Commercial fertilizers and organic additives in orchid micropropagation

Abstract

In vitro cultivation strategies promise conservation and micropropagation of vulnerable or economically exciting plants, especially in Orchidaceae. Numerous reports are available for an extensive contingent of orchids of the most varied genera, and the main issue is related to the behavior of species during the process of germination and growth concerning the nutrients used in the culture media. In Orchidaceae, several evidence support that each species responds differently and specifically according to the biotic and abiotic conditions available for in vitro growth. The main concern is to seek simplified culture media that promote germination and the development of healthy plants on a large scale. This study assessed the behavior of Catasetum fimbriatum and Catasetum macrocarpum in culture media containing commercial fertilizers supplemented with organic additives, compared with traditional media, an economically more viable strategy for micropropagation. Seeds were cultured on traditional nutrient media or with commercial fertilizer and organic additives in different combination. The analysis of plant growth after 120 days in vitro showed that the best treatment in both species was the medium containing commercial foliar fertilizer Peters NPK ® 10:30:20 supplemented with potato and banana pulp.

Effect of Culture Medium, Growth Regulators and Organic Additives on Shoot Regeneration from Hybrid-Seeds of Phalaenopsis

“…Shoot initiation and proliferation are accelerated when organic additives are added to culture media, either singly or in combination. For in vitro cultures to succeed, different kinds and amounts of organic nutrients must be used, depending on the species and genotype [28]. A number of research were done to determine how organic additions affected shoot growth, regeneration, and rhizogenesis, which in turn produced healthy plantlets.…”

“…A number of research were done to determine how organic additions affected shoot growth, regeneration, and rhizogenesis, which in turn produced healthy plantlets. [27,17,28].…”

Plant growth regulators and organic additives on the proliferation of protocorm-like bodies and plantlet regeneration of Cattleya gaskelliana (N.E.Br.) B.S.Williams

“…After in-depth investigations, researchers have identified the optimal medium for in vitro regeneration, multiplication, and growth of orchid seedlings, using a combination of organic additives and growth regulators. Organic additives contain carbohydrates, vitamins, and amino acids, which enhance the effectiveness of the propagation of orchid plants and decrease laboratory production costs (Moraes et al, 2020;Poniewozik, Szot, & Parzymies, 2021;Samiei et al, 2021). However, few studies have investigated the clonal micropropagation of C. gaskelliana (Mercado & Jaimes, 2022).…”