Common nuclear matrix proteins in rat tissues

Korosec, Thomas; Gerner, Christopher; Sauermann, Georg

doi:10.1002/elps.1150181138

Cited by 15 publications

(9 citation statements)

References 21 publications

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“…The complex molecular composition of the nuclear matrix was extensively studied (Berezney 1991;Mattern et al 1997); apparently, cells of different origin have a large set of matrix proteins in common (Korosec et al 1997). Lamins are a major component of the nuclear matrix/ nucleoskeleton (for a review, see Moir et al 1995) and are present in the nuclear lamina (Gerace and Blobel 1982) as well as in the nuclear interior (Hozµk et al 1995).…”

Section: Introductionmentioning

confidence: 98%

Nucleoskeleton of early bovine embryos and differentiated somatic cells: an ultrastructural and immunocytochemical comparison

Degrouard¹,

Hozák

Heyman³

et al. 2004

Histochem Cell Biol

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The nucleoskeleton is a complex structure involved in structural and functional organisation of the genome in eukaryotic cells. As little information on the nucleoskeleton is available from early embryonic development stages, we describe here the morphology and composition of the nucleoskeleton in cleaving bovine embryos (stages 1-16 cells). Ultrastructural observations were performed using thick resinless sections after chromatin removal by nucleases. The localisation of nucleoskeleton-related lamins A and C, NuMA, SRm160 and hnRNP H was tested by immunofluorescence. The characteristic structures of the nucleoskeleton (nuclear lamina, core filaments and the 'diffuse' nucleoskeleton) were present throughout all embryonic stages studied, although less discernable during the 1-cell stage. Lamins A and C as well as the NuMA protein were observed in embryo nuclei from the 1-cell stage; a diffuse hnRNP H and speckled SRm160 immunofluorescence appeared from the 4-cell stage. During the 8- to 16-cell stages (major transcriptional activation), the immunofluorescence patterns were identical with those of differentiated cells (fibroblasts). The temporal pattern of immunolabelling confirmed that transcription and splicing compartmentalisation was established progressively during cleavage, and that some of the proteins tested can be used as markers in studies on nuclei reprogramming after transfer into enucleated oocytes.

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Section: Introductionmentioning

confidence: 98%

Nucleoskeleton of early bovine embryos and differentiated somatic cells: an ultrastructural and immunocytochemical comparison

Degrouard¹,

Hozák

Heyman³

et al. 2004

Histochem Cell Biol

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“…The protein composition of the nuclear matrix is highly complex, with nearly a thousand individual spots identi ed in different rat tissues by two-dimensional electrophoresis and silver staining (2). This striking complexity is not surprising in view of the involvement of the nuclear matrix with many aspects of nuclear functioning.…”

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confidence: 99%

The Protein Composition of the Hepatocyte Nuclear Matrix Is Differentiation‐Stage Specific

Ivanović‐Matić¹,

Dinić²,

Vujošević³

et al. 2000

IUBMB Life

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SummaryThe protein composition of hepatocyte nuclear matrices was examined in rats from the 16th day of gestation to 75 days after birth (adult). An overall increase in size of the nuclear matrix was accompanied by quantitative and qualitative changes in its protein content. Quantitative changes of the major proteins of the peripheral lamina surrounding the isolated nuclear matrix were detected. By Western analysis we established that in pre-and postnatal nuclear matrices the relative concentrations of lamin C were greater than lamin A. After birth, the relative concentrations of both lamins progressively increased. In the adult nuclear matrix, the concentration of lamin A was greater than lamin C. In contrast, the relative concentrations of lamin B remained unchanged throughout development and growth. The relative concentrations of two nuclear matrix -associated regulatory proteins studied changed with development and growth: transcription factor C/EBP® isoforms, which were detected during the gestation period, increased notably after the rst postnatal day, attaining a maximum at the adult stage; the high concentrations of the proliferating cell nuclear antigen (PCNA) perceptibly decreased after the 21st prenatal day. Changes in the composition of the nuclear matrix protein suggest that this structure coordinates nuclear functioning during cell differentiation.IUBMB Life, 49: 511 -517, 2000 Keywords C/EBPa ; hepatocyte development; lamins; nuclear matrix; PCNA.The nuclear matrix is a proteinaceous structural framework of the nucleus. The isolated matrix has a tripartite structure and

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“…In our effort to classify nuclear matrix proteins, samples from various human and rat tissues, cells, and cell lines [Holzmann et al, 1997;Korosec et al, 1997;Gerner et al, 1998;Gotzmann et al, 1997] are presently being analyzed. In practice, application of a standardized procedure for preparation of nuclear matrix protein samples from materials divergent as, for example, bladder cancer tissue and myeloid cells was not applicable.…”

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confidence: 99%

Similarity between nuclear matrix proteins of various cells revealed by an improved isolation method

et al. 1998

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Comparative analysis of nuclear matrix proteins by two-dimensional electrophoresis may be greatly impaired by copurifying cytoskeletal proteins. The present data show that the bulk of adhering cytofilaments may mechanically be removed by shearing of nuclei pretreated with vanadyl ribonucleoside complexes. Potential mechanisms of action not based on ribonuclease inhibition are discussed. To individually preserve the integrity of nuclear structures, we developed protocols for the preparation of nuclear matrices from three categories of cells, namely leukocytes, cultured cells, and tissue cells. As exemplified with material from human lymphocytes, cultured amniotic cells, and liver tissue cells, the resulting patterns of nuclear matrix proteins appeared quite similar. Approximately 300 spots were shared among the cell types. Forty-nine of these were identified, 21 comprising heterogeneous nuclear ribonucleoproteins. Heterogeneous nuclear ribonucleoproteins L and nuclear lamin B2 isoforms were identified by amino acid sequencing and mass spectrometry. However, individually expressed proteins, such as the proliferating cell nuclear antigen, also pertained following application of the protocols. Thus, enhanced resolution and comparability of proteins improve systematic analyses of nuclear matrix proteins from various cellular sources.

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Common nuclear matrix proteins in rat tissues

Cited by 15 publications

References 21 publications

Nucleoskeleton of early bovine embryos and differentiated somatic cells: an ultrastructural and immunocytochemical comparison

Nucleoskeleton of early bovine embryos and differentiated somatic cells: an ultrastructural and immunocytochemical comparison

The Protein Composition of the Hepatocyte Nuclear Matrix Is Differentiation‐Stage Specific

Similarity between nuclear matrix proteins of various cells revealed by an improved isolation method

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