Common findings of bla
                     CTX-M-55-encoding 104–139 kbp plasmids harbored by extended-spectrum β-lactamase-producing Escherichia coli in pork meat, wholesale market workers, and patients with urinary tract infection in Vietnam

Hoang, T. A. V.; Nguyen, Trong Duc; Ueda, Shuhei; Le, Qu; Tran, Tam; Nguyen, Thang Nam; Dao, Trinh-Tam; Tran, Minhtien; Le, Thanh Thi; Le, T. L.; Nakayama, Tadasi; Hirai, Itaru; H., T.; Vien, Quang Mai; Yamamoto, Yoshimasa

doi:10.1007/s00284-016-1174-x

Cited by 30 publications

(25 citation statements)

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“…Eight of the MCR-3-producing Salmonella isolates from the Danish patients were also positive for bla CTX-M-55 . CTX-M-55 was first reported from Thailand [ 10 ], and the bla CTX-M-55 gene is a prevalent gene in Gram-negative bacteria isolated from animals and humans in Asia [ 11 - 13 ]. Furthermore, bla CTX-M-55 -positive monophasic Salmonella Typhimurium has been isolated from patients who reported travel to Thailand [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Plasmid-borne colistin resistance gene mcr-3 in Salmonella isolates from human infections, Denmark, 2009–17

et al. 2017

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This report describes one Salmonella isolate harbouring both mcr-1 and mcr-3. We also found nine other Salmonella isolates positive for the plasmid-borne colistin resistance gene, mcr-3. The strains were isolated from patients in Denmark between 2009 and 2017 and five of the patients had travelled to Asia. In addition to mcr-3, all strains were found positive for blaTEM-1, strA, strB, sul2 and tet(A) or tet(B), and most strains were positive for blaCTX-M-55 and qnrS.

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Section: Discussionmentioning

confidence: 99%

Plasmid-borne colistin resistance gene mcr-3 in Salmonella isolates from human infections, Denmark, 2009–17

et al. 2017

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“…Genotyping of ESBL-encoding genes and phylogenetic groupings were determined by multiplex polymerase chain reaction (PCR). 10 The presence of the colistin-resistant gene mcr-1 was also confirmed by PCR. 3 For both ESBL-encoding genes and mcr-1 , the PCR products were identified by sequencing.…”

Section: Methodsmentioning

confidence: 85%

“…Their genotyping results were similar to those of healthy Vietnamese residents in terms of bla CTX-M genotypes, such as bla CTX-M-1 and bla CTX-M-9 groups. 10 Conversely, an abundance of phylogenetic group D was observed in the isolates. However, the reason for this difference from healthy Vietnamese residents 10 was not clear.…”

Section: Discussionmentioning

confidence: 94%

“… 10 Conversely, an abundance of phylogenetic group D was observed in the isolates. However, the reason for this difference from healthy Vietnamese residents 10 was not clear. It may be due to differences in intestinal flora between Japanese travelers and Vietnamese residents.…”

Section: Discussionmentioning

confidence: 94%

“… 3 For both ESBL-encoding genes and mcr-1 , the PCR products were identified by sequencing. 3 , 10 In mcr-1 -positive strains, the colistin minimum inhibitory concentration (MIC) was evaluated by E-test ® (bioMérieux).…”

Section: Methodsmentioning

confidence: 99%

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Carriage of colistin-resistant, extended-spectrum β-lactamase-producing<em> Escherichia coli</em> harboring the <em>mcr-1</em> resistance gene after short-term international travel to Vietnam

Nakayama¹,

Kumeda²,

Kawahara³

et al. 2018

IDR

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BackgroundDue to increasing colistin usage, the dissemination of the colistin-resistant gene mcr-1 has been increasingly investigated. The aim of this study was to determine whether a traveler on a short-term international trip to a developing country could bring mcr-1 back to their home country.Materials and methodsThirty-four travel events from Japan to Vietnam encompassing 19 travelers were assessed. A fecal specimen was collected from each traveler before and after each travel event and was inoculated on CHROMagar containing cefotaxime (CTX). Three to seven colonies exhibiting the characteristics of Escherichia coli were collected. Susceptibility to antibiotics and extended-spectrum β-lactamase (ESBL) production were determined by the disk diffusion method and the double-disk synergy test, respectively. ESBL-encoding genes were genotyped, and phylogenetic groupings were determined by multiplex polymerase chain reaction (PCR). The presence of mcr-1 was also confirmed by PCR and sequencing.ResultsA total of 175 ESBL-producing E. coli isolated before and up to 2 weeks after traveling to Vietnam were analyzed. Genotyping of ESBL-producing isolates showed that blaCTX-M-1/blaTEM (27.7%) and blaCTX-M-9 (45.9%) were the most prevalent genotypes, while the most frequently detected phylogenetic group was D (41.9%) followed by B2 (23.0%). In a significant number of travel events, travelers brought ESBL-producing E. coli back to Japan and three events by three travelers carried mcr-1. ESBL-producing E. coli isolates harboring mcr-1 were identified as those carrying both blaCTX-M-14 or blaCTX-M-55 and mcr-1.ConclusionUsing Vietnam as an example, we have shown that even a short-term trip to some countries may result in ESBL-producing mcr-1-positive E. coli carriage by international travelers.

