Comparative accounts of probiotic characteristics of Bacillus spp. isolated from food wastes

Patel, Anil Kumar; Ahire, Jayesh J.; Pawar, Sharad; Chaudhari, Bhushan L.; Chincholkar, S. B.

doi:10.1016/j.foodres.2009.01.013

Cited by 96 publications

(71 citation statements)

References 33 publications

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“…Antibiotic susceptibility is considered as one of the most important characteristics for probiotic bacteria, since the resistance genes could be transferred to pathogenic bacteria in the intestinal tract and thus contribute to the creation of multidrugresistant pathogens (Patel et al 2009;Schwarz et al 2001;Hong et al 2005;Barbosa et al 2005). This transfer mostly occurs by plasmid-borne resistance genes (Schwarz et al 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, probiotic strains displaying stronger adhesion to the GIT are considered to better modify the normal intestinal microbiota, prevent the growth of pathogenic bacteria by competitive exclusion, and induce immune system compared to the non-adherent ones (Saarela et al 2000;Patel et al 2009). The HT-29 cell line is used as a model for the testing of adhesion of probiotic bacteria, as these cells differentiate into enterocytes (Saarela et al 2000;Patel et al 2009). Thus, we used the HT-29 colon carcinoma cell line for the assessment of cell adhesion by the prospective probiotic strains.…”

Section: Discussionmentioning

confidence: 99%

“…Resistance to simulated gastric fluids Vegetative cells of the isolates were tested for survival capacity in simulated gastric fluids as described by Barbosa et al (2005), Hong et al (2008), and Patel et al (2009) with minor modifications. Briefly, bacteria grown overnight were inoculated in LB broth (pH=2.0, adjusted with 37 % HCl) and incubated at 37°C and 125 rpm for 30 min.…”

Section: Spore Formationmentioning

confidence: 99%

“…Samples were then serially diluted, spread on LB agar plates and incubated at 37°C for 24 h. Colonies were counted and compared with those of control cultures grown in unmodified LB broth. Spore tolerance to simulated gastric fluids was also tested as described by Barbosa et al (2005), Duc et al (2004), Hong et al (2008), andPatel et al (2009). Approximately 10 8 -10 9 purified spores per ml were resuspended in 0.85 % NaCl solution (pH=2, adjusted with 37 % HCl), incubated and spread on LB agar plates as described for vegetative cells.…”

Section: Spore Formationmentioning

confidence: 99%

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Screening and selection of novel animal probiotics isolated from bovine chyme

et al. 2012

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Probiotics, gut-colonizing microorganisms capable of conferring a number of health benefits to their hosts, are highly desirable as animal feed supplements. Members of the Gram-positive genus Bacillus are often utilized as probiotics, since endospores formed by those bacteria render them highly resistant to environmental extremes and therefore capable of surviving gastrointestinal tract conditions. In this study, 84 distinct bacterial colonies were obtained from bovine chyme and 29 isolates were determined as Bacillus species. These isolates were principally screened for their antimicrobial activity against a group of two Gram-positive and four Gram-negative bacteria, including known human and animal pathogens such as Salmonella enterica, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Seven strains displaying strong antimicrobial activity against the test cohort were further evaluated for other properties desirable from animal probiotics, including high spore-forming capacity and adhesiveness, resistance to pH extremes and ability to form biofilms. The isolates were found to resist simulated gastrointestinal conditions and most of the antibiotics tested. In addition, plasmid presence was checked and cytotoxicity tests were performed to evaluate the potential risks of antibiotic resistance transfer and unintended pathogenic effects on host, respectively. We propose that the bacterial isolates are suitable for use as animal probiotics.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Spore Formationmentioning

confidence: 99%

Section: Spore Formationmentioning

confidence: 99%

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Screening and selection of novel animal probiotics isolated from bovine chyme

et al. 2012

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“…Based on the in vitro safety evaluation, CM40 appears to be safer than other isolates. However, other evidence has shown that non-pathogenic Bacillus strains Natto and HU36, that display toxicity to mammalian cells, and Bacillus strains DET6 and DET9, which cause complete lysis of red blood cells, do not show any negative side effects in the case of animals (Hong et al, 2008;Patel et al, 2009). …”

Section: Safety Evaluation Of Bacillus Isolatesmentioning

confidence: 99%

<i>In vitro</i> evaluation of candidate <i>Bacillus</i> spp. for animal feed

Mingmongkolchai

Panbangred

2017

J. Gen. Appl. Microbiol.

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The aim of this study was to select aerobic sporeformers for animal feed based on their in vitro probiotic potential, including their enzyme-producing ability and safety assessment. Seven isolates out of 187 spore-forming bacteria were selected for their ability to produce cellulase (89.21-1668.32 U/ ml), xylanase (1399.68-4351.10 U/ml), and phytase (2.72-28.70 U/ml). Among seven isolates, five had activities towards a broad range of p-nitrophenyl esters with acyl chain lengths from C2 to C12. The probiotic properties of all selected isolates varied with respect to their acid and bile salt tolerance under simulated gastrointestinal tract (GIT) conditions, and their adherence ability to human intestinal cell lines (Caco-2 and HT-29). The safety assessment revealed that the isolate CM40 was not cytotoxic to Caco-2 and HT-29, did not exhibit hemolytic activity, carried no enterotoxin or emetic toxin genes, and was susceptible to ten antibiotics, including six key antibiotics (chloramphenicol, erythromycin, gentamicin, tetracycline, streptomycin, and kanamycin) as recommended by the European Food Safety Authority (EFSA). Co-incubation of isolate CM40 with enteric bacteria (Salmonella Typhi, Salmonella Enteritidis 1781, and Escherichia coli) demonstrated that CM40 significantly decreased the number of pathogens (about 30-48%) adhering to Caco-2 and HT-29 (P < 0.05). Analysis of gene encoding 16S rRNA, gyrase A (gyrA) and the cheA histidine kinase revealed that CM40 belongs to Bacillus subtilis. On the basis of probiotic properties and basic safety aspects, the B. subtilis strain CM40 was found to possess desirable in vitro probiotic properties, and may be a potential candidate for supplementation of animal feed.

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