Commercial and Homemade Extremely Dilute Hypochlorous Acid Solutions Are Bactericidal Against Staphylococcus aureus and Escherichia coli In Vitro

Ramey, David W.; Kinde, Hailu

doi:10.1016/j.jevs.2014.12.004

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…HOCl eradicates bacteria by inhibiting bacterial growth, cell division, and protein synthesis; oxidizing sulfhydryl enzymes and amino acids; decreasing adenosine triphosphate production; breaking DNA; and depressing DNA synthesis 22,31,32 . HOCl is already used as a wound cleansing agent 33 , and published work demonstrates that HOCl acts as an antimicrobial, anti-biofilm agent that also promotes wound healing 30,34–37 . Sakarya et al .…”

Section: Introductionmentioning

confidence: 99%

Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms

Kiamco

Zmuda

Mohamed

et al. 2019

Sci Rep

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Biofilm formation causes prolonged wound infections due to the dense biofilm structure, differential gene regulation to combat stress, and production of extracellular polymeric substances. Acinetobacter baumannii , Staphylococcus aureus , and Pseudomonas aeruginosa are three difficult-to-treat biofilm-forming bacteria frequently found in wound infections. This work describes a novel wound dressing in the form of an electrochemical scaffold (e-scaffold) that generates controlled, low concentrations of hypochlorous acid (HOCl) suitable for killing biofilm communities without substantially damaging host tissue. Production of HOCl near the e-scaffold surface was verified by measuring its concentration using needle-type microelectrodes. E-scaffolds producing 17, 10 and 7 mM HOCl completely eradicated S. aureus , A. baumannii , and P. aeruginosa biofilms after 3 hours, 2 hours, and 1 hour, respectively. Cytotoxicity and histopathological assessment showed no discernible harm to host tissues when e-scaffolds were applied to explant biofilms. The described strategy may provide a novel antibiotic-free strategy for treating persistent biofilm-associated infections, such as wound infections.

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Section: Introductionmentioning

confidence: 99%

Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms

Kiamco

Zmuda

Mohamed

et al. 2019

Sci Rep

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“…HOCl is used as a wound cleansing agent (10). Studies show that HOCl acts as an antimicrobial and anti-biofilm agent that also supports wound healing (11,(12)(13)(14)(15). VAC technology is a mechanism that generates a negative pressure and acts by reducing exudate, inflammation and edema, and accelerates the healing process.…”

Section: Discussionmentioning

confidence: 99%

“…HOCl is used as a wound cleansing agent (10). Studies show that HOCl acts as an antimicrobial and anti-biofilm agent that also supports wound healing (11,(12)(13)(14)(15). Sakarya et al showed that a stable HOCl solution reduces the number of cells in biofilms and has positive effects on in-vitro fibroblast and keratinocyte migration (12).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Conventional Wet Dressing and Vacuum Assisted Closure with Hypochlorous Acid Application in Fournier Gangrene Treatment

