Comparação da reação de imunofluorescência indireta e do teste de aglutinação modificado na detecção de anticorpos anti-Toxoplasma gondii em ratos

Cola, Gustavo Antonio; Garcia, João Luís; Costa, Letícia da; Ruffolo, Bruno Bergamo; Navarro, Italmar Teodorico; Freire, Roberta Lemos

doi:10.5433/1679-0359.2010v31n3p717

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…The same tests when used with other species' samples presented a fair agreement. When comparing the IFA and the MAT using mice serum a Kappa coefficient of 0.55, was found when adopting a cutoff point of 50 IU (Cola et al, 2010); Silva et al (Silva et al, 2002) found Kappa ranging from 0.59 to 0.84 when testing the IFA and the MAT with 16 IU, 64 IU and 256 IU titers in the serum of ewe, goats, cats and dogs. A sensitivity of 85% and specificity of 100% of IFA was reported (Franco et al, 2003) when comparing to MAT results in dog serum of the state of Roraima.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Toxoplasma gondii infections in swine of non-tecnified rearing farms of the northeastern region of the state of São Paulo, Brazil and associated risk factors

Oliveira

Almeida

Sartori

et al. 2019

Parasite Epidemiology and Control

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Toxoplasmosis is a zoonosis present worldwide. Its protozoal aethiological agent, Toxoplasma gondii, has the ability to infect several homeothermic animals and mainly human beings. The consumption of raw or undercooked meat products containing T. gondii cysts, consumption of vegetables without washing and using non-treated water are risk factors associated to the occurrence of human toxoplasmosis. Furthermore, raw or undercooked pork is an important infection source of T. gondii to humans. Due to the importance of toxoplamosis in public health, this study focused on establish the prevalence of the disease in non-technified swine herds in the northeastern region of the State of São Paulo, Brazil, using Modified Agglutination Test (MAT) and the Indirect Immunofluorescence Assay (IFA) and the risk factors for its occurrence. In addition, the agreement among both diagnostic tests was evaluated. A low prevalence of toxoplasmosis was found at animal level (7.02%). The Fisher's exact test detected correlation between positive cases with the presence of food garden in the farm (p = 0.01) and the use of non-treated water to irrigate the food garden (p = 0.005). The agreement among tests was considered moderate (Kappa index = 0.5). The results show that toxoplasmosis is a risk for humans who consume under cooked pork meat in this region and an acceptable agreement between MAT and IFA tests.

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Section: Discussionmentioning

confidence: 99%

Prevalence of Toxoplasma gondii infections in swine of non-tecnified rearing farms of the northeastern region of the state of São Paulo, Brazil and associated risk factors

Oliveira

Almeida

Sartori

et al. 2019

Parasite Epidemiology and Control

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“…Antibodies to T. gondii in small wild rodents from Brazil were reported in Proechimys sp., Rattus rattus, Nectomys rattus, Akodon cursor, and Thrichomys laurentius SIQUEIRA et al, 2013). In urban area R. rattus presented antibodies to T. gondii (FERRARONI & MARZOCHI, 1980;COLA et al, 2010), and viable parasites were isolated by bioassay and detected by PCR in Rattus norvegicus and Mus musculus (MURADIAN et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Toxoplasma gondii antibodies in wild rodents and marsupials from the Atlantic Forest, state of São Paulo, Brazil

Gennari

Ogrzewalska

Soares

et al. 2015

Rev. Bras. Parasitol. Vet.

