Compact low‐cost high‐voltage pulse generator for biological applications

Achour, Yahia; Starzyński, Jacek; Kasprzycka, Wiktoria; Trafny, Elżbieta Anna

doi:10.1002/cta.2708

Cited by 10 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This approach reduced the size and cost of the generator and increased its performance and the controllability of the generated pulses. A full description of the architecture and operation of the pulse generator has been described previously [ 85 ]. The generated pulses had a quasi-rectangular shape, a 60 ns pulse width, and 5.5 kV amplitude, which corresponded to an electric field within the cuvette of 14 kV/cm.…”

Section: Methodsmentioning

confidence: 99%

Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

Kasprzycka

Trębińska-Stryjewska

Lewandowski

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to verify whether the nanosecond pulsed electric field, not eliciting thermal effects, permanently changes the molecular processes and gene expression of Leydig TM3 cells. The cells were exposed to a moderate electric field (80 quasi-rectangular shape pulses, 60 ns pulse width, and an electric field of 14 kV/cm). The putative disturbances were recorded over 24 h. After exposure to the nanosecond pulsed electric field, a 19% increase in cell diameter, a loss of microvilli, and a 70% reduction in cell adhesion were observed. Some cells showed the nonapoptotic externalization of phosphatidylserine through the pores in the plasma membrane. The cell proportion in the subG1 phase increased by 8% at the expense of the S and G2/M phases, and the DNA was fragmented in a small proportion of the cells. The membrane mitochondrial potential and superoxide content decreased by 37% and 23%, respectively. Microarray’s transcriptome analysis demonstrated a negative transient effect on the expression of genes involved in oxidative phosphorylation, DNA repair, cell proliferation, and the overexpression of plasma membrane proteins. We conclude that nanosecond pulsed electric field affected the physiology and gene expression of TM3 cells transiently, with a noticeable heterogeneity of cellular responses.

show abstract

Section: Methodsmentioning

confidence: 99%

Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

Kasprzycka

Trębińska-Stryjewska

Lewandowski

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…For this reason, many studies were carried out to develop robust, efficient, flexible, and easy-to-use generators. Many of them are based on the classical architecture of a Marx generator (shown in Figure 1), such as in [13][14][15][16][17], transmission lines and Blumleinbased generators [18,19], RLC pulse-forming networks [20], and pulse transformer-based generators [21]. Besides biomedical treatments, pulsed power technologies have many other applications, such as plasma science [22][23][24], industrial process [25], ultrawideband radiation, and EMP generation [26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

New Architecture of Solid-State High-Voltage Pulse Generators

2022

Self Cite

View full text Add to dashboard Cite

The application of the nanosecond pulsed electric field (nsPEF) for biomedical treatments has gained more interest in recent decades due to the development of pulsed power technologies which provides the ability to control the electric field dose applied during tests. In this context, the proposed paper describes a new architecture of solid-state high-voltage pulse generators (SS-HVPG) designed to generate fully customised sequences of quasi-rectangular pulses. The idea is based on the combination of semiconductor switches (IGBT/MOSFET) known for their flexibility and controllability with special magnetic switches to build compact and modular generators. The proposed structure is inspired by the most known pulse generator of Marx, but mixes its two variants for negative and positive polarities. Thus, the polarity of the generated pulses can be freely selected. In addition to that, the use of IGBTs/MOSFET ensures a tunable repetition rate and pulse width. The capacitors are charged via a series of magnetic switches and a flyback DC–DC converter which provides fast and efficient charging and also an adjustable amplitude of the output pulses. The design can be easily simplified giving two other modified structures, based on the same idea, for mono-polar operating (only positive or only negative pulses) with a reduced number of switches. A SPICE simulation of the generator and results of experimental tests carried out on a three stages generator are presented. The obtained results confirm the operating principle and the claimed performances of the new structure.

show abstract

“…The generation of high-voltage pulses is needed for carrying out a variety of tests and research. These concern not only the simulation of lightning discharges [1] or dielectric strength tests, but also the acceleration of particles [2][3][4][5], the generation of strong X-rays [6], high-power microwaves [7], biological applications [8] or food processing [9,10]. Marx generators are very often used in such applications.…”

Section: Introductionmentioning

confidence: 99%

High-Voltage Power Supply for High Repetitive Rate Marx Generator with Quasi-Resonant Zero-Current Switching Transistor Control Algorithm

Pachowicz

2022

Energies

View full text Add to dashboard Cite

Due to having a number of advantages, Marx generators are still the most widely used devices for generating high-voltage pulses in many fields of science and technology. To ensure their proper operation, especially when the generation of many frequent, highly repetitive pulses is required, a highly efficient high-voltage power supply is needed. The paper describes a specially developed power supply (input voltage 48 V DC, output voltage up to 50 kV) based on the conventional Full Bridge topology with two high-frequency high-voltage transformers and a 6-stage voltage multiplier. In order to avoid many problems caused by low coupling between primary and secondary windings of the transformers and the large parasitic capacitances of the secondary windings, a special quasi-resonant zero-current switching transistor control algorithm with variable switching frequency (dependent on output load) was developed. In the described method, the energy is supplied to the transformer in short pulses, when a pair of diagonal transistors of the full-bridge converter were turned on. Then, the freewheeling state is maintained until all of the energy stored in the leakage inductance of the transformer has been transferred to the secondary side, which means that the current in the primary windings drops to zero. This approach reduces energy losses, electromagnetic disturbances and prevents current distortion in primary winding.

show abstract

Compact low‐cost high‐voltage pulse generator for biological applications

Cited by 10 publications

References 29 publications

Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

New Architecture of Solid-State High-Voltage Pulse Generators

High-Voltage Power Supply for High Repetitive Rate Marx Generator with Quasi-Resonant Zero-Current Switching Transistor Control Algorithm

Contact Info

Product

Resources

About