Community dynamics of prokaryotic and eukaryotic microbes in an estuary reservoir

Abstract

This study demonstrates both prokaryotic and eukaryotic community structures and dominant taxonomies in different positions of the greatest estuary reservoir for drinking water source in the world in four seasons of one year using 454 pyrosequencing method with total of 312,949 16S rRNA and 374,752 18S rRNA gene fragments, including 1,652 bacteria OTUs and 1,182 fungus OTUs. During winter and spring, the community composition at the phylum level showed that microorganisms had similar structures but their quantities were different. Similarly, obvious changes at the genus level were observed among the samples taken in winter and spring between summer and fall. Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level. Air temperature had a stronger effect than sampling location on the microbial community structure. Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year. Moreover, Bacillus was an efficient indicator during summer and autumn for bacteria OTUs.

Effects of Seasonal Thermal Stratification on the Functional Diversity and Composition of the Microbial Community in a Drinking Water Reservoir

“…Briefly, the V1-V3 region of the 16S rRNA gene of the DNA samples with replicates was PCR amplified using the forward primer 8 F (5′-AGAGTTTGATCCTGGCTCAG-3′) and the reverse primer 553 R (5′-TTACCGCGGCTGCTG GCAC-3′) [19,33]. Polymerase chain reaction was conducted in 20 μL reaction mixtures containing 2.0 μL of deoxynucleoside triphosphate (2.5 mM), 0.8 μL of each forward and reverse primers (5 μM each), 0.4 μL of FastPfu polymerase, 5× FastPfu Buffer 4 μL, and 10 ng of DNA.…”

“…The beta diversity analysis was carried out and Bray-Curtis similarity tree was constructed based on the beta diversity using QIIME [40,41]. Bar plots were done by Microsoft Excel 2010, and heatmap analysis was conducted using R package vegan (version 2.1.1) based on relative abundances of the top 100 abundant bacterial community at genus level [19,42].…”

“…However, the microbial communities in stratified freshwater ecosystems have not been studied to the same extent as those in marine or saline lakes, with different trophic status and morphology. Limited previous research has suggested that each thermal layer in the stratified lakes and reservoirs harbors different bacterial communities [5,18,19]. In addition, other studies based on functional genes, such as the adenosine 5'-phosphosulfate reductase (aprA) and the ammonia monooxygenase A (amoA) genes, showed the strong vertical differentiation of sulfate reducers, sulfur oxidizers, and archaeal ammonia oxidizers along environmental gradients [20,21].…”

Characteristics of Bacterial Communities in Cyanobacteria-Blooming Aquaculture Wastewater Influenced by the Phytoremediation with Water Hyacinth

“…Therefore, it is necessary to pay attention to the characteristics of bacterial communities and the situation of potential pathogens in M. aeruginosa-blooming aquaculture wastewater remediated by water hyacinth. With the rapid development of molecular biological technology, high-throughput sequencing technology has been used in the study of bacterial structure of rivers, lakes, and reservoirs [12][13][14]. We applied culture enumeration and high-throughput sequencing to quantify the changes in the characteristics of bacterial communities, specific bacteria including coliform bacteria, and pathogenic bacteria potentially conducive to human disease by treating cyanobacteria-blooming aquaculture wastewater with water hyacinth.…”

The Effects of Antibiotics on Microbial Community Composition in an Estuary Reservoir during Spring and Summer Seasons

“…To better understand the spatial (sites) and temporal (months) variations of antibiotic concentrations, as well as their co-occurrence pattern with and impact on bacteria and cyanobacteria in surface water of this estuary reservoir during the warm season (from spring to summer) [20,21], we collected water samples from April to September 2014 at a depth of 0.5 m below the surface. All water samples were collected in triplicate, stored in sterile polypropylene bottles and processed immediately upon arrival at the laboratory.…”