Compaction and decompaction of DNA dominated by the competition between counterions and DNA associating with cationic aggregates

Xu, Lu; Feng, Lei; Hao, Jingcheng; Dong, Shuli

doi:10.1016/j.colsurfb.2015.06.038

Cited by 39 publications

(50 citation statements)

References 39 publications

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“…The interactions of cationic compounds with DNA result in the formation of biochemical constructs that are of significant importance in gene diagnostic and therapy [1][2][3][4]. Cationic surfactants are in the focus of modern biotechnological applications as relatively simple and effective carriers of therapeutic agents that assist in transfer of genetic material.…”

Section: Introductionmentioning

confidence: 99%

Structural, biocomplexation and gene delivery properties of hydroxyethylated gemini surfactants with varied spacer length

Zakharova

Gabdrakhmanov

Ibragimova

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Section: Introductionmentioning

confidence: 99%

Structural, biocomplexation and gene delivery properties of hydroxyethylated gemini surfactants with varied spacer length

Zakharova

Gabdrakhmanov

Ibragimova

et al. 2016

Colloids and Surfaces B: Biointerfaces

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“…The significant reduction in charge repulsion between positively charged CQDCe through electrostatic interactions decreases the thermodynamic stability of the CQDCe–DNA complexes and causes their aggregation and association. This phenomenon is analogous to DNA–surfactant self‐assembly, in which the DNA charge is neutralized close to 0 mV, accompanied by precipitation in solution . At the csc, the CQDCe–DNA complexes possess a large average size of 1015 nm and high polydispersity index (PDI) of 0.473 (Figure d), which results in an increase in the optical density (OD) to 0.094±0.0036 (Figure c).…”

Section: Resultsmentioning

confidence: 83%

“…Thus, the CD signals clearly show a redshift of both the positive and negative bands. As previously reported, the observed redshift to longer wavelengths for CD peaks of DNA upon complexation with cationic surfactants has been attributed to a structure transition from the B‐ to the C‐form, accompanied by a DNA compaction process . In addition, some studies have insisted that the changes in the CD signals are due to minor changes in the interactions between the bases and that the double helix maintains the B‐form.…”

Section: Resultsmentioning

confidence: 99%

“…[21] As expected, when positive-charged CQDCe interacts with DNA, the value of x for the CQDCe-DNA complexes gradually decreases from 45.99 AE 0.86 mV (no DNA addition) to À17.2 AE 0.31 mV (with 300 mmol mL À1 DNA;F igure 2b). [22] At the csc, the CQDCe-DNA complexes possess al arge average size of 1015 nm andh igh polydispersity index (PDI) of 0.473 (Figure 2d), which results in an increasei n the optical density (OD) to 0.094 AE 0.0036 ( Figure 2c). At the csc, the surface charges of CQDCe are completely neutralized by DNA molecules and x = 0mV( Figure 2b).…”

Section: Self-assembly Behavior and Cqdce-dna Vesicle Formationmentioning

confidence: 99%

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Versatile Self‐Assembly and Biosensing Applications of DNA and Carbon Quantum Dots Coordinated Cerium Ions

Wang

Sun

Zhang

et al. 2017

Chemistry A European J

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Self-assembly exploits noncovalent interactions to offer a facile and effective method for the construction of soft materials with multifunctionalities and diversity. In this work, fluorescence carbon quantum dots coordinated by Ce ions (CQDCe) have been synthesized and exploited as building blocks to generate a series of hierarchical structures through the ionic self-assembly of CQDCe and biomolecules, namely DNA, myoglobin (Mb), and hyaluronic acid (HA). In particular, vesicles can be constructed by the simple mixing of oppositely charged CQDCe and DNA in water. The formation of unusual vesicles can be explained by the self-assembly of CQDCe with a rearranged structure and the rigid DNA biomolecular scaffolds. This facile noncovalent self-assembly method has inspired the innovative use of virgin DNA as a building block to construct vesicles rather than resorting to a sophisticated synthesis. The self-assembly of CQDCe-biopolymers was accompanied by aggregation-induced photoluminescence (PL) quenching. The biosensing platform was designed to detect polypeptides and deoxyribonuclease I through competitive binding of CQDCe and enzymatic hydrolysis of the DNA backbone, respectively. We believe that the integrative self-assembly of CQDCe and DNA will enrich the theoretical study of vesicle formation by DNA molecules and extend the application of fluorescence carbon quantum dots in the biological field.

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“…Recently, it has been reported that the precipitates formed in the DNA/CTABr (hexadecyltrimethylammonium bromide)complex system can be redissolved by the addition of an excess of cationic CTABr [9][10][11], which had been demonstrated to be impossible in previous studies [12,13]. But there were the controversial conclusions To the best of our knowledge, the controlled compaction and decompaction of DNA by zwitterionic surfactants alone have not yet been reported.…”

Section: Introductionmentioning

confidence: 98%

Controlled compaction and decompaction of DNA by zwitterionic surfactants

Feng

Hao

et al. 2016

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Controlled compaction and decompaction of DNA by zwitterionic surfactants, alkyldimethylamine oxides (C n DMAO, n = 10, 12, and 14), were investigated by various analytical tools. It was found that DNA could effectively be compacted by cationic micelles of C n DMAOH + which were produced by the protonation of C n DMAO in acidic media leading to the formation of water-insoluble C n DMAOH + /DNA complexes. The DNA molecules were compacted at pH 4-5 when the concentration of C 10 DMAOH + , C 12 DMAOH + , and C 14 DMAOH + reached 8.0, 1.6, and 0.9 mmol•L-1 , respectively. Interestingly, the precipitates of C n DMAOH + /DNA complexes can re-dissolve which indicated that the DNA molecules were released from the complexes by regulating the pH of the solution to ~4 and increasing the surfactant concentration to 40, 9.0, and 1.8 mmol•L-1 for C 10 DMAOH + , C 12 DMAOH + , and C 14 DMAOH + , respectively. This phenomenon was attributed to the hydrogen bonding formed between cationic C n DMAOH + and zwitterionic C n DMAO species. These hydrogen-bonded species screen the electrostatic forces between the positively charged C n DMAOH + micelles and the negatively charged backbones of DNA. Our results demonstrated that the release of DNA from the C n DMAOH + /DNA precipitates depended on the concentration of cationic C n DMAOH + and the pH of the solution. Compared with the conventional release of DNA by the addition of β-cyclodextrin, the present strategy allowed for a specific controlled release, which favored the penetration of DNA into cells and could protect the DNA from nucleases degradation.

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Compaction and decompaction of DNA dominated by the competition between counterions and DNA associating with cationic aggregates

Cited by 39 publications

References 39 publications

Structural, biocomplexation and gene delivery properties of hydroxyethylated gemini surfactants with varied spacer length

Structural, biocomplexation and gene delivery properties of hydroxyethylated gemini surfactants with varied spacer length

Versatile Self‐Assembly and Biosensing Applications of DNA and Carbon Quantum Dots Coordinated Cerium Ions

Controlled compaction and decompaction of DNA by zwitterionic surfactants

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