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Toxoplasma gondii is a protozoan parasite that infects a large spectrum of warm-blooded animals, including humans. Small rodents and marsupials play an important role in the epidemiology of T. gondii because they are sources of infection for domestic and feral cats. Serum samples from 151 rodents and 48 marsupials, captured in the Atlantic Forest, São Paulo State, southeastern Brazil, were analyzed for the presence of T. gondii antibodies. Antibodies detected by the modified agglutination test (MAT ≥ 25) were found in 8.6% (13/151) of the rodents and 10.4% (5/48) of the marsupials, with titers ranging from 25 to 6400 and from 25 to 3200, respectively for the rodents and marsupials. Three of the eight species of rodents (Akodon spp., Oligoryzomys nigripes and Rattus norvegicus), and one from the four marsupial species (Didelphis aurita) presented positive animals. T. gondii was described for the first time in the rodent Oligoryzomys nigripes.Keywords: Atlantic Forest, Brazil, marsupials, rodents, Toxoplasma gondii. ResumoToxoplasma gondii é um protozoário parasita que infecta animais de sangue quente, incluindo seres humanos. Pequenos roedores e marsupiais têm papel importante na epidemiologia do T. gondii, pois são fontes de infecção para os felídeos domésticos e selvagens. Amostras de soro de 151 roedores e 48 marsupiais, capturados na Mata Atlântica, Estado de São Paulo, Sudeste do Brasil, foram analisadas para a pesquisa de anticorpos anti-T. gondii. Os anticorpos foram detectados pelo Teste de Aglutinação Modificada (MAT ≥ 25), com 8,6% (13/151) dos roedores e 10,4% (5/48) dos marsupiais soropositivos, com títulos variando de 25 a 6.400 e de 25 a 3.200, respectivamente, para os roedores e os marsupiais. Três das oito espécies de roedores (Akodon spp., Oligoryzomys nigripes e Rattus norvegicus) e uma das quatro espécies de marsupiais (Didelphis aurita) apresentaram animais positivos. A presença de anticorpos anti-T. gondii foi descrita pela primeira vez no roedor Oligoryzomys nigripes.

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“…Toxoplasma gondii naturally infects several species of marsupials and rodents, however, the frequencies of such infections can vary according to the region and the diagnostic technique used. Studies in Brazil have shown an infection rate ranging from 5.7% to 61.1% in wild rodents and from 4.7% to 63.6% in marsupials (Ferraroni & Marzochi, 1980;Salata et al, 1985;Yai et al, 2003;Cola et al, 2010, Costa et al, 2012Vitaliano et al, 2014;Fournier et al, 2014;Gennari et al, 2015;Abreu et al, 2016;Horta et al 2016). Research on this parasite in Chiroptera is even more scarce, although there are records in São Paulo (Cabral et al, 2013; showing more than 36.6% positives.…”

Section: Introductionmentioning

confidence: 99%

Survey of anti-Toxoplasma gondii antibodies in wild mammals captured from Atlantic Forest fragments in Bahia, northeastern Brazil

Brito

Rocha

Silva

et al. 2020

Rev. Bras. Parasitol. Vet.

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The objective of this study was to investigate the frequency of antibodies to Toxoplasma gondii present in wild mammals that were trap captured in forest fragments in the State of Bahia, northeastern Brazil. A total of 368 individuals (246 rodents, 104 marsupials and 18 bats) were captured using live catch traps. Serum samples were tested using the modified agglutination test, with a cut-off point at 1:25 dilution. The total occurrence of antibodies to T. gondii was 10.6% (39/368), being 16.3% (17/104) in marsupials, 8.5% (21/246) in rodents, and 5.5% (1/18) in bats. Antibody titers varied between 25 and 50 for rodents, between 25 and 400 for marsupials, and were 25 for bats. This is the first report on antibodies to T. gondii in certain rodent species (Thaptomys nigrita, Hylaeamys laticeps, and Cerradomys subflavus), marsupial species (Monodelphis americana, Gracilinanus microtarsus, Gracilinanus agilis and Marmosops incanus), and bats of the genus Rhynchonycteris. The presence of antibodies to T. gondii in wild mammals demonstrates the possibility of these animals as sentinels of toxoplasmosis, especially on regions under high anthropogenic effect.

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Comparação da reação de imunofluorescência indireta e do teste de aglutinação modificado na detecção de anticorpos anti-Toxoplasma gondii em ratos

Cited by 6 publications

References 9 publications

Prevalence of Toxoplasma gondii infections in swine of non-tecnified rearing farms of the northeastern region of the state of São Paulo, Brazil and associated risk factors

Prevalence of Toxoplasma gondii infections in swine of non-tecnified rearing farms of the northeastern region of the state of São Paulo, Brazil and associated risk factors

Toxoplasma gondii antibodies in wild rodents and marsupials from the Atlantic Forest, state of São Paulo, Brazil

Survey of anti-Toxoplasma gondii antibodies in wild mammals captured from Atlantic Forest fragments in Bahia, northeastern Brazil

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