Common European harmful algal blooms affect the viability and innate immune responses of Mytilus edulis larvae

Rijcke, Maarten De; Vandegehuchte, M.; Bussche, Julie Vanden; Nevejan, Nancy; Vanhaecke, Lynn; Schamphelaere, Karel A.C. De; Janssen, Colin R.

doi:10.1016/j.fsi.2015.09.003

Cited by 20 publications

(11 citation statements)

References 64 publications

(85 reference statements)

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“…However, it remains unclear whether this immune response is automatically triggered by abiotic factors or whether there is an opportunistic attack of pathogens present in the microbiota of the mussels. Current efforts are directed to clarify this question ( De Rijcke et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Unbiased high-throughput characterization of mussel transcriptomic responses to sublethal concentrations of the biotoxin okadaic acid

Suárez-Ulloa

Fernández‐Tajes

Aguiar‐Pulido

et al. 2015

PeerJ

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Background. Harmful Algal Blooms (HABs) responsible for Diarrhetic Shellfish Poisoning (DSP) represent a major threat for human consumers of shellfish. The biotoxin Okadaic Acid (OA), a well-known phosphatase inhibitor and tumor promoter, is the primary cause of acute DSP intoxications. Although several studies have described the molecular effects of high OA concentrations on sentinel organisms (e.g., bivalve molluscs), the effect of prolonged exposures to low (sublethal) OA concentrations is still unknown. In order to fill this gap, this work combines Next-Generation sequencing and custom-made microarray technologies to develop an unbiased characterization of the transcriptomic response of mussels during early stages of a DSP bloom.Methods. Mussel specimens were exposed to a HAB episode simulating an early stage DSP bloom (200 cells/L of the dinoflagellate Prorocentrum lima for 24 h). The unbiased characterization of the transcriptomic responses triggered by OA was carried out using two complementary methods of cDNA library preparation: normalized and Suppression Subtractive Hybridization (SSH). Libraries were sequenced and read datasets were mapped to Gene Ontology and KEGG databases. A custom-made oligonucleotide microarray was developed based on these data, completing the expression analysis of digestive gland and gill tissues.Results. Our findings show that exposure to sublethal concentrations of OA is enough to induce gene expression modifications in the mussel Mytilus. Transcriptomic analyses revealed an increase in proteasomal activity, molecular transport, cell cycle regulation, energy production and immune activity in mussels. Oppositely, a number of transcripts hypothesized to be responsive to OA (notably the Serine/Threonine phosphatases PP1 and PP2A) failed to show substantial modifications. Both digestive gland and gill tissues responded similarly to OA, although expression modifications were more dramatic in the former, supporting the choice of this tissue for future biomonitoring studies.Discussion. Exposure to OA concentrations within legal limits for safe consumption of shellfish is enough to disrupt important cellular processes in mussels, eliciting sharp transcriptional changes as a result. By combining the study of cDNA libraries and a custom-made OA-specific microarray, our work provides a comprehensive characterization of the OA-specific transcriptome, improving the accuracy of the analysis of expresion profiles compared to single-replicated RNA-seq methods. The combination of our data with related studies helps understanding the molecular mechanisms underlying molecular responses to DSP episodes in marine organisms, providing useful information to develop a new generation of tools for the monitoring of OA pollution.

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Section: Discussionmentioning

confidence: 99%

Unbiased high-throughput characterization of mussel transcriptomic responses to sublethal concentrations of the biotoxin okadaic acid

Suárez-Ulloa

Fernández‐Tajes

Aguiar‐Pulido

et al. 2015

PeerJ

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“…Regarding marine resources, dissolved DA is reported to alter bivalve larvae growth (Liu et al, 2007) and immunity (De Rijcke et al, 2015) and these effects could spread to population recruitment and picking activities. Dissolved DA is also reported to inhibit herbivorous feeding as shown for krill (Bargu et al, 2006) and copepod species (Tammilehto et al, 2015) modifying matter and energy fluxes within the trophic web.…”

Section: Da Implications In the Ecosystem And Challenges For Future Smentioning

confidence: 99%

Pseudo-nitzschia sp. diversity and seasonality in the southern North Sea, domoic acid levels and associated phytoplankton communities

