Commercial gold nanocolloid inhibits synthesis of IL-2 and proliferation of porcine T lymphocytes

Małaczewska, Joanna; Wójcik, Roman; Kaczorek, Edyta; Rękawek, Wojciech; Siwicki, Andrzej K.

doi:10.1016/j.rvsc.2016.10.013

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…However, the majority of these studies have been carried out in solution. [20][21][22][23][24] A more biologically relevant model for biomimetic matrices intended for supporting and promoting cell inltration would include evaluation of the migration and polarization of macrophages, which are critical in the body's immune/inammatory response to implanted biomaterials. Such responses will ultimately dictate degradation and bioincorporation of the matrix within the host.…”

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confidence: 99%

Multi-functional thermo-crosslinkable collagen-metal nanoparticle composites for tissue regeneration: nanosilver vs. nanogold

et al. 2017

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confidence: 99%

Multi-functional thermo-crosslinkable collagen-metal nanoparticle composites for tissue regeneration: nanosilver vs. nanogold

et al. 2017

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“…Both unstimulated and mitogen-stimulated PBMCs were cultured for 72 h in the presence of the same three doses of EV-E (MOI = 10, 1 or 0.1). Afterwards, cells were harvested, washed and stained with fluorochrome-conjugated monoclonal antibodies specific to bovine CD4, CD8, WC1 or CD21 markers (all from Bio-Rad Laboratories Inc., Hercules, CA, USA), as described before ( 24 ). The properties of antibodies used in the study are summarised in Table 2 .…”

Section: Methodsmentioning

confidence: 99%

Enterovirus E infects bovine peripheral blood mononuclear cells. Implications for pathogenesis?

Małaczewska,

Wróbel,

Kaczorek-Łukowska

et al. 2023

Journal of Veterinary Research

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Introduction Enterovirus E (EV-E) is a common viral pathogen endemic in cattle worldwide. Little is known, however, about its potential interactions with bovine immune cells. Material and Methods The EV-E-permissiveness of bovine peripheral blood mononuclear cells (PBMCs) was evaluated. The infectious titres of extracellular virus were measured and the intracellular viral RNA levels were determined by reverse transcription quantitative PCR after cell inoculation. The effects of EV-E on cell viability and proliferative response were investigated with a methyl thiazolyl tetrazolium bromide reduction assay, the percentages of main lymphocyte subsets and oxidative burst activity of blood phagocytes were determined with flow cytometry, and pro-inflammatory cytokine secretion was measured with an ELISA. Results Enterovirus E productively infected bovine PBMCs. The highest infectious dose of EV-E decreased cell viability and T-cell proliferation. All of the tested doses of virus inhibited the proliferation of high responding to lipopolysaccharide B cells and stimulated the secretion of interleukin 1β, interleukin 6 and tumour necrosis factor α pro-inflammatory cytokines. Conclusion Interactions of EV-E with bovine immune cells may indicate potential evasion mechanisms of the virus. There is also a risk that an infection with this virus can predispose the organism to secondary infections, especially bacterial ones.

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“…Both unstimulated and mitogen‐stimulated PBMC were cultured in 48‐well plates for 72 hr in the presence of different concentrations of nisin. Then, the cells were stained for flow cytometry as previously described (Małaczewska, Wójcik, Kaczorek, Rękawek, & Siwicki, ). The following monoclonal antibodies (mAB, Beckton Dickinson Biosciences) were used for cell staining: PerCP‐Cy5.5‐conjugated mouse anti‐pig CD3 (IgG2a, clone: BB23‐8E6‐8C8), Alexa Fluor 647‐conjugated mouse anti‐pig CD4 (IgG2b, clone: 74‐12‐4) and PE‐conjugated mouse anti‐pig CD8α (IgG2a, clone: 76‐2‐11).…”

Section: Methodsmentioning

confidence: 99%

In vitro immunomodulatory effect of nisin on porcine leucocytes

Małaczewska

Kaczorek-Łukowska

Wójcik

et al. 2019

Animal Physiology Nutrition

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Nisin, a lantibiotic bacteriocin, has been used for years as a natural food preservative. In addition to its antimicrobial activity, nisin also shows immunomodulatory properties, and the nisin‐producing Lactococcus lactis strain has been successfully tested as a probiotic in weaned piglets. However, the impact of nisin on porcine immune cells has not yet been explored. The objective of the present study was to examine the in vitro immunomodulatory effect of nisin on porcine peripheral blood leucocytes. The whole heparinized blood samples or freshly isolated peripheral blood mononuclear cells (PBMCs) were incubated with different nisin concentrations (0, 1.56, 3.125, 6.25, 12.5, 25 or 50 µg/ml) for 1, 24, 48 or 72 hr. Escherichia coli bacteria were used to stimulate blood phagocytes, while concanavalin A and lipopolysaccharide from E. coli were used as mitogens. Control cells remained unstimulated. MTT colorimetric assay was used to evaluate PBMCs viability and mitogenic response. Phagocyte activity and T‐cell proliferation were measured by flow cytometry. Flow cytometer was also used for immunophenotyping of T cells. Cytokine levels in the culture media were determined using commercial immunoassay (ELISA) kits. The highest concentration of nisin exhibited proliferative activity (p ˂ 0.05), stimulated interleukin‐1 beta (IL‐1β) and interleukin‐6 (IL‐6) production (both at p ˂ 0.001), and increased the percentage of CD4+CD8+ T cells (p ˂ 0.001) among unstimulated leucocytes. After cell stimulation, however, the highest nisin concentration showed antiproliferative activity (p ˂ 0.05), decreased phagocytic functions (p ˂ 0.05) and inhibited the synthesis of IL‐6 (time‐ and concentration‐dependent effect). As a typical bacterial product, nisin had a stronger impact on innate immune cells, and its effect on T cells was likely a consequence of the modulation of the activity of antigen‐presenting cells. Nisin may be a good candidate as an immunomodulator in pig breeding.

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Commercial gold nanocolloid inhibits synthesis of IL-2 and proliferation of porcine T lymphocytes

Cited by 5 publications

References 26 publications

Multi-functional thermo-crosslinkable collagen-metal nanoparticle composites for tissue regeneration: nanosilver vs. nanogold

Multi-functional thermo-crosslinkable collagen-metal nanoparticle composites for tissue regeneration: nanosilver vs. nanogold

Enterovirus E infects bovine peripheral blood mononuclear cells. Implications for pathogenesis?

In vitro immunomodulatory effect of nisin on porcine leucocytes

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