Common Antigens in Oncornaviruses from Continuous Human Cell Lines and Mason-Pfizer Virus

Irlin, I. S.; Ilyin, K. V.; Bykovsky, A. F.; Miller, G. G.; Volkova, M.Ya.; Lozinsky, T. F.; Zhdanov, V. M.

doi:10.1159/000149411

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…The results have been inconclusive. A number of established human cell lines were found to harbor MPMV or other type D viruses (11,18,24,25,30,36,37,46,47), but these infections were usually ascribed to laboratory contaminations (30, 47). Other investigators have described MPMVreactive antisera from normal humans (22,35,45) and those with AIDS-related or non-AIDS-related lymphadenopathy (34).…”

Section: Discussionmentioning

confidence: 99%

“…The identification of AIDS-related lymphoma-derived viral isolates highly similar to a prototype simian type D retrovirus raised the possibility that these viruses might be laboratory contaminants. Previous investigators have observed MPMV or MPMV-related viruses in established human cell lines (18,24,25,30,36,37,46,47). Although we identified the virus in early passages of the cultured lymphoma cells and our laboratories have never worked with monkeys, simian retroviruses, or known MPMV-infected human cell lines, the possibility of laboratory contamination could only be ruled out by direct demonstration of the virus in the patient from whom the lymphomas were obtained.…”

Section: Tatggatgac V L P Q G M a N S P T L C Q K Y V A T A I H K V Rmentioning

confidence: 99%

“…However, a number of isolated reports have appeared over the last 15 years describing serological reactivity of human sera to type D virus antigens (22,34,35,44,45). In addition, a number of established human cell lines producing type D retroviruses have been described (11,16,24,25,37,47 cell line-derived type D isolates have been physically mapped with restriction endonucleases, and at least two of them have been molecularly cloned and sequenced. One cloned isolate was closely related to SRV-1 (30), and the other was very similar to the squirrel monkey retrovirus (36).…”

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confidence: 99%

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Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS

Bohannon

Donehower

Ford

1991

J Virol

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An atypical syncytial variant of a high-grade Burkitt's-type B-cell lymphoma from a patient with AIDS who was seropositive for human immunodeficiency virus type 1 was studied. A productive type D retrovirus infection was identified in early-passage cell lines derived from two lymphomas from this patient. Nucleotide and amino acid sequence analysis as well as immunologic reactivity indicated that the isolated virus was highly related to Mason-Pfizer monkey virus (MPMV). MPMV is an immunosuppressive type D retrovirus that causes an AIDS-like syndrome in rhesus macaques. Amplification of DNA from the patient's diagnostic bone marrow biopsy specimen by polymerase chain reaction generated the appropriate MPMV-specific fragments and indicated that the patient was infected with the MPMV-like retrovirus. In addition, the patient's serum contained antibodies which recognized type D viral env proteins (gp7O and gp2O) and gag proteins (p27 and p14). Although there have been reports of human cell lines infected with type D retroviruses and of type D-reactive human sera, this is the first evidence of a type D retrovirus infection in a human confirmed by virus isolation, serum reactivity, and viral DNA identification in tumor tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Tatggatgac V L P Q G M a N S P T L C Q K Y V A T A I H K V Rmentioning

confidence: 99%

mentioning

confidence: 99%

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Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS

Bohannon

Donehower

Ford

1991

J Virol

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Competition radioimmunoassay for mason‐pfizer monkey virus: Comparison with recent isolates

Yeh

Ahmed

Lyles

et al. 1975

Intl Journal of Cancer

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The major core protein of Mason-Pfizer monkey virus was purified by DEAE ion exchange column chromatography and shown to be 27,000 daltons (p27). Following the characterization of monospecific antisera prepared against p27, a radioimmunoassay was developed with these reagents and competition experiments were done with come of the recent M-PMY-like isolates as well as with other oncornaviruses. Results suggest that three of the viruses tested, AO, X-381 and FTP-1, are similar to M-PMV while J-96 virus is related, but not identical, to M-PMV. It is also shown that competition RIA can be used successfully to detect the presence of viral proteins in tissue homogenates and cell extracts.

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Identification of human type D retrovirus as a contaminant in a neuroblastoma cell line

Asikainen

Vesanen

Kuittinen

et al. 1993

Archives of Virology

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In this report we describe a type D virus isolated from a human neuroblastoma cell line (Paju). The viral RNA was isolated, partially molecularly cloned and sequenced. Our clones were shown to be identical to a human D-type retrovirus previously isolated from a human lymphoblastoid cell line. However, we obtained no evidence for the virus in earlier passage of the Paju cell line and therefore we must consider this isolate a laboratory contamination. contamination.

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Common Antigens in Oncornaviruses from Continuous Human Cell Lines and Mason-Pfizer Virus

Abstract: The oncornaviruses isolated from continuous human cell lines and the Mason-Pfizer virus share a common group-specific antigen that is revealed in the immunodiffusion test. They have no common antigens with the mouse mammary tumor virus.

Cited by 5 publications

References 3 publications

Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS

Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS

Competition radioimmunoassay for mason‐pfizer monkey virus: Comparison with recent isolates

Identification of human type D retrovirus as a contaminant in a neuroblastoma cell line

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