Comparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco

Abstract

Objetivou-se com este estudo comparar genotipicamente 35 isolados de Corynebacterium pseudotuberculosis recuperados de conteúdo de abscessos de caprinos e ovinos com linfadenite caseosa, procedentes de cinco municípios localizados no Sertão de Pernambuco, Brasil. Utilizou-se a técnica de fingerprint RFLP-PCR com as enzimas de restrição Hpy-Ch4 e Msp1 aplicada ao gene rpoB e as enzimas Pst I e Msp I para o gene pld. Não houve diferença nos padrões de fragmentos de bandas entre os isolados, independente da espécie hospedeira ou da área geográfica estudada, definindo-se um padrão genotípico homogêneo de C. pseudotuberculosis responsável por abscessos superficiais na região.

Study of bacteria isolated from the foot pad of Spheniscus magellanicus with and without bumblefoot

“…The same repeats with penicillin, drug with higher percentage of resistance of the tested strains. Corroborating with previous studies with bacteria of the genus Corynebacterium (Abreu et al, 2008), Staphylococcus (Moreira et al, 2010) and E. coli (Baccaro, 2002).…”

“…Although the bacterial resistance configures a problem in population often submitted to antibiotic therapy (Baccaro et al, 2002), this reality has also been related in wild birds (Santos et al, 2010), corroborating this study, in which the strains isolated from Magellanic penguins without previous antibiotics treatment presented varied susceptibility facing the tested antimicrobial. Therefore, using the data obtained in this study, enrofloxacin and cephalosporin may be indicated in empiric bumblefoot treatment, while the use of penicillin and streptomycin is not indicated, since drugs with more than 20% of local resistance are not recommended for empirical treatment (Naber, 2000).…”

“…and Corynebacterium spp. (Baccaro, 2002). But in this study, the resistance rates against the three genus demonstrated that the antimicrobial should not be indicated in the treatment of bumblefoot involving these agents.…”

“…However, in the present study, the E. coli strains susceptibility rate were null, as already demonstrated by Lara (2008). Baccaro et al (2002) attributes this resistance to the indiscriminate use of antibiotics in veterinary medicine. However, there are studies that show susceptibility rates of 60% to 80% (Vieira et al, 2007;Moreira, 2010) Enrofloxacin was the second antibiotic with the best in vitro response, with a higher E. coli susceptibility rate than expected, according to the literature (Baccaro, 2002), and lower Staphylococcus spp.…”

Citologia aspirativa no diagnóstico da linfadenite em ovinos

“…A linfadenite caseosa ϐigura dentre as afecções mais frequentes na criação de pequenos ruminantes no Brasil (Dorella et al 2006, Abreu et al 2008, Carvalho et al 2008. A doença se caracteriza pela formação de abscessos em linfonodos, causada predominantemente por Corynebacterium pseudotuberculosis (Dorella et al 2006).…”