This paper proposes a nature-inspired spider web-shaped ultra-high frequency (UHF) radio frequency identification (RFID) reader antenna and battery-free sensor-based system for healthcare applications. This antenna design consists of eight concentric decagons of different sizes and five straight microstrip lines that are connected to the ground using 50 Ω resistors from both ends except one microstrip line, which is left for connecting a feeding port. The reader antenna design features fairly strong and uniform electric and magnetic field characteristics. Likewise, it also poses wideband characteristics by covering whole UHF RFID band (860 – 960 MHz) and tag reading volume of 200 × 200 × 20 mm 3. Additionally, it has low gain characteristics, which are necessary for the majority of nearfield applications to prevent the misreading of other tags. Moreover, the symmetric current distribution throughout the structure in this design addresses the orientation sensitivity issues associated with low-cost linearly polarized tag antennas. The measurement results show that the reader antenna can read medicine pills tagged using low-cost passive/battery-free RFID tags, tagged expensive jewelry, intervenes solution, and blood bags positioned in various orientations. As a result, the proposed reader antenna-based system is a strong contender for near-field RFID, healthcare, and IoT applications.