In recent years, aquatic sports (AS) have become more popular, especially for those who enjoy the adventurous sports activities. However, the AS have the possibility of injury, although the activities are performed in the water media. This study aimed to determine the musculoskeletal injury profile and incidence among AS athletes in Sultan Idris Education University (UPSI) from 2018-2020. A total of 62 staff and students who are AS athletes participated in this study. The injuries of athletes were assessed based on the type, location and cause of injuries within the last two years of competition events or training sessions and through an online survey method by Sport Injury Reporting Questionnaire. The AS were swimming, kayaking, canoeing, dragon boating, and rowing. The study recorded 102 injury cases in total. The results showed muscle cramps were the most common injury, followed by abrasion contusion and muscle strain. The most common body location injury specifically in swimming was the shoulder injury, followed by forearm, hip and thigh. In addition, overuse and chronic were the highest injury causes, followed by poor paddling technique and low level of strength and only a few of the respondents reported a lack of proper warm-up and cool-down. These findings revealed the injuries probably occurred in the upper body, which was caused by overuse training and inappropriate technique, particularly in swimming strokes and boating. These findings may be useful when developing future training program that aims to prevent sport injuries in aquatic sports.