Commercialization of animal biotechnology

Faber, D.; Molina, Juan Antonio Maldonado; Ohlrichs, C.L.; Zwaag, D.F Vander; Ferré, L. B.

doi:10.1016/s0093-691x(02)01264-5

Cited by 69 publications

(47 citation statements)

References 82 publications

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“…Embora o estádio de blastocisto não garanta desenvolvimento posterior, é um potente indicador da competência de desenvolvimento inicial (McEvoy et al, 2000). A terapia gonadotrófica utilizando o FSH melhorou a taxa de prenhez segundo Faber et al (2003), mas no presente experimento não foi possível avaliar essa característica.…”

Section: Resultsunclassified

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Protocolos de produção in vitro de embriões na raça Gir

Ramos

Ferreira

Sá

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliaram-se os efeitos de dois protocolos de punção folicular na quantidade/qualidade dos oócitos e na produção in vitro de embriões, em vacas da raça Gir, não-lactantes. O ciclo estral foi sincronizado com cloprostenol e ao longo do experimento os animais receberam implantes auriculares de norgestomet, renovados a cada 14 dias. Os animais foram submetidos aos protocolos I (sem estimulação hormonal, com punção folicular duas vezes/semana) e II (pré-tratamento com 250 UI de FSH em doses decrescentes, três dias antes da punção folicular). Os oócitos recuperados foram levados ao laboratório em meio TALP-Hepes e submetidos à maturação. Na fecundação in vitro utilizou-se sêmen de touro Gir, previamente capacitado. Após 22 horas de fecundação, os prováveis zigotos foram co-cultivados com células da granulosa em CR2aa acrescido de 10% de soro fetal bovino. A taxa de clivagem foi avaliada 72 horas pós-fecundação e a de blastocisto 192 horas pós-fecundação. O número total de folículos foi maior (P<0,05) no protocolo II, assim como o número de folículos grandes e médios (P<0,05) e o diâmetro do maior folículo (P<0,05), sendo o número de folículos pequenos (P<0,05) menor. O total de oócitos recuperados por sessão não foi diferente entre os protocolos I e II (P>0,05). O número de oócitos de grau I e a taxa de clivagem foram maiores (P<0,05) e o número de degenerados menor protocolo II. A pré-estimulação ovariana com FSH pode melhorar a qualidade e a taxa de clivagem dos oócitos recuperados por punção folicular em animais Gir.Palavras-chave: bovino, Gir, embrião in vitro, punção folicular, FSH ABSTRACT The effect of two ovum pick-up protocols on amount/quality of oocytes retrived and on in vitro embryo production of Gyr cows was evaluated in non-lactating cycling cows with good body and reproductive conditions. Estrous cycles were synchronized with cloprostenol. During the experiment, animals received norgestomet ear implants, replaced every 14 days. Animals were submitted to protocol I (without hormonal estimulation) and protocol II (pre-stimulation with 250 IU of FSH three days before aspiration). Recovered oocytes were transported in Talp-

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Section: Resultsunclassified

“…Animais das raças zebuínas apresentam menores taxas de recuperação de oócitos e, em conseqüência, menor quantidade de embriões produzidos in vitro (Faber et al, 2003). Protocolos que utilizem superestimulação hormonal antes do início das sessões de punção folicular podem aumentar o número de oócitos colhidos in vivo.…”

Section: Introductionunclassified

Protocolos de produção in vitro de embriões na raça Gir

Ramos

Ferreira

Sá

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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“…Simple and precise methods for embryo sexing are important for management and improvement of bovine species. Polymerase chain reaction (PCR) has been used for sexing of bovine embryos [4,5] with various target sequences, including male-specific repetitive sequences [6,7] and single-copy genes on the Y chromosome, such as amelogenin [8,9] and ZFY gene [10][11][12]. When embryo sexing is performed with male-specific target sequences, one cannot distinguish the false-negative amplification caused by sampling errors or from lack of amplification in female samples.…”

