Comparable sensitivities for detection of HIV-1 reverse transcriptase (RT) and other polymerases by RT assays requiring no radioisotopic materials

Sano, Kohei; Odawara, Fumitomo; Nakano, Takashi; Morimatsu, Shinichi; Nakamura, Tsumukata; Saitoh, Y.; Jiang, Yan; Misaki, Hideo; Sakai, Yutaka; Nakai, Masuyo

doi:10.1016/0166-0934(95)00028-s

Cited by 12 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…19 To increase the signal detection, therefore allowing the specificity improvement of RT-based assays, product-enhanced RT (PERT) assays have been designed, where neosynthesized cDNAs were further amplified by standard PCR. [20][21][22][23] Newest PERT generations have also been performed in the design of qPCR by the addition of Taqman-based fluorescent oligonucleotide probes. [24][25][26] Whereas the sensitivity of first generation RT assays ranges from 10 4 to 10 6 viral particles, 27 it decreases to 1-10 virions when PERT assays are used.…”

Section: Lentiviral Vector Titration From Rt Activitymentioning

confidence: 99%

Real-time quantitative PCR for the design of lentiviral vector analytical assays

Delenda

Gaillard

2005

Gene Ther

View full text Add to dashboard Cite

From the recent and emerging concerns for approving lentiviral vector-mediated gene transfer in human clinical applications, several analytical methods have been applied in preclinical models to address the lentiviral vector load in batches, cells or tissues. This review points out the oldest generation methods (blots, RT activity, standard PCR) as well as a full description of the newest real-time quantitative PCR (qPCR) applications. Combinations of primer and probe sequences, which have worked in the lentiviral amplification context, have been included in the effort to dress an exhaustive list. Also, great variations have been observed from interlaboratory results, we have tempted to compare between them the different analytical methods that have been used to consider (i) the titration of lentiviral vector batches, (ii) the absence of the susceptible emerging replicative lentiviruses or (iii) the lentiviral vector biodistribution in the organism.

show abstract

Section: Lentiviral Vector Titration From Rt Activitymentioning

confidence: 99%

Real-time quantitative PCR for the design of lentiviral vector analytical assays

Delenda

Gaillard

2005

Gene Ther

View full text Add to dashboard Cite

show abstract

“…An advantage of RT assays is that they detect HIV-1, HIV-2, or SIV. Extremely sensitive, nonradioactive RT ELISA kits can also be purchased from Cavidi Tech Inc. (http://www.cavidi.se; Porstmann et al, 1991;Sano et al, 1995).…”

Section: Critical Parameters and Troubleshootingmentioning

confidence: 99%

Human Immunodeficiency Viruses: Propagation, Quantification, and Storage

2013

View full text Add to dashboard Cite

show abstract

“…An advantage of RT assays is that they detect HIV‐1, HIV‐2, or SIV. Extremely sensitive, nonradioactive RT ELISA kits can also be purchased from Cavidi Tech Inc. (http://www.cavidi.com; Porstmann et al, , Sano et al, ).…”

Section: Commentarymentioning

confidence: 99%