Comment on: Technical Issues Behind Molecular Monitoring in Chronic Myeloid Leukemia

Authors’ Reply to Crampe and Langabeer: Technical Issues Behind Molecular Monitoring in Chronic Myeloid Leukemia

“…This limitation could be overcome by next-generation sequencing [5], but the technology required is far from being broadly accessible for routine applications, and the genomic approach is limited to pilot studies. As pointed out [1], another technical opportunity for the analysis of both DNA and RNA, is the use of digital PCR. Crampe and Langabeer also dealt with parameters such as LOD, LOQ and UOM, which are crucial for the correct characterization of analytical methods as demanded by the continuous evolution of the laboratory equipments.…”