Common antigens and male-female recognition in plants

Clarke, Adrienne E.; Harrison, S.; Knox, R. B.; Raff, J. W.; Smith, Patricia M.; Marchalonis, John J.

doi:10.1038/265161a0

Cited by 19 publications

(2 citation statements)

References 9 publications

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“…Support for this opinion comes from recent studies on anther-specific genes (11,18,20,23) of mRNAs in lily, tobacco, and rice pollen (25,30). There is also a similar literature on organ-and tissue-specific proteins in Japanese morning glory (2,22), Tulipa (1,3), Gladiolus pollen and stigma (7), Lycopersicon flowers (28), Petunia anthers (24), carrot stamen (17), maize and tobacco pollen (8,35), and, most recently, of developing lily (Lilium longiflorum) anthers (33).…”

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Immunological Characterization of a Tapetal Protein in Developing Anthers of Lilium longiflorum

Wang¹,

Walling²,

Eckard³

et al. 1992

Plant Physiol.

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Antiserum was raised in rabbits against a lily (Lilium longiflorum) anther-specific protein (LLA-15). Monospecific anti-LLA-15 antibodies were prepared to investigate the distribution of LLA-15 during anther development in a variety of flowering plants. Immunoblot analyses of total protein from floral and vegetative organs confirmed that LLA-15 or LLA-15-like proteins accumulated to detectable levels only in a discrete stage of anther development. In situ localization using anti-rabbit immunoglobulin G conjugated with gold particles confirmed that LLA-15 was specifically localized in the tapetal tissue of lily anthers. The maximal level of LLA-15 was strictly coincident with the peak of tapetal secretory functions.

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confidence: 83%

“…Ag/mL L-lysine, 0.4% ampholines, pH [3][4][5][6][7][8][9][10] [Serva], and 1.6% ampholines, pH [5][6][7] [Serva]) at 450C for 5 min (33). Gels were stained, destained, impregnated with Amplify (Amersham) for 30 min, and dried before fluorography.…”

Section: In Vitro Translation and Immunoprecipitationmentioning

confidence: 99%

Immunological Characterization of a Tapetal Protein in Developing Anthers of Lilium longiflorum

Wang¹,

Walling²,

Eckard³

et al. 1992

Plant Physiol.

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Characterization of an anther‐specific glycoprotein in Lilium longiflorum

Wang¹,

Walling²,

Eckard³

et al. 1993

American J of Botany

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Antiserum was raised in rabbits against a lily (Lilium longiflorum) anther‐specific glycoprotein (LLA‐75). LLA‐75 protein bound to concanavalin A, suggesting that it was a glycoprotein. Monospecific anti‐LLA‐75 antibodies were prepared to investigate its distribution during anther development. Immunoblot analyses of total protein from floral and vegetative organs show that LLA‐75 glycoproteins accumulated to detectable levels primarily in one discrete stage of anther development, during the microspore and early pollen phase when the tapetum is actively secreting. A cross‐reactive protein with molecular mass of 43.0 kD was also observed. Immunoblots of two‐dimensional polyacrylamide gels of lily anther proteins indicated that the four isoforms of LLA‐75 glycoprotein ranged from isoelectric point 5.6 to 6.1. These affinity purified antibodies to LLA‐75 cross‐reacted with anther proteins in two dicot species of the Bignoniaceae. In situ localization data using antirabbit immunoglobulin G (IgG) conjugated with gold particles was not definitive but demonstrated that LLA‐75 glycoprotein in lily anthers occurred prominently in tapetal tissue and all other tissues to a lesser degree.

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Discrimination of Self and Non-self in Plants

Knox

Clarke

1980

Contemporary Topics in Immunobiology

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Common antigens and male-female recognition in plants

Cited by 19 publications

References 9 publications

Immunological Characterization of a Tapetal Protein in Developing Anthers of Lilium longiflorum

Immunological Characterization of a Tapetal Protein in Developing Anthers of Lilium longiflorum

Characterization of an anther‐specific glycoprotein in Lilium longiflorum

Discrimination of Self and Non-self in Plants

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