Common defects of different retinoic acid resistant promyelocytic leukemia cells are persistent telomerase activity and nuclear body disorganization

Nason-Burchenal, Kathryn; Maerz, Wolfgang J.; Albanell, Juan; Allopenna, Janet; Martin, Patrick; Moore, Malcolm A.S.; Dmitrovsky, Ethan

doi:10.1046/j.1432-0436.1997.6150321.x

Cited by 40 publications

(24 citation statements)

References 36 publications

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“…RA treatment repressed PML͞RAR␣ expression in NB4-S1, but not in NB4-R1 cells (13). To examine the relationship in APL cells between UBE1L and PML͞RAR␣ expression, immunoblot expression profiles for these species were examined before and after RA (1 M) treatment of NB4-S1 cells.…”

Section: Resultsmentioning

confidence: 99%

“…The NB4 APL cell line expresses PML͞RAR␣ (12). NB4-S1 and NB4-R1 are RA-sensitive and RA-resistant clones of NB4 cells, respectively (13). These cells were cultured in RPMI 1640 media supplemented with 10% FBS as described (13).…”

Section: Methodsmentioning

confidence: 99%

“…NB4-S1 and NB4-R1 are RA-sensitive and RA-resistant clones of NB4 cells, respectively (13). These cells were cultured in RPMI 1640 media supplemented with 10% FBS as described (13). Chinese hamster ovary (CHO) cells were cultured in ␣-MEM supplemented with 5% FBS, 100 units͞ml penicillin, 100 units͞ml streptomycin, and 2 mM L-glutamine in a 5% CO 2 humidified incubator at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…NB4 cell differentiation was scored by using the nitroblue tetrazolium (NBT) reduction assay (13,16,17). Transductants were identified by green fluorescent protein (GFP) coexpression.…”

Section: Methodsmentioning

confidence: 99%

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UBE1L is a retinoid target that triggers PML/RARα degradation and apoptosis in acute promyelocytic leukemia

Kitareewan

Pitha-Rowe

Sekula

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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114

127

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All-trans-retinoic acid (RA) treatment induces remissions in acute promyelocytic leukemia (APL) cases expressing the t(15;17) product, promyelocytic leukemia (PML)͞RA receptor ␣ (RAR␣). Microarray analyses previously revealed induction of UBE1L (ubiquitin-activating enzyme E1-like) after RA treatment of NB4 APL cells. We report here that this occurs within 3 h in RA-sensitive but not RA-resistant APL cells, implicating UBE1L as a direct retinoid target. A 1.3-kb fragment of the UBE1L promoter was capable of mediating transcriptional response to RA in a retinoid receptor-selective manner. PML͞RAR␣, a repressor of RA target genes, abolished this UBE1L promoter activity. A hallmark of retinoid response in APL is the proteasome-dependent PML͞RAR␣ degradation. UBE1L transfection triggered PML͞RAR␣ degradation, but transfection of a truncated UBE1L or E1 did not cause this degradation. A tight link was shown between UBE1L induction and PML͞RAR␣ degradation. Notably, retroviral expression of UBE1L rapidly induced apoptosis in NB4 APL cells, but not in cells lacking PML͞RAR␣ expression. UBE1L has been implicated directly in retinoid effects in APL and may be targeted for repression by PML͞ RAR␣. UBE1L is proposed as a direct pharmacological target that overcomes oncogenic effects of PML͞RAR␣ by triggering its degradation and signaling apoptosis in APL cells.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…NB4 cell differentiation was scored by using the nitroblue tetrazolium (NBT) reduction assay (13,16,17). Transductants were identified by green fluorescent protein (GFP) coexpression.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

UBE1L is a retinoid target that triggers PML/RARα degradation and apoptosis in acute promyelocytic leukemia

Kitareewan

Pitha-Rowe

Sekula

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

114

127

View full text Add to dashboard Cite

show abstract

“…3 RA-resistant cells have acquired specific defects including persistent telomerase activity, sustained PML oncogenic domain (POD) disorganization, mutations in the ligand-binding domain of the RARa portion of PML/RARa, or deregulation of RA target genes, among other changes. 3,30,31 Other myeloid leukemic cell lines exist that do not express PML/RARa but are retinoid responsive, such as the HL60 or U937 lines. The role of PML/RARa in mediating RA response in these and other cell contexts has been studied through PML/RARa transfection studies.…”

Section: Apl Modelsmentioning

confidence: 99%

Retinoid target genes in acute promyelocytic leukemia

et al. 2003

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Differential display as an approach to study differentiation and differentiation therapy in AML

Mills

2000

Hematol. Oncol.

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Common defects of different retinoic acid resistant promyelocytic leukemia cells are persistent telomerase activity and nuclear body disorganization

Cited by 40 publications

References 36 publications

UBE1L is a retinoid target that triggers PML/RARα degradation and apoptosis in acute promyelocytic leukemia

UBE1L is a retinoid target that triggers PML/RARα degradation and apoptosis in acute promyelocytic leukemia

Retinoid target genes in acute promyelocytic leukemia

Differential display as an approach to study differentiation and differentiation therapy in AML

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