Community composition, bacterial symbionts, antibacterial and antioxidant activities of honeybee-associated fungi

Cui, Pu; Kong, Kun; Yao, Yong; Huang, Zhongdi; Shi, Shuping; Liu, Peng; Huang, Yechen; Abbas, Naeem; Yu, Linsheng; Zhang, Yinglao

doi:10.1186/s12866-022-02580-4

Cited by 12 publications

(11 citation statements)

References 69 publications

(72 reference statements)

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“…The relative abundances of ASVs showed data similar to ours at the phylum level, with a highest abundance of Ascomycota followed by a lower proportion of Basidiomycota. Data at genus level (Cui et al, 2022b) showed a relative abundance of Kodamaea higher than 80%, different from our results that recorded this genus at 8%, on the other hand, Metschnikowia was not detected at all (threshold 0.1%). The Metschnikowia genus was, on the contrary, detected at 18% of relative abundance in honeycomb in the same study.…”

Section: Discussioncontrasting

confidence: 99%

“…However, no isolation of the species detected in our work has been documented so far. A recent work by Cui et al (2022b) explored the phylogenetic diversity and community composition of A. mellifera ligustica associated fungi in honey bees and the colony environment, including the gut and bee-derived products using a combination of culture-dependent and cultureindependent approaches. The relative abundances of ASVs showed data similar to ours at the phylum level, with a highest abundance of Ascomycota followed by a lower proportion of Basidiomycota.…”

Section: Discussionmentioning

confidence: 99%

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Environment or genetic isolation? An atypical intestinal microbiota in the Maltese honey bee Apis mellifera spp. ruttneri

Gaggìa

Jakobsen²,

Alberoni³

et al. 2023

Front. Microbiol.

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IntroductionApis mellifera evolved mainly in African, Asian, and European continents over thousands of years, leading to the selection of a considerable number of honey bees subspecies that have adapted to various environments such as hot semi-desert zones and cold temperate zones. With the evolution of honey bee subspecies, it is possible that environmental conditions, food sources, and microbial communities typical of the colonized areas have shaped the honey bee gut microbiota.MethodsIn this study the microbiota of two distinct lineages (mitochondrial haplotypes) of bees Apis mellifera ruttneri (lineage A) and Apis mellifera ligustica and carnica (both lineage C) were compared. Honey bee guts were collected in a dry period in the respective breeding areas (the island of Malta and the regions of Emilia-Romagna and South Tyrol in Italy). Microbial DNA from the honey bee gut was extracted and amplified for the V3-V4 regions of the 16S rRNA gene for bacteria and for ITS2 for fungi.ResultsThe analyses carried out show that the Maltese lineage A honey bees have a distinctive microbiota when compared to Italian lineage C honey bees, with the most abundant genera being Bartonellaceae and Lactobacillaceae, respectively. Lactobacillaceae in Maltese Lineage A honey bees consist mainly of Apilactobacillus instead of Lactobacillus and Bombilactobacillus in the lineage C. Lineage A honey bee gut microbiota also harbors higher proportions of Arsenophonus, Bombella, Commensalibacter, and Pseudomonas when compared to lineage C.DiscussionThe environment seems to be the main driver in the acquisition of these marked differences in the gut microbiota. However, the influence of other factors such as host genetics, seasonality or geography may still play a significant role in the microbiome shaping, in synergy with the environmental aspects.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Environment or genetic isolation? An atypical intestinal microbiota in the Maltese honey bee Apis mellifera spp. ruttneri

Gaggìa

Jakobsen²,

Alberoni³

et al. 2023

Front. Microbiol.

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“…In addition to containing a large number of gut bacteria, the bee gut is rich in fungi, which have antibacterial and antioxidant activities, and probiotic potential ( Hsu et al, 2021 ; Cui et al, 2022 ; Rutkowski et al, 2022 ). However, the effects of pesticide exposure on the gut fungi of bees are often overlooked.…”

Section: Discussionmentioning

confidence: 99%

Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris

Tang

Wang

et al. 2023

Front. Physiol.

