Comparation of the effects of different 5′-untranslated regions (UTRs) on gene expression in HEK293 cells

Zhang, Xuemei; Zha, Guang-Ming; Wang, Jiang; Wang, Xinjian; Song, Shuang; Shu, Jing-Chao; Chu, Bei-Bei; Yang, Gangyi

doi:10.1007/s10529-016-2199-8

Cited by 5 publications

(6 citation statements)

References 17 publications

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“…Both rs2803495 and rs2803496 variants are in the 5′-untranslated region (UTR) of TREML4 . The 5′-UTR contains regulatory regions, such as selenocysteine insertion sequence elements, AU-rich elements, riboswitches, and microRNA-binding sites that affect translational efficiency, subcellular localization, and mRNA stability 22 . Therefore, the 5′-UTR plays an important role in controlling gene expression 23 , and polymorphisms in this region may influence mRNA stability and, consequently, gene expression levels.…”

Section: Discussionmentioning

confidence: 99%

TREML4 mRNA Expression and Polymorphisms in Blood Leukocytes are Associated with Atherosclerotic Lesion Extension in Coronary Artery Disease

Duarte

Miranda

Cruz

et al. 2019

Sci Rep

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Members of the triggering receptor expressed on myeloid cells (TREM) family are associated with atherosclerosis risk and progression. TREML4 is upregulated in the early phase of acute coronary syndrome. We investigated the relationship between the mRNA expression of 13 genes in blood leukocytes, TREML4 polymorphisms, and coronary artery lesion extension (Friesinger index) in patients with coronary artery disease (CAD) (n = 137). TREML4 rs2803495 (A > G) and rs2803496 (T > C) variants and leukocyte mRNA expression were analysed by qRT-PCR. TREML4 expression was higher in patients with major coronary artery lesions than in subjects without or with low and intermediate lesions ( p < 0.05). However, TREML4 polymorphisms were not associated with coronary lesion extent. Presence of the rs2803495 G allele was not associated with increased TREML4 mRNA expression. Patients carrying the rs2803496 C allele (TC/CC genotypes) were more likely to express TREML4 mRNA than non-C allele carriers (allele C: OR 7.3, and 95% CI 1.9–27.5, p = 0.03). In conclusion, increased TREML4 mRNA expression in blood leukocytes is influenced by gene polymorphisms and is associated with more severe coronary artery lesions, suggesting its potential as a biomarker of the extent of coronary lesions in patients with CAD.

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Section: Discussionmentioning

confidence: 99%

TREML4 mRNA Expression and Polymorphisms in Blood Leukocytes are Associated with Atherosclerotic Lesion Extension in Coronary Artery Disease

Duarte

Miranda

Cruz

et al. 2019

Sci Rep

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“…4C, and 4D). Interestingly, hybrid intron and EF-1α intron A, which were originally reported to have the function of upregulating gene expression (Melcher, Grosch et al 2002, Zhang, Zha et al 2016), instead of working as expected, showed signi cant inhibition of eGFP gene expression in L929 and MARC145 cells after combination with the CMV enhancer and EF-1α core promoter.…”

Section: Resultsmentioning

confidence: 73%

“…In addition, introns can also improve the translation e ciency of mRNA (Shaul 2017). It is commonly believed that UTR is primarily responsible for regulating the expression levels of post-transcriptional genes by altering the stability of mRNA, translation e ciency and subcellular localization, and it has been found that the 5 '-UTR of mRNA affects the expression of prokaryotes and eukaryotes at the translational level mainly by forming secondary structures (Crook, Freeman et al 2011, Zhang, Zha et al 2016. For the 3'UTR and terminator, studies of prokaryotic expression systems have found that both terminator and engineered 3'UTR elements can drastically alter the level of heterologous expression (Cambray, Guimaraes et al 2013).…”

Section: Optimization Of Expression Vectors Is the Main Means To Impr...mentioning

confidence: 99%

Comparison of the effects of different combinations of expression regulatory elements on gene expression in mammalian cells

