Background: People from different cultural backgrounds have used different forms of Traditional Medicine as a means to managing their ailments. This study examined public perceptions of the role of Traditional Medicine in relation to the accessibility, conditions treated, safety, efficacy rate and interactions between traditional and orthodox medical systems in the Sekyere South District of Ashanti, Ghana. Methods: This was a descriptive cross-sectional survey. A sample of 70 Traditional Medical Practitioners, 30 health care users, and 20 Orthodox Medical Practitioners were used and selected respectively based on snowball, random sampling and purposive techniques. Both quantitative and qualitative data were collected through administered-questionnaire and in-depth interviews. Non-participant observation was used to assess the working environments of the practitioners in 9 selected communities in the district. Relevant data obtained were analysed using both qualitative and quantitative methods. Percentages and cross-tabulations, using the Predictive Analytic Software (PASW), version i6.0 were the methods used to analyse the data.