Communication. Fluorescamine and fluorescein as labels in energy-transfer immunoassay

Abstract

Material for publication as a Communication must be on a n urgent matter and be of obvious scientific importance. Rapidity of publication i s enhanced if diagrams are omitted, but tables and formulae can be included. Communications should not be simple claims for priority: this facility for rapid publication i s intended for brief descriptions of work that has progressed to a stage at which it is likely to be valuable to workers faced with similar problems. A fuller paper may be ojjfered subsequently, if justi,fied by later work.Manuscripts are not subjected to the usual examination by referees and inclusion of a Communication is at the Editor's discretion.

Fluorescence energy transfer methods in bioanalysis

“…Antigen-antibody binding can thus be detected without the need for a separation step. In early work fluorescein and rhodamine were used as the D-A pair, though other pairs of fluorophores were studied, 15 and a commercial version of the assay used a ''dark'' acceptor, 49,59-dimethoxy-fluorescein, with fluorescein itself as the donor. 16 Immunoassays could thus be carried out using a simple instrument optimised for the detection of fluorescein.…”

Radioiodination and Other Labeling Techniques

“…This method has been applied to both direct and indirect fluorescence quenching immunoassays (ULLMAN et al 1976;MILLER et al 1980;KHANNA and ULLMAN 1980;THAKRAR and MILLER 1982). Because the emission and absorption spectra of the two fluorescent compounds overlap, emission from the donor will be quenched by the acceptor.…”