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The sublethal effects of pesticide poisoning will have significant negative impacts on the foraging and learning of bees and bumblebees, so it has received widespread attention. However, little is known about the physiological effects of sublethal spinetoram and glyphosate exposure on bumblebees. We continuously exposed Bombus terrestris to sublethal (2.5 mg/L) spinetoram or glyphosate under controlled conditions for 10 days. The superoxide dismutase, glutathione-S-transferase, carboxylesterase, prophenoloxidase, α-amylase and protease activities, and changes in gut microbes were measured to understand the effects of sublethal pesticide exposure on the physiology and gut microbes of bumblebees. Sublethal pesticide exposure to significantly increased superoxide dismutase activity and significantly decreased gut α-amylase activity in bumblebees but had no significant effect on glutathione-S-transferase, carboxylesterase or gut protease activities. In addition, glyphosate increased the activity of prophenoloxidase. Interestingly, we observed that neither of the two pesticides had a significant effect on dominant gut bacteria, but glyphosate significantly altered the structure of the dominant gut fungal community, and reduced the relative abundance of Zygosaccharomyces associated with fat accumulation. These results suggest that sublethal spinetoram and glyphosate do not significantly affect the detoxification system of bumblebees, but may affect bumblebee health by inhibiting energy acquisition. Our results provide information on the sublethal effects of exposure to low concentrations of glyphosate and spinetoram on bumblebees in terms of physiology and gut microbes.

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“…The PCR reaction was carried out with 15 μl of Phusion ® High-Fidelity PCR Master Mix (New England Biolabs), 2 μM of forward/reverse primers, and about 10 ng of template DNA. The reaction conditions of PCR were performed as described method (Cui et al, 2022). Mixture PCR products were purified with Qiagen Gel Extraction Kit (Qiagen, Germany).…”

Section: Culture-independent Community Analysismentioning

confidence: 99%

Diversity and antibacterial potential of the Actinobacteria associated with Apis mellifera ligustica

Cui

Jiang

et al. 2022

Front. Microbiol.

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Insect-associated Actinobacteria are a potentially rich source of novel natural products with antibacterial activity. Here, the community composition of Actinobacteria associated with Apis mellifera ligustica was investigated by integrated culture-dependent and independent methods. A total of 61 strains of Streptomyces genera were isolated from the honeycomb, larva, and different anatomical parts of the honeybee’s body using the culture-dependent method. Amplicon sequencing analyses revealed that the actinobacterial communities were dominated by the family of Bifidobacteriaceae and Microbacteriaceae in the honeybee gut, and Nocardiaceae and Pseudonocardiaceae in the honeycomb, whereas only Streptomyces genera were isolated by the culture-dependent method. Culture-independent analyses showed more diverse actinobacterial communities than those of culture-dependent methods. The antibacterial bioassay showed that most crude extracts of representative isolates exhibited antibacterial activities. Among them, the crude extract of Streptomyces sp. FCF01 showed the best antibacterial activities against Staphylococcus aureus, Micrococcus tetragenus, and Pseudomonas syringae pv. actinidiae (Psa) with the disc diameter of inhibition zone diameter (IZD) of 23.00, 15.00, and 13.33 mm, respectively. Chemical analysis of Streptomyces sp. FCF01 led to the isolation of three secondary metabolites, including mayamycin (1), mayamycin B (2), and N-(2-Hydroxyphenyl) acetamide (3). Among them, compound 1 displayed strong antibacterial activity against S. aureus, M. tetragenus, and Psa with minimum inhibitory concentrations (MIC) values of 6.25, 12.5, and 6.25 μg/ml, respectively. In addition, two novel derivative compounds 1a and 1b were synthesized by acetylation of compound 1. Both compounds 1a and 1b displayed similar antibacterial activities with those of metabolite 1. These results indicated that Streptomyces species associated with honeybees had great potential in finding antibiotics.

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Community composition, bacterial symbionts, antibacterial and antioxidant activities of honeybee-associated fungi

Cited by 12 publications

References 69 publications

Environment or genetic isolation? An atypical intestinal microbiota in the Maltese honey bee Apis mellifera spp. ruttneri

Environment or genetic isolation? An atypical intestinal microbiota in the Maltese honey bee Apis mellifera spp. ruttneri

Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris

Diversity and antibacterial potential of the Actinobacteria associated with Apis mellifera ligustica

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