Community of dark-spored myxomycetes in ground litter and soil of taiga forest (Nizhne-Svirskiy Reserve, Russia) revealed by DNA metabarcoding

Shchepin, Oleg N.; Schnittler, Martin; Erastova, Daria A.; Prikhodko, Ilya S.; Dahl, Mathilde Borg; Azarov, Daniil; Chernyaeva, Ekaterina; Novozhilov, Yu. K.

doi:10.1016/j.funeco.2018.11.006

Cited by 30 publications

(8 citation statements)

References 52 publications

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“…The study we performed is a record for Ukraine for the amount of collected material (Romanenko, 2002;Leontyev, 2006a), thus allowing us to confirm the patterns earlier found in other regions of the world and detect some earlier undetermined peculiarities of the substrate ecology of myxomycetes. Similarly to the previous studies (Borg Dahl et al, 2019;Shchepin et al, 2019), abundance and diversity of corticulous myxomy-cetes were found to be the highest on the commonest species of substrateforming plants. However, we have shown that differences between the consortia in abundance of myxomycetes cannot be explained only by the number of collected samples.…”

Section: Discussionsupporting

confidence: 81%

Ecological assemblages of corticulous myxomycetes in forest communities of the North-East Ukraine

Kochergina

Markina

2021

Biosys. divers.

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Corticulous myxomycetes remain one of the least surveyed ecological groups of terrestrial protists. These organisms develop on the bark of trees, mostly feeding on bacteria and microalgae. Their microscopic size and fast developmental cycle (3–5 days) complicate the study of these organisms, and therefore data their on ecological relationships and patterns of biodiversity corticulous myxomycetes remain controversial. On the territory of the southwest spurs of the Central Russian Upland (Northeast Ukraine), no special studies on these organisms have been conducted. During 2017–2020, in nine forest sites located in this territory, we collected samples of bark of 16 species of tree plants, on which sporulating myxomycetes were then identified using the moist chamber technique in laboratory conditions. A total of 434 moist chambers was prepared, and 267 (61.5%) of which were found to contain myxomycete fruiting bodies. In total, we made 535 observations, finding 20,211 sporocarps. As a result, in the surveyed territory, we found 38 species of corticulous myxomycetes, belonging to 18 genera, 10 families, 7 orders, and 2 subclasses of Myxomycetes. Among the species of corticulous myxomycetes, the most abundant were Echinostelium minutum, Arcyria pomiformis, Macbrideola cornea, Perichaena chrysosperma, Licea kleistobolus, Paradiacheopsis fimbriata, Cribraria violacea, Enerthenema papillatum, A. cinerea, and L. operculata. The greatest species richness in the examined biota was observed for genera Comatricha, Licea, Paradiacheopsis and Perichaena, families Amaurochaetaceae and Trichiaceae, orders Stemonitidales, Trichiales and Physarales. By species diversity, dark-spored myxomycetes (Collumellomycetidae) somewhat exceeded bright-spored myxomycetes (Lucisporomycetidae). Badhamia versicolor, Didymium dubium, D. sturgisii, Macbrideola decapillata, and Perichaena luteola are new species for the surveyed area. Four species of myxomycetes were collected in Ukraine for the first time: Hemitrichia pardina, Licea floriformis, L. pygmea, and Macbrideola argentea. Quantitative and qualitative structure of myxomycete consortia developing on different species of substrate-forming plants demonstrated significant differences. The highest level of similarity was demonstrated by Fraxinus excelsior and Acer platanoides, and a relatively strong relationship was seen between Pinus sylvestris and Tilia cordata. The central cluster comprised F. excelsior, A. platanoides and P. sylvestris. By the sum of values of Bray-Curtis coefficient, Quercus robur appeared to be most distinctive plant species by quantitative composition of myxomycete consortia. F. excelsior and T. cordata are the most favourable for the development of corticulous myxomycetes. In all the analyzed consortia, the dominant species belonged to the Stemonitidales and Trichiales orders, while the remaining orders were represented by notably fewer species. Relative species richness of Stemonitidales was the highest in consortia of P. sylvestris, the contribution of Liceales was the greatest in A. platanoides and P. sylvestris, the percentage of Echinosteliales and Physarales was the highest on F. excelsior, the share of Cribrariales was especially large on A. platanoides. Trichiales were represented on all the analyzed substrates to almost the same extent. Representatives of Cribrariales and Physarales were completely absent on P. sylvestris, the species of Clastodermatales – on all species of plants, except Q. robur. Prevalence of bright-spored myxomycetes was determined for consortia of Acer platanoides, the dominance of dark-spored myxomycetes – for F. excelsior, P. sylvestris and Q. robur. The obtained data indicate the presence of stable complexes of corticulous myxomycetes, associated with different species of trees in the forest ecosystems of Northeast Ukraine. This encourages further study of the structure of myxomycete consortia with tree species that were not included in this study and determining the influence of physical-chemical properties of the bark of different plant species on the discovered peculiarities of myxomycete communities.

