Commercially‐Prepared Allograft Material Has Biological Activity In Vitro

Shigeyama, Yoichiro; D'Errico, John A.; Stone, R. T.; Somerman, Martha J.

doi:10.1902/jop.1995.66.6.478

Cited by 124 publications

(76 citation statements)

References 52 publications

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“…37,38 The terminal sterilization procedure by ethylene oxide may reduce osteoinductivity as well. 39 Another important factor to explain the lack of osteoinduction is the DBM particle size.…”

Section: Discussionmentioning

confidence: 99%

Mineralization processes in demineralized bone matrix grafts in human maxillary sinus floor elevations

Groeneveld

Bergh

Holzmann

et al. 1999

J. Biomed. Mater. Res.

106

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For reconstruction of the severely resorbed lateral maxilla for dental implant placement, one of the successful procedures is to elevate the maxillary sinus floor by implanting demineralized bone matrix (DBM). We studied bone formation in DBM grafts in the lateral maxilla in humans by means of histology and histomorphometry. Six months after grafting, at the time of dental implantation biopsies were taken from the grafted areas of seven patients. All biopsies contained mineralized matrix (MM) in the grafted area. At close inspection, three types of mineralization were found. First, lamellar biomineralization was seen in and near the maxillary host bone. Second, remineralization was observed in some particles that probably had not been completely demineralized. In the area connecting the graft and host bone, where woven bone was formed against DBM particles, a third mechanism was detected. In this case many dotlike foci of remineralization appeared close to the bone-DBM interface. The remineralized DBM and woven bone were both subsequently remodeled. Bone formation was most active in the area adjoining the maxillary host bone. We conclude that in human sinus floor elevation, allogenic DBM increases mineralized tissue volume by osteoconduction that is supported by the remineralization processes. Osteoinduction by this material seems questionable.

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Section: Discussionmentioning

confidence: 99%

Mineralization processes in demineralized bone matrix grafts in human maxillary sinus floor elevations

Groeneveld

Bergh

Holzmann

et al. 1999

J. Biomed. Mater. Res.

106

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“…As seen in Figure 7, DU145 cells expressed mRNAs for ␣v and ␤3. Given that OPN, a known ligand for ␣v␤3, is present in CBE [11], and that OPN promotes cell migration [17], we evaluated the ability of OPN to pro- Fig. 3.…”

Section: Du145 Cell Chemotaxismentioning

confidence: 99%

Effect of bone proteins on human prostate cancer cell lines in vitro

et al. 1998

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BACKGROUND. Despite the high incidence and serious consequences of skeletal metastasis in prostate cancer patients, the mechanisms involved in establishing secondary lesions in bone are not well-understood. In this study, the role of the mineralized bone matrix in the process of skeletal metastasis was evaluated. METHODS. Attachment, migration, and proliferation responses of human prostate cancer cells to a crude bone protein extract (CBE) were studied. LNCaP and DU145 cells were utilized in 24-hr attachment assays. Boyden chamber chemotactic assays and cell proliferation assays utilized DU145 cells. RESULTS. CBE and fibronectin (FN) promoted attachment of DU145 cells, whereas only FN facilitated attachment of LNCaP cells. CBE-mediated adhesion of DU145 cells was reduced by 94% with cycloheximide, by 98% with RGD peptides, and by 94% with an antibody to ␣v␤3. Although DU145 cells migrated toward FN, CBE did not promote migration of DU145 cells. DU145 cells grown in the presence of CBE-containing media demonstrated a significant reduction in cell number by day 4. The antiproliferative effect of CBE was not due to cell toxicity. CONCLUSIONS. In conclusion, results from this study indicate that mineralized bone proteins promote the attachment of DU145 cells in vitro and suggest that bone proteins may play a key role in vivo during the development of metastatic prostate lesions in bone.

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“…The current use of DBM is based on its purported osteoinductive ability [20]. Clinical use of DBM in revision arthroplasty has seldom been tried [5].…”

Section: Discussionmentioning

confidence: 99%

Restoration of bone defect and enhancement of bone ingrowth using partially demineralized bone matrix and marrow stromal cells

Shih

Sung

et al. 2005

J. Orthop. Res.

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Purpose: This study aimed to investigate the capability of combining marrow stromal cells (MSC) and partially demineralized bone matrix (PDBM) to fill bone defect and enhance bone ingrowth using a canine non-weight-bearing gap model.Methods: Custom-made implants with 3 mm gap between the porous surface and the host bone were used. The implants were inserted into the distal femurs of 25 mongrel dogs and the gaps were randomly assigned to be filled with culture-expanded autologous MSC-loaded PDBM, autograft, fresh-frozen allograft, PDBM alone, or nothing as controls. Histomorphometry using backscattered scanning electron microscopic examination, and mechanical push-out test were performed at 6 months after surgery.Results: Histomorphometry showed that amounts of bone regeneration in the gap and bone ingrowth into the porous-coated surface in the MSC-loaded PDBM-treated group were comparable to those of autograft-treated group and were significantly greater than those of allograft-treated, PDBM-treated, or non-grafted groups. Mechanical test showed the same differences. Conclusion:The results of this study showed that combining PDBM and autologous culture-expanded MSC restored bone stock and enhanced bone ingrowth into the porous-coated area in a canine non-weight-bearing gap model. This combination may provide an option for reconstructing bone defect when we perform a cementless revision arthroplasty.

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Commercially‐Prepared Allograft Material Has Biological Activity In Vitro

Cited by 124 publications

References 52 publications

Mineralization processes in demineralized bone matrix grafts in human maxillary sinus floor elevations

Mineralization processes in demineralized bone matrix grafts in human maxillary sinus floor elevations

Effect of bone proteins on human prostate cancer cell lines in vitro

Restoration of bone defect and enhancement of bone ingrowth using partially demineralized bone matrix and marrow stromal cells

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