Zhou

Zhang

et al. 2022

Preprint

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Background At present, the function of numerous individual regulatory elements has been characterized extensively, but the combined effect of various cis-regulatory elements on gene expression in mammalian cells has been less discussed. Results In the present study, we examined the function of the combined effect of various cis-regulatory elements on gene expression in mammalian cells. To detect the function of different combinations of cis-acting elements on gene expression, we compared the effects of four promoters (PGK, Polr2a and EF-1α core promoter), two enhancers (CMV and SV40 enhancer), two introns (EF-1α intron A and hybrid intron), two terminators (CYC1 and TEF terminator) and their different combinations on gene expression. We constructed expression vectors with different combinations of these regulatory elements and detect the expression of eGFP by fluorescence intensity contrast and western blotting. Here we report that different combinations of cis-acting elements have a significant effect on protein expression. The vector with CMV enhancer, EF-1α core promoter and TEF terminator can express about three times higher eGFP than the unmodified vector in different animal cells. Conclusions We demonstrate that a direct combination of multiple regulatory elements capable of regulating gene expression did not exhibit synergistic effects. Our results show that regulation of protein expression can be achieved by optimizing the combination of cis-acting elements, and these findings can be used in biological applications that require regulation of gene expression.

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“…The two significant SNPs divided the 77 lines into two major haplotypes (Hap1: AA; Hap2: CC), a t -test showed that the BSH-LTC (1.178) of Hap1 was significantly (p< 0.05) lower than that (1.417) of Hap 2 ( Figure 3B ). In contrast to synonymous variants and intron_variants, those in the 5’ UTR tend to cause changes in gene transcriptional levels and thus lead to phenotypic variations ( Bashirullah et al., 2001 ; Zhang et al., 2016b ). We then detected the expression of ZmbZIP107 in the two contrasting haplotypes under Pb treatment by using RT-qPCR.…”

Section: Resultsmentioning

confidence: 99%

Association mapping uncovers maize ZmbZIP107 regulating root system architecture and lead absorption under lead stress

Hou

Zhang

et al. 2022

Front. Plant Sci.

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Lead (Pb) is a highly toxic contaminant to living organisms and the environment. Excessive Pb in soils affects crop yield and quality, thus threatening human health via the food chain. Herein, we investigated Pb tolerance among a maize association panel using root bushiness (BSH) under Pb treatment as an indicator. Through a genome-wide association study of relative BSH, we identified four single nucleotide polymorphisms (SNPs) and 30 candidate genes associated with Pb tolerance in maize seedlings. Transcriptome analysis showed that four of the 30 genes were differentially responsive to Pb treatment between two maize lines with contrasting Pb tolerance. Among these, the ZmbZIP107 transcription factor was confirmed as the key gene controlling maize tolerance to Pb by using gene-based association studies. Two 5’ UTR_variants in ZmbZIP107 affected its expression level and Pb tolerance among different maize lines. ZmbZIP107 protein was specifically targeted to the nucleus and ZmbZIP107 mRNA showed the highest expression in maize seedling roots among different tissues. Heterologous expression of ZmbZIP107 enhanced rice tolerance to Pb stress and decreased Pb absorption in the roots. Our study provided the basis for revelation of the molecular mechanism underlying Pb tolerance and contributed to cultivation of Pb-tolerant varieties in maize.

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Comparation of the effects of different 5′-untranslated regions (UTRs) on gene expression in HEK293 cells

Abstract: Different UTRs can significantly affect protein expression. Additionally, the findings also will be useful in biological applications that require tuning of gene expression and system optimization.

Cited by 5 publications

References 17 publications

TREML4 mRNA Expression and Polymorphisms in Blood Leukocytes are Associated with Atherosclerotic Lesion Extension in Coronary Artery Disease

TREML4 mRNA Expression and Polymorphisms in Blood Leukocytes are Associated with Atherosclerotic Lesion Extension in Coronary Artery Disease

Comparison of the effects of different combinations of expression regulatory elements on gene expression in mammalian cells

Association mapping uncovers maize ZmbZIP107 regulating root system architecture and lead absorption under lead stress

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