Common 677C→T mutation of the 5,10-methylenetetrahydrofolate reductase gene affects follicular estradiol synthesis

Hecht, S.; Pavlik, R.; Lohse, Peter; Noss, U.; Friese, Klaus; Thaler, Christian J.

doi:10.1016/j.fertnstert.2007.11.011

Cited by 35 publications

(35 citation statements)

References 15 publications

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“…This possibility is supported by our finding that the rate of metaphase II oocytes did not differ among patients with the three genotypes ( Table 1). The concept of MTHFR activity affecting folliculogenesis is supported by previous reports by us (34,35) and other groups (36), showing reduced ovarian responsiveness and lower numbers of oocytes retrieved in IVF-ICSI patients with reduced MTHFR activity caused by genetic variations of the respective gene. In our logistic regression model, the MTHFR 677TT genotype also was identified as having a negative effect on oocyte numbers.…”

Section: Discussionsupporting

confidence: 53%

Divergent effects of the 677C>T mutation of the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene on ovarian responsiveness and anti-Müllerian hormone concentrations

Pavlik

Hecht

Ochsenkühn

et al. 2011

Fertility and Sterility

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Section: Discussionsupporting

confidence: 53%

Divergent effects of the 677C>T mutation of the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene on ovarian responsiveness and anti-Müllerian hormone concentrations

Pavlik

Hecht

Ochsenkühn

et al. 2011

Fertility and Sterility

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“…The remaining follicular aspirates were pooled. Isolation of GC using Percoll density gradient was carried out as described previously (Hecht et al 2009). In brief, after centrifugation at 1,000g for 10 min, the pellet consisting of GC, leukocytes, erythrocytes, macrophages and endothelial cells was diluted in PBS (phosphate buffered saline) and layered on Percoll (66%) in PBS and centrifuged for 10 min at 2,000g.…”

Section: Isolation Of Human Luteinized Gc and In Vitro Culturementioning

confidence: 99%

Induction of G protein-coupled estrogen receptor (GPER) and nuclear steroid hormone receptors by gonadotropins in human granulosa cells

Pavlik

Wypior

Hecht

et al. 2011

Histochem Cell Biol

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Estradiol and progesterone mediate their actions by binding to classical nuclear receptors, estrogen receptor α (ERα) and estrogen receptor β (ERβ) and progesterone receptor A and B (PR-A and PR-B) and the non-classical G protein-coupled estrogen receptor (GPER). Several animal knock-out models have shown the importance of the receptors for growth of the oocyte and ovulation. The aim of our study was to identify GPER in human granulosa cells (GC) for the first time. Moreover, the effect of different doses of gonadotropins on estrogen and progesterone receptors in the human ovary should be investigated as follicle stimulating hormone (FSH) and luteinizing hormone (LH) are also responsible for numerous mechanisms in the ovary like induction of the steroid biosynthesis. Human GC were cultured in vitro and stimulated with different doses of recombinant human FSH or LH. Receptor expression was analyzed by immunocytochemistry and quantitative real-time RT-PCR. GPER could be identified for the first time in human GC. It could be shown that high concentrations of LH increase GPER protein expression. Furthermore FSH and LH increased ERβ, PR-A and PR-B significantly on protein level. These findings were verified for high doses of FSH and LH on mRNA level. ERα was not affected with FSH or LH. We assume that gonadotropins induce GPER, ERβ and PR in luteinized granulosa cells.

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“…A correlation between methylenetetrahydrofolate reductase (MTHFR) C677 T mutation and a lower ovarian responsiveness (5) has been found. A possible connection between inherited thrombophilia and the etiology of infertility has been found in some studies (5)(6)(7)(8).…”

mentioning

confidence: 91%

Inherited thrombophilia in infertile women: implication in unexplained infertility

Casadei

Puca

Privitera

et al. 2010

Fertility and Sterility

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Common 677C→T mutation of the 5,10-methylenetetrahydrofolate reductase gene affects follicular estradiol synthesis

Cited by 35 publications

References 15 publications

Divergent effects of the 677C>T mutation of the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene on ovarian responsiveness and anti-Müllerian hormone concentrations

Divergent effects of the 677C>T mutation of the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene on ovarian responsiveness and anti-Müllerian hormone concentrations

Induction of G protein-coupled estrogen receptor (GPER) and nuclear steroid hormone receptors by gonadotropins in human granulosa cells

Inherited thrombophilia in infertile women: implication in unexplained infertility

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