Community structure of aerobic anoxygenic phototrophic bacteria in algae- and macrophyte-dominated areas in Taihu Lake, China

Shi, Limei; Cai, Yuanfeng; Shi, Xiaoli; Zhang, Min; Zeng, Qingfei; Kong, Fanxiang; Xu, Ping

doi:10.1007/s00343-022-1348-2

Cited by 6 publications

(5 citation statements)

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“…AAP bacteria peak during spring in lakes, when they may account for up to 22% of bacteria [18,19]. Their abundances and diversity dynamics correlate with irradiance, temperature, chlorophyll-a (Chl-a), oxygen and DOC [18,[20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

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Phenology and ecological role of Aerobic Anoxygenic Phototrophs in fresh waters

Villena-Alemany,

Mujakić,

Fecskeová

et al. 2023

Preprint

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Aerobic anoxygenic phototrophic (AAP) bacteria are heterotrophic bacteria that supply their metabolism with light energy harvested by bacteriochlorophyll-a-containing reaction centres. Despite their substantial contribution to bacterial biomass, microbial food webs and carbon cycle, their phenology in freshwater lakes remains unknown. Hence, we investigated seasonal variations of AAP abundance and community composition biweekly across three years in a temperate, meso-oligotrophic freshwater lake. AAP bacteria displayed a clear seasonal trend with a spring maximum following the bloom of phytoplankton and a secondary maximum in autumn. As the AAP bacteria represent a highly diverse assemblage of species, we followed their seasonal succession using the amplicon sequencing of thepufM marker gene. To enhance the accuracy of the taxonomic assignment, we developed newpufM primers that generate longer amplicons and compiled the currently largest database ofpufM gene, comprising 3633 reference sequences spanning all phyla known to contain AAP species. With this novel resource we demonstrated recurrent and dynamic seasonal succession of the AAP community. The majority of the species appeared during specific phases of the seasonal cycle, with less than 2% of AAP species detected during the whole year. Our results document the indigenous freshwater nature of the AAP community, characterized by high resilience and heterogenic adaptations to varying conditions of the freshwater environment. By integrating this information with the indicator of primary production (Chlorophyll-a) and existing ecological models, we show that AAP bacteria play a pivotal role in the recycling of dissolved organic matter released during spring phytoplankton bloom, contributing significantly to the ecological dynamics of lakes.

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Section: Introductionmentioning

confidence: 99%

“…The copyright holder for this preprint this version posted November 17, 2023. ; https://doi.org/10.1101/2023.11.17.567504 doi: bioRxiv preprint abundances and diversity dynamics correlate with irradiance, temperature, chlorophyll-a (Chl-a), oxygen and DOC [18,[20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Phenology and ecological role of Aerobic Anoxygenic Phototrophs in fresh waters

Villena-Alemany,

Mujakić,

Fecskeová

et al. 2023

Preprint

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“…was significantly lower in summer, while MAG147 ( Rhizobiales bacterium) was significantly higher in colder months. A previous study also observed that the Rhizobiales were especially enriched in cold seasons ( Shi et al, 2022b ) indicating they might be psychrophilic bacteria. In addition, dinitrogen can be converted to ammonia by nitrogen fixation microbes in activated sludge ( Rose et al, 2021 ), while we found 26 MAGs were diazotrophs with anfG or nifDHK , in which eight were archaea.…”

Section: Discussionmentioning

confidence: 71%

Monthly dynamics of microbial communities and variation of nitrogen-cycling genes in an industrial-scale expanded granular sludge bed reactor

Zhang

Zhang²,

Deng³

et al. 2023

Front. Microbiol.