mentioning

confidence: 99%

Multiplex Polymerase Chain Reaction Used for Bovine Embryo Sex Determination

Rattanasuk

Parnpai

Ketudat-Cairns

2011

Journal of Reproduction and Development

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Abstract. Widely used bovine sexing primers were compared in terms of suitability in determining the sex of bovine embryos. Under optimized multiplex PCR conditions, the ConBV/ConEY couple primers did not show accurate results when combined together in multiplex PCR, but worked well when the couple primers were used separately. The S4BF/ S4BR primers showed accurate results; however, some unexpected bands were detected. When the BY/BSP couple primers were used to determine one-cell, two-cell, four-cell and eight-cell stage embryos of known sexed SCNT-derived embryos, the results showed 100% accuracy. The BY/BSP couple primers were also able to identify the sex of one-cell and two-cell IVF-derived embryos. Key words: Bovine sex determination, Embryo sexing, Multiplex PCR (J. Reprod. Dev. 57: [539][540][541][542] 2011) ex determination of preimplantation embryos is important for manipulating the sex ratios of domestic animals at an early stage of embryo development [1,2]. A number of approaches have been used to determine the sex of bovine embryos but have failed to gain much popularity due to the lack of either accuracy or speed [3]. Simple and precise methods for embryo sexing are important for management and improvement of bovine species. Polymerase chain reaction (PCR) has been used for sexing of bovine embryos [4,5] with various target sequences, including male-specific repetitive sequences [6,7] and single-copy genes on the Y chromosome, such as amelogenin [8,9] and ZFY gene [10][11][12]. When embryo sexing is performed with male-specific target sequences, one cannot distinguish the false-negative amplification caused by sampling errors or from lack of amplification in female samples. To solve this problem, various combination of two different PCR primer sets, one for a male-specific sequence and another for a sequence common to both sexes, have been examined as a multiplex PCR to obtain internal bovine control for embryo sexing [12][13][14][15][16][17].In the present study, three widely used primer sets were compared using genomic DNA and embryo at various developmental stages. The embryos used in this study were somatic cell nuclear transfer (SCNT)-derived embryos of known sex and in vitro fertilization (IVF)-derived embryos of unknown sex. They were used as template in the multiplex PCR reactions.To compare the suitable couple primers used for bovine sex determination, 0.42 ng of high molecular weight DNA from male and female bovine fibroblast was amplified using three different sets of bovine sexing primers (Table 1) and subjected to gel electrophoresis. The PCR products were detected after ethidium bromide staining. The results in Fig. 1 suggested that the BY/BSP couple primers were able to amplify male bovine genomic DNA, which gave two fragments of 300 bp from Y-specific primers (BY) and 538 bp from bovine-specific primers (BSP) (lane 1) and a clear single band of 538 bp (lane 2) from BSP amplified female bovine genomic DNA. The S4BF/S4BR primers showed clear accurate results with bovine genomic DN...

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“…Dev. 49: [347][348][349][350][351][352][353][354][355][356][357][358][359] 2003) nimal reproductive technology has been very important in the last two decades, and the development of efficient procedures for assisted reproduction in livestock has generated multiple opportunities for commercialization [1]. The d e v e l o p m e n t o f e f f i c i e n t r e p r o d u c t i v e biotechnology has been increasingly required in pigs, since it is not only an important farm animal, b u t a l s o a m o d e l a n i m a l f o r b i o m e d i c a l applications, such as human disease processes, d r u g d e v e l o p m e n t, a n d h u m a n o r g a n xenotransplantation [2].…”

mentioning

confidence: 99%

Molecular Mechanisms Underlying Pig Oocyte Maturation and Fertilization

Sun

Nagai

2003

Journal of Reproduction and Development

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Abstract. Since the pig is not only an important farm animal, but also a model animal for biomedical applications, the development of reproductive technologies in this species has been very important. In vitro oocyte maturation and fertilization (IVM-IVF) are basic techniques for a number of oocyte-or embryo-related technologies. The practical aspects for pig oocyte IVM-IVF have been reviewed, while the molecular mechanisms underlying oocyte meiotic maturation and fertilization have not been well summarized, although accumulating data have been obtained in recent one decade. This review will focus on what is known about the molecular mechanisms of porcine oocyte maturation and fertilization such as first meiosis resumption, meiotic spindle assembly, second meiosis metaphase (MII) arrest during oocyte maturation, sperm-egg recognition and fusion, sperm acrosome reaction, second meiosis resumption, sperm chromatin decondensation, and pronucleus formation during fertilization, as well as the establishment of polyspermy block. Key words: Pig, Oocyte, Meiosis, Fertilization, Polyspermy (J. Reprod. Dev. 49: [347][348][349][350][351][352][353][354][355][356][357][358][359] 2003) nimal reproductive technology has been very important in the last two decades, and the development of efficient procedures for assisted reproduction in livestock has generated multiple opportunities for commercialization [1]. The d e v e l o p m e n t o f e f f i c i e n t r e p r o d u c t i v e biotechnology has been increasingly required in pigs, since it is not only an important farm animal, b u t a l s o a m o d e l a n i m a l f o r b i o m e d i c a l applications, such as human disease processes, d r u g d e v e l o p m e n t, a n d h u m a n o r g a n xenotransplantation [2]. Recently, somatic cell cloned pigs have been produced [3][4][5], and the productio n of gene kno ck out pig ha s been achieved by nuclear transfer of donor cells with the modification of the α (1,3)-galactosyltrnasferase (GalT) gene [6]. However, the efficiency is still extremely low. Although several systems have been established to generate embryos in vitro, the quality of embryos produced in vitro is inferior to those produced in vivo. The major problems include improper oocyte maturation, both nucleus and cytoplasmic, and polyspermy. Despite the progress, the quality of oocytes matured in vitro, defined as the potential of that oocyte to develop into a viable offspring, is still not satisfactory, limiting the improvement of other reproductive techniques in pigs [7]. Thus, the importance of the development and identification of defined in vitro conditions for oocyte maturation and fertilization s ho u ld no t be un der es t im a t ed , s inc e m os t reproductive technologies rely on these basic techniques. The technology of pig oocyte in vitro maturation, fertilization and embryo development and related problems have been well reviewed [8-

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Commercialization of animal biotechnology

Cited by 69 publications

References 82 publications

Protocolos de produção in vitro de embriões na raça Gir

Protocolos de produção in vitro de embriões na raça Gir

Multiplex Polymerase Chain Reaction Used for Bovine Embryo Sex Determination

Molecular Mechanisms Underlying Pig Oocyte Maturation and Fertilization

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