (many fragments >15–20 kb).…”
Section: Genotyping Error and Improving Data Qualitymentioning
confidence: 99%
See 1 more Smart Citation
“…In addition, it is possible with this information to estimate effective population size ( N e ; e.g., Li & Durbin, 2011) or effective number of breeders ( N b ) using LD‐based methods, as comparisons can be restricted to pairs of loci on different scaffolds, which should reduce or eliminate LD due to physical linkage. Depending on the genome size and complexity, an investment of $10k to $20k could achieve a useful reference genome with an N50 of ~100 kbp, which can be sufficient to improve data analysis as mentioned above (see Goodwin, McPherson, & McCombie, 2016) for costs per Gb for various sequencing platforms). Furthermore, the reference assembly could likely be provided by a commercial company (e.g., DoveTail, https://dovetailgenomics.com/) for this price, as long as the genome is not too large (≫3 GB) or complex (e.g., duplicated, numerous repeats), and if the initial DNA is of high molecular weight (many fragments >15–20 kb).…”
Section: Genotyping Error and Improving Data Qualitymentioning
confidence: 99%
“…There are a growing number of approaches for genome assembly using “single molecule real‐time” sequencing (SMRT‐seq) or “synthetic long‐read” sequencing (SLR‐seq) technology (Fuentes‐Pardo & Ruzzante, 2017; Goodwin et al., 2016). The SMRT‐seq technology offered by PacBio (http://www.pacb.com/) produces read lengths of ~10 kbp (some >60 kbp).…”
Section: Genotyping Error and Improving Data Qualitymentioning
confidence: 99%
“…Another principle underlies the method of calculating gene expression in the NGS technology (see [5,4]). In this technology the DNA-polymerase attaches only one fluorescently modified nucleotide being complementary to the base template.…”
Section: Resultsmentioning
confidence: 99%
“…There exist two main technology of genes expression determination today: DNA-microarrays [2,3] and Next Generation Sequence (NGS) technology [4,5,6,7,8]. Historically the first technology was the technology of the DNA-microarray.…”
Section: Introductionmentioning
confidence: 99%
“…These include the lower base level accuracy, as well as the current difficulty in sequencing more than 12 isolates in parallel (as compared to Illumina) 28,32 .…”
Section: Nanopore: a New And Emerging Technology For Real-time Analysismentioning
confidence: 99%