Community structure and dynamics of the free and attached microorganisms during moderately thermophilic bioleaching of chalcopyrite concentrate

Zeng, Weimin; Qiu, Guanzhou; Zhou, Hongbo; Peng, Jun; Chen, Miao; Tan, Su Nee; Chao, Wei-Liang; Liu, Xueduan; Zhang, Yansheng

doi:10.1016/j.biortech.2010.04.003

Cited by 101 publications

(35 citation statements)

References 35 publications

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“…The redox potential was measured with a platinum electrode with an Ag/AgCl electrode as reference. Attached microorganisms were detached from the minerals by vigorous vortex vibrate with glass beads, the details according to the study of Zeng et al [24]. The total amounts of attached and free cells were analyzed by observation with a blood cell counting chamber under an optical microscope.…”

Section: Bioleaching Experimentsmentioning

confidence: 99%

Expression of Critical Sulfur- and Iron-Oxidation Genes and the Community Dynamics During Bioleaching of Chalcopyrite Concentrate by Moderate Thermophiles

et al. 2015

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Sulfate adenylyltransferase gene and 4Fe-4S ferredoxin gene are the key genes related to sulfur and iron oxidations during bioleaching system, respectively. In order to better understand the bioleaching and microorganism synergistic mechanism in chalcopyrite bioleaching by mixed culture of moderate thermophiles, expressions of the two energy metabolism genes and community dynamics of free and attached microorganisms were investigated. Specific primers were designed for real-time quantitative PCR to study the expression of these genes. Real-time PCR results showed that sulfate adenylyltransferase gene was more highly expressed in Sulfobacillus thermosulfidooxidans than that in Acidithiobacillus caldus, and expression of 4Fe-4S ferredoxin gene was higher in Ferroplasma thermophilum than that in S. thermosulfidooxidans and Leptospirillum ferriphilum. The results indicated that in the bioleaching system of chalcopyrite concentrate, sulfur and iron oxidations were mainly performed by S. thermosulfidooxidans and F. thermophilum, respectively. The community dynamics results revealed that S. thermosulfidooxidans took up the largest proportion during the whole period, followed by F. thermophilum, A. caldus, and L. ferriphilum. The CCA analysis showed that 4Fe-4S ferredoxin gene expression was mainly affected (positively correlated) by high pH and elevated concentration of ferrous ion, while no factor was observed to prominently influence the expression of sulfate adenylyltransferase gene.

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Section: Bioleaching Experimentsmentioning

confidence: 99%

Expression of Critical Sulfur- and Iron-Oxidation Genes and the Community Dynamics During Bioleaching of Chalcopyrite Concentrate by Moderate Thermophiles

et al. 2015

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“…It is well documented that the optimum pH for S. thermosulfidooxidans' growth is 1.6 (Jingshi and Renman, 2002) and the pH value 1.5-2.0 is suitable for the growth of A. caldus (Plumb et al, 2008), so A. caldus was predominant at the early stage, while the growths of S. thermosulfidooxidans and A. caldus were both inhibited with the decrease of pH at later bioleaching stage. Sand et al found that ferrous iron was released on the mineral surface as the dissolution of pyrite (Sand and Gehrke, 2006), which can promote the growth of attached iron oxidizer L. ferriphilum (Zeng et al, 2010). So S. thermosulfidooxidans also as an iron oxidizer, whose growth was supposed to be promoted as well, became predominant relatively at the later stage.…”

Section: Dgge Analysismentioning

confidence: 96%

Microbial community changes during the process of pyrite bioleaching

Yin

Wang

et al. 2012

Hydrometallurgy

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“…The negative controls were also designed. Procedures of PCR and qPCR were as described by previous research studies (Li et al 2011;Wang et al 2012;Zeng et al 2010;Zhang et al 2009). All tests were conducted in triplicate.…”

Section: Stirred Tank Reactor Bioleaching Experimentsmentioning

confidence: 99%

“…To our knowledge, there have been no reports yet about community dynamics on multiple heavy metal sediment bioleaching. Microbial ecological studies estimate the interaction of microbial groups, which groups can be correlated to chemical and physical variation during bioleaching (Johnson 2001;Johnson 1998;Zeng et al 2010). Understanding community structure and dynamic is of importance for optimizing the leaching parameters (He et al 2012;Xia et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Bioleaching of multiple heavy metals from contaminated sediment by mesophile consortium

Gan

Zhou

et al. 2014

Environ Sci Pollut Res

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A defined mesophile consortium including Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirilum ferriphilum was applied in bioleaching sediments contaminated with multiple heavy metals. Flask experiments showed that sulfur favored the acidification in the early stage while pyrite led to a great acidification potential in the later stage. An equal sulfur/pyrite ratio got the best acidification effect. Substrate utilization started with sulfur in the early stage, and then the pH decline and the community shift give rise to the utilization of pyrite. Solubilization efficiency of Zn, Cu, Mn, and Cd reached 96.1, 93.3, 92.13, and 87.65%, respectively. Bioleaching efficiency of other elements (As, Hg, Pb) was not more than 30%. Heavy metal solubilization was highly negatively correlated with pH variation. Logistic models were well fitted with the solubilization efficiency, which can be used to predict the bioleaching process. The dominant species in the early stage of bioleaching were A. ferrooxidans and A. thiooxidans, and the abundance of L. ferriphilum increased together with pyrite utilization and pH decline.

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Community structure and dynamics of the free and attached microorganisms during moderately thermophilic bioleaching of chalcopyrite concentrate

Cited by 101 publications

References 35 publications

Expression of Critical Sulfur- and Iron-Oxidation Genes and the Community Dynamics During Bioleaching of Chalcopyrite Concentrate by Moderate Thermophiles

Expression of Critical Sulfur- and Iron-Oxidation Genes and the Community Dynamics During Bioleaching of Chalcopyrite Concentrate by Moderate Thermophiles

Microbial community changes during the process of pyrite bioleaching

Bioleaching of multiple heavy metals from contaminated sediment by mesophile consortium

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