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Abundance of colistin-resistant Escherichia coli harbouring mcr-1 and extended-spectrum β-lactamase-producing E. coli co-harbouring blaCTX-M-55 or -65 with blaTEM isolates from chicken meat in Vietnam

Nakayama

Thi²,

Thanh³

et al. 2022

Arch Microbiol

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Although the spread of plasmid-mediated antibiotic-resistant bacteria is a public health concern, food contamination with plasmid-mediated antibiotic-resistant Escherichia coli has not been well investigated in Vietnam. The aim of this study was to describe the prevalence of colistinresistant, carbapenem-resistant and endemic bla CTX−M in extended-spectrum β-lactamase (ESBL)-producing E. coli isolates. Colistin-and carbapenemresistant ESBL-producing E. coli were isolated from chickens in Vietnam and Japan. The results showed that 52% and 93% of Vietnamese chicken was isolated with colistin-resistant and AmpC/ESBL-producing E. coli, respectively, while 52.7% of Japanese chickens were isolated with AmpC/ESBLproducing E. coli. Carbapenem-resistant E. coli has not been isolated in Vietnam or Japan. Genotyping revealed that colistin-resistant E. coli harboured mcr-1, and most of the AmpC/ESBL-related genes were bla CTX−M−55 and bla CTX−M−65 together with bla TEM in Vietnamese chickens, and bla CMY−2 in Japanese chickens. Multidrug resistance analysis showed that ESBL-producing E. coli isolates were more resistant to quinolones, streptomycin, and chloramphenicol compared with colistin-resistant E. coli isolates from Vietnam, suggesting selection in ESBL-producing E. coli for multiple antibiotic resistance genes. In conclusion, colistin-resistant E. coli was detected in about half of the chicken meat samples, the majority of which were found to harbour mcr-1. The high prevalence of ESBL-producing E. coli has remained constant across the last ve years, and the predominant bla CTX−M for ESBL-producing E. coli was found to be bla CTX−M−55 or bla CTX−M−65 , with the coexistence of bla TEM in Vietnam. Our results can be implemented in monitoring systems to combat the development of antimicrobial resistance.

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Common findings of bla CTX-M-55-encoding 104–139 kbp plasmids harbored by extended-spectrum β-lactamase-producing Escherichia coli in pork meat, wholesale market workers, and patients with urinary tract infection in Vietnam

Cited by 30 publications

References 32 publications

Plasmid-borne colistin resistance gene mcr-3 in Salmonella isolates from human infections, Denmark, 2009–17

Plasmid-borne colistin resistance gene mcr-3 in Salmonella isolates from human infections, Denmark, 2009–17

Carriage of colistin-resistant, extended-spectrum β-lactamase-producing<em> Escherichia coli</em> harboring the <em>mcr-1</em> resistance gene after short-term international travel to Vietnam

Abundance of colistin-resistant Escherichia coli harbouring mcr-1 and extended-spectrum β-lactamase-producing E. coli co-harbouring blaCTX-M-55 or -65 with blaTEM isolates from chicken meat in Vietnam

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Common findings of bla CTX-M-55-encoding 104–139 kbp plasmids harbored by extended-spectrum β-lactamase-producing Escherichia coli in pork meat, wholesale market workers, and patients with urinary tract infection in Vietnam

Cited by 30 publications

References 32 publications

Plasmid-borne colistin resistance gene mcr-3 in Salmonella isolates from human infections, Denmark, 2009–17

Plasmid-borne colistin resistance gene mcr-3 in Salmonella isolates from human infections, Denmark, 2009–17

Carriage of colistin-resistant, extended-spectrum &beta;-lactamase-producing<em> Escherichia coli</em> harboring the <em>mcr-1</em> resistance gene after short-term international travel to Vietnam

Abundance of colistin-resistant Escherichia coli harbouring mcr-1 and extended-spectrum β-lactamase-producing E. coli co-harbouring blaCTX-M-55 or -65 with blaTEM isolates from chicken meat in Vietnam

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Carriage of colistin-resistant, extended-spectrum β-lactamase-producing<em> Escherichia coli</em> harboring the <em>mcr-1</em> resistance gene after short-term international travel to Vietnam