Comparation of Physiology, Diameter of Ovary and Follicle at PO Cows Treated Different Method of Estrus Synchronization

Abstract

One of the biotechnology reproductive used is estrus synchronization. This study determined the body’s physiological, estradiol level, and ovarian and follicle diameter in Peranakan Ongole (PO) cow estrus synchronization using modified progesterone-PGF2α and double injection of PGF2α. This study used 10 PO cows which were divided into two groups. Group 1, cows were given a modified intravaginal sponge containing 250 mg progesterone/sponge for 6 days, followed by intramuscularly injection of PGF2ɑ 25 mg/cow on day sixth. Appearance of estrus was observed on day 7th -9th. Group 2, cows were given a double injection 25 mg/cow of PGF2ɑ intramuscularly at 14-day intervals, then estrus was observed on day 15th -17th. The vaginal temperature was checked using a thermometer, the respiratory frequency was checked by inspection of thorax movements, and the pulse rate was checked by palpation of the coccygeal artery. Ovarian and Graafian follicle diameters were examined using ultrasonography. The data were analyzed using either T-Test or descriptive test. The results showed that in cows that were synchronized to estrus using combination of modified progesterone-PGF2ɑ and double injection of PGF2ɑ, the vaginal temperature, pulse rate, and respiration frequency were 36.9±0.98 and 37.34±0.83, 57.60±12.52 and 74.80±26.59, 30.40±10.43 and 20.00±2.83 respectively. There was no significant difference in ovarian and follicle diameter (P>0.05). The conclusion was the results of body physiology were in normal conditions but still below the average of cows undergoing estrus, and there were no significant differences in ovarian and Graafian follicle diameters.