Commercial aspects of oocyte retrieval and in vitro fertilization (IVF) for embryo production in problem cows

Looney, C.R.; Lindsey, B.R.; Gonseth, C.L.; Johnson, Dan L.

doi:10.1016/s0093-691x(05)80050-0

Cited by 188 publications

(89 citation statements)

References 18 publications

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“…From 124 follicles punctured, oocyte recovery rate of 48% was recorded. In another study [127], an oocyte recovery rate of 50-70% has been reported in cows.…”

Section: Ultrasonographic Folliculocentesismentioning

confidence: 86%

Bovine Reproductive Ultrasonography: A Review

Ribadu

Nakao

1999

Journal of Reproduction and Development

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Abstract. The advent of ultrasonography over a decade ago has contributed tremendously in the field of bovine reproduction. Ultrasound imaging has shown that cattle exhibit 2 or 3 waves of follicular development during an estrous cycle. Ultrasonography allows monitoring of individual follicles as they grow and/or regress over time and patterns of follicular development can thus be determined with relative precision. Other important applications of ultrasonography in bovine reproduction includes; pregnancy diagnosis, fetal sex determination, folliculocenteses, diagnosis of abnormalities of the reproductive organs, monitoring of treatment of ovarian cysts, monitoring of postpartum genital resumption, ultrasound-guided centesis and male genital ultrasonography. Recent applications of ultrasonography in bovine reproduction includes doppler ultrasonography for genital blood flow and mammary ultrasonography. The use of ultrasonography is indispensable in most studies in which morphology (structure) and function are correlated. In this paper, the literature pertaining to the use of ultrasonography in bovine reproduction is reviewed. The applications and limitations of ultrasonography are highlighted. The non-invasive nature of ultrasonography makes it an excellent clinical and research tool in bovine reproduction.

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“…From 124 follicles punctured, oocyte recovery rate of 48% was recorded. In another study [127], an oocyte recovery rate of 50-70% has been reported in cows.…”

Section: Ultrasonographic Folliculocentesismentioning

confidence: 86%

Bovine Reproductive Ultrasonography: A Review

Ribadu

Nakao

1999

Journal of Reproduction and Development

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“…COCs were collected in individual 50 ml TCM-air drops, at pH 7.2 (TCM199; Sigma-Aldrich), containing 50 mg/ml gentamicin sulfate (Sigma-Aldrich), 0.2 mM Na pyruvate (Sigma-Aldrich), 4.2 mM NaHCO 3 (Roth), and 1 mg/ml BSA (FAF; Sigma-Aldrich) for each animal. Subsequently, the quality of the COCs was evaluated based on the homogeneity of the cytoplasm and the surrounding cumulus cells and only COCs morphologically classified into classes I and II (Looney et al 1994, Goodhand et al 1999 were used in this study.…”

Section: Ultrasound Equipment and Oocyte Retrievalmentioning

confidence: 99%

DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors

Diederich¹,

Hansmann²,

Heinzmann³

et al. 2012

Reproduction

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The developmental capacity of oocytes from prepubertal cattle is reduced compared with their adult counterparts, and epigenetic mechanisms are thought to be involved herein. Here, we analyzed DNA methylation in three developmentally important, nonimprinted genes (SLC2A1, PRDX1, ZAR1) and two satellite sequences, i.e. 'bovine testis satellite I' (BTS) and 'Bos taurus alpha satellite I' (BTaS). In parallel, mRNA expression of the genes was determined by quantitative real-time PCR. Oocytes were retrieved from prepubertal calves and adult cows twice per week over a 3-week period by ultrasound-guided follicular aspiration after treatment with FSH and/or IGF1. Both immature and in vitro matured prepubertal and adult oocytes showed a distinct hypomethylation profile of the three genes without differences between the two types of donors. The methylation status of the BTS sequence changed according to the age and treatment while the methylation status of BTaS sequence remained largely unchanged across the different age and treatment groups. Relative transcript abundance of the selected genes was significantly different in immature and in vitro matured oocytes; only minor changes related to origin and treatment were observed. In conclusion, methylation levels of the investigated satellite sequences were high (O50%) in all groups and showed significant variation depending on the age, treatment, or in vitro maturation. To what extent this is involved in the acquisition of developmental competence of bovine oocytes needs further study.

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“…In the cattle embryo transfer industry, embryos increasingly are produced totally in vitro from immature oocytes that are collected by repeated ovum pick-up or from slaughterhouse ovaries; then matured, fertilized and cultured in vitro up to the blastocyst stage (for review see: 14,18). Efficient utilization of in vitro-produced embryos requires that they are able to be frozen before their transfer later in different locations.…”

Section: ~Troductionmentioning

confidence: 99%

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Díez

Heyman

Bourhis³

et al. 2001

Theriogenology

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To study the effect of partial removal of intracytoplasmatic lipids from bovine zygotes on their in vitro and in vivo survival, presumptive zygotes were delipidated by micromanipulation and cocultured with Veto cells in B2+10% FCS. Blastocyst rates of delipidated (n=960), sham (centrifuged but not delipidated, n=830) and control embryos (n=950) were 42.1, 42.3 and 39.9% respectively (P > 0.05). Day 7 blastocysts derived from delipidated zygotes had a mean of 123.9 + 45.6 nuclei compared to 137.5 + 32.9 for control blastocysts (P > 0.05). The full-term development of delipidated blastocysts after single transfer to recipients was similar to that of control IVF blastocysts (41.2% vs 45.4% respectively). To assess the effect of delipidation on the embryo tolerance to freczing/thawing, delipidated (n=73), control (n=67) and shmn (n=50) Day 7 blastocysts were frozen in 1.36 M glycerol + 0.25 M sucrose in PBS. After thawing, embryos were cocultured for 72 h with Vero cells in B2+10% FCS. Survival rates at 24 h were not significantly different between groups. However, in the delipidated group, the survival rate after 48 h in culture was significantly higher than in the control group (56.2 vs 39.8, P < 0.02), resulting in a higher hatching rate after 3 days in culture (45.2 vs 22.4, P < 0.02). Pregnancy rates for delipidated and control frozen/thawed embryos were respectively 10.5 and 22.2% (P > 0.05). Electron microscopic observations showed much fewer lipid droplets (and smaller) in delipated blastocysts than in controls. Taken together, our data show that delipidation of one cell stage bovine embryos is compatible with their normal development to term and has a beneficial effect on their tolerance to freezing and thawing at the blastocyst stage. This procedure, however, alters the developmental potential of such blastocysts, suggesting that maternally inherited lipid stores interfere with metabolic recovery after thawing.

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Commercial aspects of oocyte retrieval and in vitro fertilization (IVF) for embryo production in problem cows

Cited by 188 publications

References 18 publications

Bovine Reproductive Ultrasonography: A Review

Bovine Reproductive Ultrasonography: A Review

DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

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