Delegrange

Lefebvre

Gohin

et al. 2018

Estuarine, Coastal and Shelf Science

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The diversity, toxicity and seasonality of Pseudo-nitzschia sp. were investigated from February to November 2012 in the southern Bight of the North Sea (SBNS) along the French coast. The identification of Pseudo-nitzschia species in this area was addressed for the first time in this study. Our results revealed a low species richness (3 distinct species) in association with moderate (102 pg mL −1) to high (263 pg mL −1) domoic acid (DA) levels in autumn and spring, respectively. Pseudo-nitzschia succession corresponded to the dominance of P. delicatissima in April-May (86% of total diatoms) as a co-occurring species of the Phaeocystis globosa bloom. Following the Phaeocystis bloom (May-September), P. pungens dominated markedly over P. fraudulenta and P. delicatissima and was the only species present in autumn, although at low abundance (<1000 cell L −1). The results of this study support the idea that Pseudo-nitzschia seasonality in the SBNS relies principally on temperature and nutrient availability (DIN and silicates), which, in turn, depend on locally fluctuating environmental conditions (rainfalls and winds). This study highlights the potential for the SBNS to be a potential risk area in regard to the possible impacts of DA on marine resources and the DA transfer through marine food webs. This is of particular concern since DA concentration in seawater was not systematically correlated to potentially toxic Pseudo-nitzschia abundance. Highlights ► Low Pseudo-nitzschia sp. diversity (three species) in the southern North Sea. ► Domoic acid levels reached 263 pg mL −1 in May 2012, 83% as dissolved DA. ► Strong species seasonality driven by temperature and nutrient availability (DIN and silicates). ► Autumnal DA concentrations could be attributed to P. pungens. ► DA concentration was not systematically correlated to Pseudo-nitzschia spp. abundance.

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“…However, most previous research focused on a single variant or artificial mixture of MCs, which could have different degradation dynamics and toxicity from natural MC mixture [20]. Therefore, there is a critical need for developing more environmentally-friendly and effective methods for controlling MC contamination to protect both public health and water/food safety, especially because harmful algal blooms are expected to be more frequent and intensive globally [21,22]. …”

Section: Introductionmentioning

confidence: 99%

Sustainable Methods for Decontamination of Microcystin in Water Using Cold Plasma and UV with Reusable TiO2 Nanoparticle Coating

Jiang

Lee

Mok

et al. 2017

IJERPH

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Microcystins (MCs) are a family of cyanotoxins and pose detrimental effects on human, animal, and ecological health. Conventional water treatment processes have limited success in removing MCs without producing harmful byproducts. Therefore, there is an urgent need for cost-effective and environmentally-friendly methods for treating MCs. The objective of this study was to develop sustainable and non-chemical-based methods for controlling MCs, such as using cold plasma and ultra violet (UV) light with titanium dioxide (TiO2) coating, which can be applied for diverse scale and settings. MCs, extracted from Microcystis aeruginosa, were treated with cold plasma or UV at irradiance of 1470 μW/cm2 (high) or 180 μW/cm2 (low). To assess synergistic effects, the outside of the UV treatment chamber was coated with nanoparticles (TiO2) prior to irradiation, which can be reused for a long time. The degradation efficiency of UV was enhanced by the reusable TiO2 coating at lower irradiance (70.41% [UV] vs. 79.61% [UV+TiO2], 120 min), but no significant difference was observed at higher irradiance. Cold plasma removed MCs rapidly under experimental conditions (92%, 120 min), indicating that it is a promising candidate for controlling MCs in water without generating harmful disinfection byproducts. It can be also easily and practically used in household settings during emergency situations.

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Common European harmful algal blooms affect the viability and innate immune responses of Mytilus edulis larvae

Cited by 20 publications

References 64 publications

Unbiased high-throughput characterization of mussel transcriptomic responses to sublethal concentrations of the biotoxin okadaic acid

Unbiased high-throughput characterization of mussel transcriptomic responses to sublethal concentrations of the biotoxin okadaic acid

Pseudo-nitzschia sp. diversity and seasonality in the southern North Sea, domoic acid levels and associated phytoplankton communities

Sustainable Methods for Decontamination of Microcystin in Water Using Cold Plasma and UV with Reusable TiO2 Nanoparticle Coating

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