Commercially Fabricated Printed Circuit Board Sensing Electrodes for Biomarker Electrochemical Detection: The Importance of Electrode Surface Characteristics in Sensor Performance

Dutta, Gorachand; Regoutz, Anna; Moschou, Despina

doi:10.3390/proceedings2130741

Cited by 23 publications

(16 citation statements)

References 10 publications

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“…S3 in the Supplementary Information). In contrast, the detection signal for glucose was significantly higher then those from interfering species and consistent with our prior glucose detection data (Dutta et al, 2018). The present results suggest that this glucose biosensor is highly specific toward glucose and will not suffer from interference with other species or isomers that may be present in real interstitial fluid samples.…”

Section: Description Of the Detection Scheme And Glucose Measurementsupporting

confidence: 91%

Enzyme-assisted glucose quantification for a painless Lab-on-PCB patch implementation

Dutta

Regoutz

Moschou

2020

Biosensors and Bioelectronics

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In the context of an integrated Lab-on-PCB wearable patch extracting interstitial fluid from the patient via integrated microneedles, the requirements from the integrated biosensing part are quite special compared to static glucose electrochemical biosensors. Hence, in this study, a fully PCB-integrated enzymatic glucose quantification Lab-on-Chip device is presented and evaluated considering these special requirements for such a patch implementation: a) range and limit of detection compatible with interstitial fluid glucose levels of diabetic patients and b) effect of sample flow rate on the biosensing platform performance. This work employs a chronoamperometric approach for glucose detection based on covalently immobilized glucose 2 oxidase on PCB-integrated electrodes. The chronoamperometric measurements show that this platform exhibits µM range sensitivity, high specificity, and good reproducibility, and the assay can detect glucose from 10 µM to 9 mM with a lower limit of detection of 10 µM. The demonstrated detection range under continuous flow proved compatible with interstitial fluid glucose levels of diabetic patients. The sample-to-answer time of our Lab-on-PCB device is less than one minute (sample delivery of few seconds and 20 sec for electrochemical measurement), employing sample volumes of 50 μL in this instance. Increased flow rates substantially improve the platform sensitivity (1.1 μA/mM @0μL/min to 6.2 μA/mM @10μL/min), with the measured current increasing exponentially to the flow rate, as opposed to the theoretically expected much lower dependence. This work demonstrates the feasibility of Lab-on-PCB patches in terms of biosensing performance, paving the way for the first cost-effective, painless diabetes management microsystem.

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Section: Description Of the Detection Scheme And Glucose Measurementsupporting

confidence: 91%

Enzyme-assisted glucose quantification for a painless Lab-on-PCB patch implementation

Dutta

Regoutz

Moschou

2020

Biosensors and Bioelectronics

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“…The gold-plated PCB electrodes were cleaned prior to anti- Streptococcus mutans IgG immobilization by ultrasonication in acetone, ethanol, and water, respectively for 15 min followed by 30 min ultrasonication in a solution containing 5:1:1 water, ammonium hydroxide (20%), and hydrogen peroxide (30%) [24,25]. Immobilization of the anti- Streptococcus mutans IgG to the working electrode was carried out by incubating 10 µg/mL of thiolated anti- Streptococcus mutans IgG solution for 1 h at room temperature, followed by thorough rinsing with PBS, and drying with purified N 2 gas.…”

Section: Experimental Methodsmentioning

confidence: 99%

Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes

et al. 2019

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This paper reports for the first time printed-circuit-board (PCB)-based label-free electrochemical detection of bacteria. The demonstrated immunosensor was implemented on a PCB sensing platform which was designed and fabricated in a standard PCB manufacturing facility. Bacteria were directly captured on the PCB sensing surface using a specific, pre-immobilized antibody. Electrochemical impedance spectra (EIS) were recorded and used to extract the charge transfer resistance (Rct) value for the different bacteria concentrations under investigation. As a proof-of-concept, Streptococcus mutans (S. mutans) bacteria were quantified in a phosphate buffered saline (PBS) buffer, achieving a limit of detection of 103 CFU/mL. Therefore, the proposed biosensor is an attractive candidate for the development of a simple and robust point-of-care diagnostic platform for bacteria identification, exhibiting good sensitivity, high selectivity, and excellent reproducibility.

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“…During fabrication, 15 minute ultrasonication in acetone/IPA/DI water is not sufficient enough for removing organic contamination. Therefore, one more essential step of 30 minute ultrasonication in a solution containing 5:1:1 water, ammonium hydroxide (20%) and hydrogen peroxide (30%)is required [10].…”

Section: Methodsmentioning

confidence: 99%

Enhancing Potentiometric Response of Electrochemical Sensor Using Modified Ion-Sensitive Transistor

Abhinav¹,

Patkar²,

Vinchurkar³

et al. 2019

Preprint

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In this work, we present a novel combination of solid-state ion-selective electrode and field effect transistor (FET) integrated on same platform. Thus, creating an extended gate field effect transistor (EGFET). We have built an electrochemical sensing unit comprised of all solid-state electrodes, an Ag/AgCl reference electrode and ionophore coated gold electrode. Unlike any of the earlier reports, all the electrodes are integrated with a FET in a single plane of the printed circuit board (PCB), hence mitigating the issues of separate wire bonding or external connection between the sensing element and the transducer by using PCB traces. In this work, we demonstrate for the first time, the use of ion sensitive electrode (ISE) in EGFET configuration without degrading the linearity and sensitivity due to the external voltage bias. Potentiometric measurement on our K + ISE shows near Nernstian limit sensitivity (49 mV/decade), low concentration for the limit of detection (10 −6 M), and linearity over a large range of detection(10 −4 M to 1 M).

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Commercially Fabricated Printed Circuit Board Sensing Electrodes for Biomarker Electrochemical Detection: The Importance of Electrode Surface Characteristics in Sensor Performance

Cited by 23 publications

References 10 publications

Enzyme-assisted glucose quantification for a painless Lab-on-PCB patch implementation

Enzyme-assisted glucose quantification for a painless Lab-on-PCB patch implementation

Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes

Enhancing Potentiometric Response of Electrochemical Sensor Using Modified Ion-Sensitive Transistor

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