Common configurations and challenges in screen-printed enzymatic electrochemical biosensors

Walter, Piotr; Podsiadły, Bartłomiej; Wałpuski, Bartłomiej; Jakubowska, М.

doi:10.1117/12.2501724

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…Among the various types of electrochemical biosensors, enzyme-based variants utilizing screen-printed electrodes have attracted significant attention from researchers due to their high specificity, portability, low cost, and potential for mass production through miniaturization [3]. Enzymes, serving as the sensing unit, need to possess high sensitivity toward the target analyte, as well as long-time stability and strong binding to the receptor/working electrode [4,5]. Therefore, employing effective enzyme immobilization strategies is crucial in enhancing a biosensor's performance [6].…”

Section: Introductionmentioning

confidence: 99%

Enzyme Immobilization by Inkjet Printing on Reagentless Biosensors for Electrochemical Phosphate Detection

Zhang,

Bai,

Niu

et al. 2024

Biosensors

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Enzyme-based biosensors commonly utilize the drop-casting method for their surface modification. However, the drawbacks of this technique, such as low reproducibility, coffee ring effects, and challenges in mass production, hinder its application. To overcome these limitations, we propose a novel surface functionalization strategy of enzyme crosslinking via inkjet printing for reagentless enzyme-based biosensors. This method includes printing three functional layers onto a screen-printed electrode: the enzyme layer, crosslinking layer, and protective layer. Nanomaterials and substrates are preloaded together during our inkjet printing. Inkjet-printed electrodes feature a uniform enzyme deposition, ensuring high reproducibility and superior electrochemical performance compared to traditional drop-casted ones. The resultant biosensors display high sensitivity, as well as a broad linear response in the physiological range of the serum phosphate. This enzyme crosslinking method has the potential to extend into various enzyme-based biosensors through altering functional layer components.

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Section: Introductionmentioning

confidence: 99%

Enzyme Immobilization by Inkjet Printing on Reagentless Biosensors for Electrochemical Phosphate Detection

Zhang,

Bai,

Niu

et al. 2024

Biosensors

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“…Enzymatic biosensors have been studied and commercialized for decades and use enzymes as the chemically selective component to improve the selectivity of electrodes; bioelectrochemical methods using such bioelectrodes include potentiometric, amperometric, voltammetric, coulometric, and impedimetric techniques. While these bioelectrodes are typically used to determine the concentration of an analyte or an enzyme substrate in a particular sample (e. g., water, blood, urine, serum, sweat, etc.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Bioelectrocatalysis for Enzymology Applications

Kummer

2020

ChemElectroChem

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Enzymatic biosensors are commonly used to evaluate the concentration of individual analytes in samples (e. g., glucose in blood, lactate in sweat, etc.), where the working electrode utilizes an enzyme (a "bioelectrode") as the chemically selective agent for electrochemical analyses. However, those same enzymatic bioelectrodes can be used for the analytical characterization of enzymes as a new biophysical characterization tool for enzymologists. This Minireview will detail recent work focused on utilizing enzymatic bioelectrocataysis for enzymology applications. Specifically, it will talk about the use of enzymatic bioelectrodes for studying kinetics, inhibition, transport, and thermodynamics. It will also compare and contrast electrochemical techniques with spectrophotometric techniques for enzymology.[a] M.

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Common configurations and challenges in screen-printed enzymatic electrochemical biosensors

Cited by 2 publications

References 14 publications

Enzyme Immobilization by Inkjet Printing on Reagentless Biosensors for Electrochemical Phosphate Detection

Enzyme Immobilization by Inkjet Printing on Reagentless Biosensors for Electrochemical Phosphate Detection

Enzymatic Bioelectrocatalysis for Enzymology Applications

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