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IntroductionThe expanded granular sludge bed (EGSB) is a major form of anaerobic digestion system during wastewater treatment. Yet, the dynamics of microbial and viral communities and members functioning in nitrogen cycling along with monthly changing physicochemical properties have not been well elucidated.MethodsHere, by collecting the anaerobic activated sludge samples from a continuously operating industrial-scale EGSB reactor, we conducted 16S rRNA gene amplicon sequencing and metagenome sequencing to reveal the microbial community structure and variation with the ever-changing physicochemical properties along within a year.ResultsWe observed a clear monthly variation of microbial community structures, while COD, the ratio of volatile suspended solids (VSS) to total suspended solids (TSS) (VSS/TSS ratio), and temperature were predominant factors in shaping community dissimilarities examined by generalized boosted regression modeling (GBM) analysis. Meanwhile, a significant correlation was found between the changing physicochemical properties and microbial communities (p <0.05). The alpha diversity (Chao1 and Shannon) was significantly higher (p <0.05) in both winter (December, January, and February) and autumn (September, October, and November) with higher organic loading rate (OLR), higher VSS/TSS ratio, and lower temperature, resulting higher biogas production and nutrition removal efficiency. Further, 18 key genes covering nitrate reduction, denitrification, nitrification, and nitrogen fixation pathways were discovered, the total abundance of which was significantly associated with the changing environmental factors (p <0.05). Among these pathways, the dissimilatory nitrate reduction to ammonia (DNRA) and denitrification had the higher abundance contributed by the top highly abundant genes narGH, nrfABCDH, and hcp. The COD, OLR, and temperature were primary factors in affecting DNRA and denitrification by GBM evaluation. Moreover, by metagenome binning, we found the DNRA populations mainly belonged to Proteobacteria, Planctomycetota, and Nitrospirae, while the denitrifying bacteria with complete denitrification performance were all Proteobacteria. Besides, we detected 3,360 non-redundant viral sequences with great novelty, in which Siphoviridae, Podoviridae, and Myoviridae were dominant viral families. Interestingly, viral communities likewise depicted clear monthly variation and had significant associations with the recovered populations (p <0.05).DiscussionOur work highlights the monthly variation of microbial and viral communities during the continuous operation of EGSB affected by the predominant changing COD, OLR, and temperature, while DNRA and denitrification pathways dominated in this anaerobic system. The results also provide a theoretical basis for the optimization of the engineered system.

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“…AAP bacteria peak during spring in lakes, when they may account for up to 22% of bacteria [ 18 , 19 ]. Their abundances and diversity dynamics correlate with irradiance, temperature, chlorophyll- a (Chl- a ), oxygen, and DOC [ 18 , 20 – 24 ].…”

Section: Introductionmentioning

confidence: 99%

Phenology and ecological role of aerobic anoxygenic phototrophs in freshwaters

Villena-Alemany,

Mujakić,

Fecskeová

et al. 2024

Microbiome

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Background Aerobic anoxygenic phototrophic (AAP) bacteria are heterotrophic bacteria that supply their metabolism with light energy harvested by bacteriochlorophyll-a-containing reaction centers. Despite their substantial contribution to bacterial biomass, microbial food webs, and carbon cycle, their phenology in freshwater lakes remains unknown. Hence, we investigated seasonal variations of AAP abundance and community composition biweekly across 3 years in a temperate, meso-oligotrophic freshwater lake. Results AAP bacteria displayed a clear seasonal trend with a spring maximum following the bloom of phytoplankton and a secondary maximum in autumn. As the AAP bacteria represent a highly diverse assemblage of species, we followed their seasonal succession using the amplicon sequencing of the pufM marker gene. To enhance the accuracy of the taxonomic assignment, we developed new pufM primers that generate longer amplicons and compiled the currently largest database of pufM genes, comprising 3633 reference sequences spanning all phyla known to contain AAP species. With this novel resource, we demonstrated that the majority of the species appeared during specific phases of the seasonal cycle, with less than 2% of AAP species detected during the whole year. AAP community presented an indigenous freshwater nature characterized by high resilience and heterogenic adaptations to varying conditions of the freshwater environment. Conclusions Our findings highlight the substantial contribution of AAP bacteria to the carbon flow and ecological dynamics of lakes and unveil a recurrent and dynamic seasonal succession of the AAP community. By integrating this information with the indicator of primary production (Chlorophyll-a) and existing ecological models, we show that AAP bacteria play a pivotal role in the recycling of dissolved organic matter released during spring phytoplankton bloom. We suggest a potential role of AAP bacteria within the context of the PEG model and their consideration in further ecological models.

show abstract

Community structure of aerobic anoxygenic phototrophic bacteria in algae- and macrophyte-dominated areas in Taihu Lake, China

Cited by 6 publications

References 70 publications

Phenology and ecological role of Aerobic Anoxygenic Phototrophs in fresh waters

Phenology and ecological role of Aerobic Anoxygenic Phototrophs in fresh waters

Monthly dynamics of microbial communities and variation of nitrogen-cycling genes in an industrial-scale expanded granular sludge bed reactor

Phenology and ecological role of aerobic anoxygenic phototrophs in freshwaters

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