Comparability of different methods of glycated hemoglobin measurement for samples of patients with variant and non-variant hemoglobin

Zhang, Rui; Song, Yichuan; Xu, Anping; Wang, Mo; Ji, Ling; Wang, Qingtao; Shi, Jie; Zhao, Rui; Fu, Wenxaun

doi:10.1016/j.cca.2022.06.024

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…These methods make use of the difference in charge between HbA 1c and HbA 0 or the structural differences between glycated and non-glycated forms of hemoglobin 6 . Each method exhibits distinct characteristics and performance and may be affected by factors such as lipemia, anemia, drugs, or hemoglobin variants 7 – 10 . Therefore, it is necessary to conduct a comprehensive performance evaluation of HbA 1c assays prior to their use in the clinical laboratory.…”

Section: Introductionmentioning

confidence: 99%

Analytical performance evaluation of the Mindray enzymatic assay for hemoglobin A1c measurement

Li,

Wu,

Xie

et al. 2024

Sci Rep

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Hemoglobin A1c (HbA1c) plays a crucial role in diabetes management. We aimed to evaluate the analytical performance of a new enzymatic method kit for HbA1c measurement. The performance of the enzymatic method, including precision, accuracy, and linearity, was evaluated. Moreover, the interference effect from conventional interferents, Hb derivatives, Hb variants, and common drugs were assessed. In addition, the agreement of HbA1c results was compared between enzymatic methods, cation-exchange high-performance liquid chromatography (HPLC), and immunoassays. The intra-assay, between-assay, and total precision of HbA1c were all lower than 2%. HbA1c showed good linearity within the range of 3.96–20.23%. The enzymatic assay yielded results consistent with the external quality control samples, with a bias of less than ± 6% from the target values. The enzymatic method showed no interference from bilirubin, intralipid, vitamin C, Hb derivatives, common Hb variants, as well as antipyretic analgesics and hypoglycemic drugs. The HbA1c results of the enzymatic assay showed good agreement and accuracy compared to those obtained from the HPLC method and the immunoassay. The enzymatic method kit performed on the BS-600M chemistry analyzer is a reliable and robust method for measuring HbA1c. It is suitable for routine practice in clinical chemistry laboratories.

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Section: Introductionmentioning

confidence: 99%

Analytical performance evaluation of the Mindray enzymatic assay for hemoglobin A1c measurement

Li,

Wu,

Xie

et al. 2024

Sci Rep

Self Cite

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Early developments toward HbA1c determination in whole blood by high-speed sample preparation and LC–MS/MS analysis

Mitra,

Leinenbach,

Geistanger

et al. 2024

Anal Bioanal Chem

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We report a method to determine HbA1c (glycated hemoglobin) where whole blood samples are prepared by fast hemolysis (dilution with deionized water and vortex mixing), digestion with 0.6 mg/mL endoproteinase Glu C (Glu C) in 30 mM ammonium acetate buffer (pH 4.3) at 37 °C for 45 min, and termination of the digestion by diluting with 0.1% formic acid in water, and then analysis by a gradient liquid chromatography-tandem mass spectrometry (LC–MS/MS) method with a run time of 36 s. The method is linear between 0 and 200 HbA1c/mol Hb (IFCC) with a correlation coefficient of 0.999, providing an inter-day reproducibility between 1.3 and 2.3% CV, and comparable with results from analysis of the same samples on the Roche Cobas® c 513 clinical analyzer with a correlation coefficient of 0.998. In two alternative detection workflows that were not characterized in detail, the same digested samples were purified by a magnetic bead-based solid-phase extraction (SPE) method requiring about 10 min and then analyzed using either an isocratic LC–MS/MS method or a flow injection analysis (FIA)-MS/MS method with run times of 12 s and 18 s, respectively. Our work demonstrates the feasibility of LC–MS-based methods for HbA1c determination that minimize the time required for sample preparation and measurement while preserving analytical performance and are thereby more suitable for routine clinical settings compared to traditional methods which require up to 25 h and 23 min, respectively, to prepare and measure samples. Graphical abstract

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Comparability of different methods of glycated hemoglobin measurement for samples of patients with variant and non-variant hemoglobin

Cited by 6 publications

References 13 publications

Analytical performance evaluation of the Mindray enzymatic assay for hemoglobin A1c measurement

Analytical performance evaluation of the Mindray enzymatic assay for hemoglobin A1c measurement

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Early developments toward HbA1c determination in whole blood by high-speed sample preparation and LC–MS/MS analysis

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