Commercial products to preserve specimens for tuberculosis diagnosis: a systematic review

Reeve, Byron W P; McFall, Sally M.; Song, Rinn; Warren, Robin M.; Steingart, Karen R; Theron, Grant

doi:10.5588/ijtld.17.0816

Cited by 10 publications

(10 citation statements)

References 32 publications

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“…Since the stability and integrity of viral RNA for molecular detection is a significant concern during fieldwork, this systematic review focused on the evaluation of FTA cards® as a strategy for detection of these pathogens. However, successful applications of these cards have been also reported for DNA viruses and bacteria (Dong et al, 2017;Reeve et al, 2018). For instance, the use of FTA cards® have been proved effective as a means of sample storage and transport for genotyping isolates of Campylobacter jejuni obtained from poultry and stored for three months at room temperature (Sierra-Arguello et al, 2016).…”

Section: Limitationsmentioning

confidence: 99%

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A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Cardona-Ospina

Villalba-Miranda

Palechor-Ocampo

et al. 2019

Preventive Veterinary Medicine

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A B S T R A C TBackgroundDetection and characterization of viral RNA pathogens from fieldwork are challenging due to the instability of the RNA molecule. FTA cards® have proved useful for sample storage and latter identification of pathogens with importance for agricultural, animal and human health: however, for optimal handling, processing, and biosafety measures are not well-established.ObjectiveThis systematic review aims to summarize the reported effectiveness of FTA cards® for storage and transport of viral RNA, as well as the conditions for their handling and use in downstream processes. Finally, the biosafety measures required to protect researchers and clinical lab workers are considered.MethodsWe performed a systematic review following the PRISMA statement. We searched MEDLINE (PubMed), Scopus and Web of Science using the keywords "FTA cards" AND "RNA". Articles were screened by title and abstract, and after examination of inclusion and exclusion criteria, relevant information was extracted. The quality of the studies was assessed, and the evidence was qualitatively summarized.ResultsA total of 175 records were retrieved, and 11 additional documents were found by checking references of the eligible articles. A total of 47 articles were included. Samples from animals accounted for 38.3% of the publications, which identified viruses that cause disease in poultry, wild birds, suids, or bovids. Three different methods for RNA extraction were reported. Other factors that vary across reports include the size of RNA amplicon, storage temperature, and duration of storage. Only fourteen articles tested the inactivation of the virus on the FTA card®, and in one case, the virus remained infective.ConclusionFTA cards® could be a suitable option for RNA virus storage and transport for fieldwork in areas where proper conditions for RNA preservation are difficult to achieve. Three different protocols have been used for RNA detection from this matrix. Biospecimens in the form of dried blood spots should be considered potentially infectious unless specifically treated to inactivate viral pathogens. technology consists of filter paper impregnated with a patented chemical mixture that contains chemical denaturants and a free radical scavenger. The chemical mixture lyses cells and organelles, prevents overgrowth of bacteria, and denatures proteins. The DNA is captured in the matrix and remains tightly bound while proteins and inhibitors are washed. This keeps DNA stable during long-term storage at room temperature (GE Healthcare, 2019).

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Section: Limitationsmentioning

confidence: 99%

“…This method is attractive for several applications, including research of bacterial genetics, as DNA can be maintained at ambient temperature (Reeve et al, 2018). FTA cards® have also been used for storing samples and molecular detection of both DNA and RNA viruses.…”

mentioning

confidence: 99%

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Cardona-Ospina

Villalba-Miranda

Palechor-Ocampo

et al. 2019

Preventive Veterinary Medicine

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“…We conducted a systematic review done as part of a WHO process to directly inform policy on products for TB diagnosis. [78] We also investigated the South African (SA) TB care cascade, which is a tool by policymakers to set goals for strengthening the TB programme. [79] Furthermore, we described innovative approaches for how material typically discarded by laboratories can be used for detailed drug susceptibility testing (thus alleviating the need for additional specimen collection).…”

Section: Researchmentioning

confidence: 99%

Tuberculosis research in South Africa over the past 30 years: From bench to bedside

et al. 2019

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This open-access article is distributed under Creative Commons licence CC-BY-NC 4.0. The South African Medical Research Council Centre for Tuberculosis Research has a rich history of high-impact research that has influenced our understating of this hyper-epidemic which is further exacerbated by the emergence and spread of drug-resistant forms of the disease. This review aims to summarise the past 30 years of research conducted in the Centre which has influenced the way that tuberculosis (TB) is diagnosed and treated. The review includes the development of new technologies for rapid screening of people with probable TB and the repurposing of human diagnostics for wildlife conservation.

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“…Accurate, sensitive, and high-quality diagnostic testing, usually performed in centralized laboratories in urban centers, is crucial to improving TB diagnosis and identifying drug resistance. If specimens cannot reach centralized laboratories under conditions that preserve specimen integrity, the performance of downstream tests and their potential impact will be undermined [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis

et al. 2023

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In several low-income countries, the transport of sputa could take up to one week to reach the laboratories, resulting in increased contamination rates and a loss of growth. The aim of this study was to evaluate the effect of the OMNIgene-SPUTUM in preserving Mycobacterium tuberculosis on sputum samples simulating three hypothetical scenarios for conservation and/or decontamination: (1) sputum was mixed with OMN and conserved at room temperature for five days and then processed for culture (OMN); (2) sputum cultures followed the routine standing operating procedure at day 0 (STD); and (3) sputum samples were kept at room temperature for five days and mixed with the standard decontamination reagent (SDT5) and then processed for culture. The positivity rate based on smear microscopy was 36.4%, 29.1%, and 27.3% for STD, STD5, and OMN, respectively. The proportion of positive results by liquid culture (MGIT) was 39.1% (43/110) for STD, 26.4% (29/110) for STD5, and 20.0% for OMN (22/110). The overall concordance of liquid culture results was 51.8% (57/110): 37.3% (41/110) for negative results, 11.8% (13/110) for MTBC growth, and 2.7% (3/110) for contaminated results. The OMN arm showed better performance in solid culture than in liquid culture, with a notable reduction in contaminated results.

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Commercial products to preserve specimens for tuberculosis diagnosis: a systematic review

Abstract: Limited evidence suggests that transport products result in test performance comparable to that seen in cold chain-transported specimens.

Cited by 10 publications

References 32 publications

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Tuberculosis research in South Africa over the past 30 years: From bench to bedside

Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis

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