Kurt

DEMİRCİ²,

ÖZKAYA

2022

Harran Üniversitesi Tıp Fakültesi Dergisi

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Amaç: Bakteriyel toksinler, lokal doku hasarına ve nekroza neden olur. Önemli miktarda doku nekrotik hale gelene kadar bu devam edebilir. Bakteriyel toksinlerin nötralizasyonu sonuçları iyileştirmektedir. Hipoklorik asit’in düşük konsantrasyonları, yaradaki ve çevre dokulardaki normal hücrelerde sitotoksisiteye neden olmadan antimikrobiyal etkili olabilmektedir. Fournier Gangreni gelişen hastalarda, yapılan operasyon sonrası uygulanan Vakum Destekli Kapatma eşliğinde yaraya hipoklorik asit (% 0.01 w/v) uygulaması ile geleneksel ıslak pansuman tedavisi uygulanan hastaların sonuçlarını karşılaştırmayı amaçlayan bir çalışma hazırladık. Materyal ve Metod: Merkezimizde 2009-2019 yılları arasında Fournier Gangreni nedeniyle debridman uygulanan 73 hasta retrospektif olarak değerlendirdi. İki grup halinde ele alındılar; debridmandan sonra Vakum Destekli Kapatma ile Hipoklorik asit uygulananlar (A Grubu, n =30) ve geleneksel ıslak pansuman uygulananlar (Grup B, n = 42). Bakterileri ve bakteriyel toksinleri yok ettiği bilinen Hipoklorik asit (% 0.01 w/v), pH’ı 4-5 olan salin içinde Fournier Gangreni’li 30 hastaya günde 5-6 kez uygulandı. Vakum Destekli Kapatma kullanılarak 5-10 ml % 0.01 Hipoklorik asit yaraya uygulanıp aspire edildi. Islak pansuman uygulamasında ise günde 3 kez, izotonik ile ıslatılan spançlarla yara temizlendikten sonra yara yüzeyine ve derinliğine uygun sayıdaki ıslak spançlara mupirosin kalsiyum ve rifamisin uygulanarak yara kapatıldı. Toplanan veriler karşılaştırıldı. Anlamlılık için 0.05’ten küçük p değeri kabul edildi. Bulgular: Vakum Destekli Kapatma ile Hipoklorik asit uygulanan 30 hastanın hastanede yatış süresi ve yara iyileşme süresi daha kısaydı (p= 0.018) ve ikincil debridman gereken hasta sayısı da daha düşüktü (p = 0.026). Sonuç: Vakum Destekli Kapatma ile Hipoklorik asit uygulaması, daha kısa hastanede yatış süresi ve daha hızlı iyileşme süresi sağlayan etkili, ameliyat sonrası için başarılı bir yara tedavi yöntemidir. Bakteri kaynaklı ve zarar gören hücrelerden salınan toksinler nedeniyle ortaya çıkan toksisite ve immun işlevsizlik Vakum Destekli Kapatma eşliğinde 0.01 Hipoklorik asit uygulaması ile hafifletilebilir. Uygun Fournier Gangreni vakalarında kolay uygulanabilir ve ucuz olan bu yöntemi öneriyoruz.

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“…However, because it is a gas, chlorine has little practical use. Instead, many compounds that release chlorine find wide application, including hypochlorite and organic and inorganic chloramines [ 4 ]. Hypochlorous acid (also referred to as electrolyzed water) is an oxyacid of chlorine (with formula HClO) containing monovalent chlorine that acts as an oxidizing or reducing agent [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity effects of electrolytically generated hypochlorous acid-treated pathogenic microorganisms by isothermal kinetic simulation

Tsai

Liu

Lin

et al. 2022

J Therm Anal Calorim

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This study involves isothermal kinetic simulation to evaluate the parameters of inhibition conditions for Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) of high-risk pathogens. This is because the new type of the 2019 novel coronavirus (2019-nCoV) is continuously spreading and the importance of public health issues. Environmental disinfection and personal wearing of masks have become important epidemic prevention measures. Selection of concentration kinetics could be estimated best for E. coli and S. aureus of pathogens, 2.74 × 10 4 and 10 5 and 2.44 × 10 4 and 10 5 colony-forming units (CFU mL –1 ), by isothermal micro-calorimeter (TAM Air) tests, respectively. Comparisons were made of different doses of 0–70 ppm (in 20 mL test ampoule) hypochlorous acid treatment for conducting n th-order and autocatalytic reaction simulation to evaluate the inhibition reaction parameters, which determined the autocatalytic kinetic model that was beneficially applied on the E. coli and S. aureus . We developed the inhibition reaction parameters of the pathogens, which included the activation energy ( E a ), the natural logarithm of pre-exponential factor (ln k 0 ), the enthalpy of inhibition microbial growth reaction ( ∆H ), inhibition microbial growth, and the inhibition growth analysis. Overall, we conducted isothermal kinetic simulation to understand the antimicrobial activity effects of electrolytically generated hypochlorous acid-treated pathogenic microorganisms, which will provide reference for public health and medical-related fields for SDG3, and can contribute to ensuring human health and hygiene.

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Commercial and Homemade Extremely Dilute Hypochlorous Acid Solutions Are Bactericidal Against Staphylococcus aureus and Escherichia coli In Vitro

Cited by 5 publications

References 14 publications

Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms

Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms

Comparison of Conventional Wet Dressing and Vacuum Assisted Closure with Hypochlorous Acid Application in Fournier Gangrene Treatment

Antimicrobial activity effects of electrolytically generated hypochlorous acid-treated pathogenic microorganisms by isothermal kinetic simulation

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