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Section: Discussionsupporting

confidence: 81%

Ecological assemblages of corticulous myxomycetes in forest communities of the North-East Ukraine

Kochergina

Markina

2021

Biosys. divers.

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“…Previously one sequence of this species (LE306548) was submitted to GenBank under No. MH930789 but with the incorrect determination Stemonitis laxifila (Shchepin et al 2019). A phylogenetic analysis of the 18S rDNA sequences (SSU) revealed 100% similarity with the studied specimen of S. capillitionodosa (LE306833, GenBank No.…”

Section: Annotated Species Listmentioning

confidence: 99%

Myxomycetes of boreal forests of the Laplandskiy State Nature Biosphere Reserve (Kola Peninsula, Russia) Yuri

Novozhilov¹,

Shchepin²,

Gmoshinskiy³

et al. 2020

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Northern taiga forests and subalpine plant communities of the Laplandskiy State Nature Biosphere Reserve (Kola Peninsula, Russia) were surveyed for myxomycetes. A total of 1675 specimens of myxomycete fruit bodies (sporocarps) were registered, among them 1584 records from field collections and 92 obtained from 210 moist chamber cultures of ground litter, bark of living trees, wood, and weathered dung of moose and willow ptarmigan. Most of 125 taxa (124 morphospecies and one variety) representing 34 genera were recorded only in the field (104 taxa from 32 genera), but some were exclusively obtained from moist chamber cultures (8 taxa from 5 genera). All of the recorded species are new for the Laplandskiy Reserve. Species numbers decreased among the four studied forest associations along the elevation and mositure gradient, and the Shannon index showed a similar trend: spruce forest (PICa; 84 taxa, H’=3.8), spruce-peat moss forest (PICb; 70, 3.5), dry spruce-pine forest (PIN; 62, 3.7), subalpine birch forest (SB; 30, 2.7). The estimated completeness of the survey according to the Chao1 estimator was 66%, indicating that most of the more common species should have been recovered. The trend among forest associations runs mainly parallel to diversity: PICa and PICb 83%, PIN 47%, SB 57%. The myxomycete assemblage of dry coniferous forests is the most distinctive among the three forest types and shows the highest number of indicator species. The overall degree of specialization of myxomycetes is higher for substrate type than for forest associations. Among substrate types, species diversity and richness increase from litter over bark to wood.

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“…To evaluate the taxonomical resolution of SSU-based DNA barcoding at the intramorphospecies level, SSU sequences of Ph. albescens were clustered with two similarity thresholds that were applied for species determination or clustering of the operational taxonomic units (OTUs) in previous studies: (1) 99.1% (Borg Dahl et al, 2019;Borg Dahl, Brejnrod, et al, 2018;Borg Dahl, Shchepin, et al, 2018;Shchepin et al, 2017Shchepin et al, , 2019 and ( 2) 98% (Clissmann et al, 2015;Gao et al, 2019;Kamono et al, 2013;Shchepin et al, 2019). SSU sequences were trimmed to match ca.…”

Section: Sequence Clusteringmentioning

confidence: 99%

“…However, since other mountains in North America are sampled poorly or not sampled at all, the real centre of diversity can be somewhere else on this continent. Borg Dahl, Brejnrod, et al, 2018;Borg Dahl, Shchepin, et al, 2018;Shchepin et al, 2017Shchepin et al, , 2019, (2) 98% (Clissmann et al, 2015;Gao et al, 2019;Kamono et al, 2013;Shchepin et al, 2019),…”

Section: Geographical Distributionmentioning

confidence: 99%

Genetic structure of the protist Physarum albescens (Amoebozoa) revealed by multiple markers and genotyping by sequencing

et al. 2021

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Community of dark-spored myxomycetes in ground litter and soil of taiga forest (Nizhne-Svirskiy Reserve, Russia) revealed by DNA metabarcoding

Cited by 30 publications

References 52 publications

Ecological assemblages of corticulous myxomycetes in forest communities of the North-East Ukraine

Ecological assemblages of corticulous myxomycetes in forest communities of the North-East Ukraine

Myxomycetes of boreal forests of the Laplandskiy State Nature Biosphere Reserve (Kola Peninsula, Russia) Yuri

Genetic structure of the protist Physarum albescens (Amoebozoa) revealed by multiple markers and genotyping by sequencing

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