Commensal Bacillus siamensis LF4 ameliorates β-conglycinin induced inflammation in intestinal epithelial cells of Lateolabrax maculatus

Liu, Zi-Yan; Yang, Hongling; Ding, Xi-Yue; Li, Sha; Cai, Guo-He; Ye, Jidan; Zhang, Chunxiao; Sun, Yun‐Zhang

doi:10.1016/j.fsi.2023.108797

Cited by 7 publications

(1 citation statement)

References 32 publications

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“…The excessive aggregation of misfolded or unfolded proteins in the ER lumen can activate a UPR, in which ERS protects cells against external damage . However, persistent, excessive ERS leads to cell apoptosis, which is an important indicator of the pathogenesis and aggravation of inflammatory bowel disease.…”

Section: Discussionmentioning

confidence: 99%

4-Phenylbutyric Acid Attenuates Soybean Glycinin/β-Conglycinin-Induced IPEC-J2 Cells Apoptosis by Regulating the Mitochondria-Associated Endoplasmic Reticulum Membrane and NLRP-3

Wang,

Zhang,

Jiang

et al. 2024

J. Agric. Food Chem.

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Glycinin (11S) and β-conglycinin (7S) from soybean (glycine max) cause diarrhea and intestinal barrier damage in young animals. Understanding the mechanisms underlying the damage caused by 7S and 11S, it is vital to develop strategies to eliminate allergenicity. Consequently, we investigated 7S/11S-mediated apoptosis in porcine intestinal epithelial (IPEC-J2) cells. IPEC-J2 cells suffered endoplasmic reticulum stress (ERS) in response to 7S and 11S, activating protein kinase RNA-like ER kinase, activating transcription factor 6, C/EBP homologous protein, and inositol-requiring enzyme 1 alpha. 4-Phenylbutyric acid (4-PBA) treatment alleviated ERS; reduced the NLR family pyrin domain containing 3, interleukin-1β, and interleukin-18 levels; inhibited apoptosis; increased mitofusin 2 expression; and mitigated Ca 2+ overload and mitochondria-associated ER membrane (MAM) dysfunction, thereby ameliorating IPEC-J2 injury. We demonstrated the pivotal role of ERS in MAM dysfunction and 7S-and 11Smediated apoptosis, providing insights into 7S-and 11S-mediated intestinal barrier injury prevention and treatment.

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Section: Discussionmentioning

confidence: 99%